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Antibacterial activity of petroleum ether fraction from Laminaria japonica extracts against Clavibacter michiganensis subsp. sepedonicus

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Abstract

We prepared an ethanol extract from Laminaria japonica, and obtained five fractions from the extract using various solvents. We investigated the antibacterial activities of the fractions against Clavibacter michiganensis subsp. sepedonicus (Spieckermann & Kotthoff) Davis et al., the causal agent of bacterial ring rot of potato (BRR). Among all the fractions, the petroleum ether fraction showed the strongest (P < 0.05) antibacterial activity against C. michiganensis subsp. sepedonicus. We analyzed the petroleum ether fraction to detect the components with antibacterial activity, and identified 24 compounds. The main constituents of the petroleum ether extract were esters (35.28 %) and acids (44.84 %). Cell membrane permeability assays, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses revealed that the petroleum ether fraction caused drastic ultrastructural changes in the cells of the pathogen. The petroleum ether fraction caused envelope disruption, cell deformation, the separation of the cell wall from the cell membrane, and the formation of vacuoles. These results clearly indicate that the petroleum ether fraction obtained from L. japonica shows strong antibacterial activity against the causal agent of BRR.

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  1. School of Life Science, Shanxi University, Taiyuan, 030006, China

    Jin Cai, Jia Feng, Feipeng Wang, Qiufeng Xu & Shulian Xie

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  1. Jin Cai
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Cai, J., Feng, J., Wang, F. et al. Antibacterial activity of petroleum ether fraction from Laminaria japonica extracts against Clavibacter michiganensis subsp. sepedonicus . Eur J Plant Pathol 140, 291–300 (2014). https://doi.org/10.1007/s10658-014-0462-1

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