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Genetic analysis and identification of QTLs for resistance to cucumber mosaic virus in chili pepper (Capsicum annuum L.)

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Abstract

A pepper (Capsicum annuum) inbred line ‘BJ0747-1-3-1-1’ was found to exhibit resistance to a cucumber mosaic virus (CMV) local isolate (CMV-HB). In order to exploit the genetic mechanism of CMV resistance in this pepper line, inheritance and genetic linkages of CMV resistance were studied. The CMV-resistant ‘BJ0747-1-3-1-1’ (P1) was crossed to the susceptible line ‘XJ0630-2-1-2-1-1’ (P2), and the F2 and back-cross populations (B1 and B2) were generated for segregation analysis following a mixed inheritance model. Analysis of the segregation data revealed that CMV resistance in ‘BJ0747-1-3-1-1’ is controlled by two partially additive-dominant major genes and additive-dominant polygenes. Analysis of the results from two growth seasons also identified two stable and major QTLs for CMV resistance that collectively explained 55 % of the trait variation. One of the two major QTLs was found on linkage group 8 (LG8) between markers TS52 and HpmsE1-43, and accounted for 37.7–43.5 % of the variation in the two inoculation experiments. The other QTL on LG4 between markers UBC843 and S74 was found to be responsible for 10.7–11.2 % of the trait variation. QTLs with minor effects on CMV resistance were also identified. This work provides an example for genetic analysis and QTL mapping of other disease-resistance genes in pepper and the findings should be useful for molecular marker-assisted breeding programs that aim at developing disease-resistant cultivars in peppers.

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Acknowledgments

We thank Dr Zonglie Hong from University of Idaho for critical reading of the manuscript. This work was supported by a grant from the KIC Hubei (No. 2009BFA005), ISTC MOST (No. 2011DFB31620) and SFYS HAAS (No. 2012NKYJJ04).

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Minghua Yao & Zhibiao Ye

  2. Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan, 430064, People’s Republic of China

    Minghua Yao, Ning Li & Fei Wang

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  1. Minghua Yao
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  2. Ning Li
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  3. Fei Wang
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  4. Zhibiao Ye
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Correspondence to Zhibiao Ye.

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Yao, M., Li, N., Wang, F. et al. Genetic analysis and identification of QTLs for resistance to cucumber mosaic virus in chili pepper (Capsicum annuum L.). Euphytica 193, 135–145 (2013). https://doi.org/10.1007/s10681-013-0953-8

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