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Seed transmission of Tomato yellow leaf curl virus in sweet pepper (Capsicum annuum)

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Abstract

Sweet pepper (Capsicum annuum) is a popular crop worldwide and an asymptomatic host of the begomovirus (Geminiviridae) Tomato yellow leaf curl virus (TYLCV). A previous study showed that TYLCV could be transmitted by the seeds of tomato plants, but this phenomenon has not been confirmed in other plants. In 2015, four different cultivars of sweet pepper (‘Super Yellow,’ ‘Super Red,’ ‘Sunnyez’ and ‘Cupra’) known to be susceptible to TYLCV were agro-inoculated with a TYLCV infectious clone. Three months after inoculation, the leaves of the ‘Super Yellow’ cultivar showed 80% (8/10) susceptibility and the other three sweet pepper cultivars showed 30 to 50% susceptibilities. All of the ‘Super Yellow’ seed bunches (five seeds per bunch) from plants whose leaves were confirmed to be TYLCV-infected were also TYLCV-infected (8/8). The seeds of other cultivars showed 20 to 40% susceptibilities. Virus transmission rates were also verified with 10 bunches of seedlings for each cultivar (five seedlings per pool). Eight bunches of ‘Super Yellow’ seedlings (8/10) were confirmed to be TYLCV-infected and one to three bunches of each of the other cultivar seedlings were also infected. Viral replication in TYLCV-infected seeds and seedlings was confirmed via strand-specific amplification using virion-sense- and complementary-sense-specific primer sets. This is the first report of TYLCV seed transmission in sweet pepper plants and among non-tomato plants. Because sweet pepper is an asymptomatic host of TYLCV, seeds infected with TYLCV could act as a silent invader of tomatoes and other crops.

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Acknowledgements

This research was supported by a grant (311058-05-5-HD140) from the Agricultural Biotechnology Development Program, Ministry of Agriculture, Food and Rural Affairs of the Republic of Korea and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2016R1C1B2014391).

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Author notes

  1. Eui-Joon Kil and Jungho Park contributed equally to this work.

Authors and Affiliations

  1. Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, 16419, South Korea

    Eui-Joon Kil, Jungho Park, Eun-Young Choi, Hee-Seong Byun & Sukchan Lee

  2. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, South Korea

    Kyeong-Yeoll Lee

  3. Research and Development Bureau, Gyeongsangnam-do Agricultural Research & Extension Services, Jinju, 52733, South Korea

    Chul Geon An

  4. Gyeongsangbuk-do Agricultural Research & Extension Services, Institute for Bioresources Research, Andong, 36614, South Korea

    Joong-Hwan Lee

  5. Crop Protection Division, Rural Development Administration, National Institute of Agricultural Sciences, Wanju, 55365, South Korea

    Gwan-Seok Lee & Hong-Soo Choi

  6. Highland Agriculture Research Institute, Rural Development Administration, National Institute of Crop Science, Pyeongchang, 25342, South Korea

    Chang-Seok Kim

  7. Future High-Technology Agriculture Research Division, Research and Development Bureau, Chungcheongnam-do Agricultural Research & Extension Services, 167 Chusa-ro, Jonggyeong-ri, Yesan, 32418, South Korea

    Ji-Kwang Kim

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  1. Eui-Joon Kil
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  2. Jungho Park
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  12. Sukchan Lee
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Corresponding authors

Correspondence to Ji-Kwang Kim or Sukchan Lee.

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This research was supported by a grant (311058-05-5-HD140) from the Agricultural Biotechnology Development Program, Ministry of Agriculture, Food and Rural Affairs of the Republic of Korea and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2016R1C1B2014391).

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Kil, EJ., Park, J., Choi, EY. et al. Seed transmission of Tomato yellow leaf curl virus in sweet pepper (Capsicum annuum). Eur J Plant Pathol 150, 759–764 (2018). https://doi.org/10.1007/s10658-017-1304-8

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  • DOI: https://doi.org/10.1007/s10658-017-1304-8

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