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Acute toxicity assessment of N,N-diethyl-m-toluamide (DEET) on the oxygen flux of the dinoflagellate Gymnodinium instriatum

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Abstract

Despite the ubiquitous occurrence of N,N-diethyl-m-toluamide (DEET) in aquatic systems, assessments evaluating the toxicity of DEET on phytoplankton species are summed to a single study on a unicellular green alga. In particular, the toxicological effects of DEET in dinoflagellates are unknown. In this study, we employed the mixotrophic dinoflagellate Gymnodinium instriatum as a study system to evaluate acute effects of DEET on the oxygen flux of laboratory cultures. This study reports an inhibitory reaction model of DEET described by the equation y = 4.99x0.54, where y represents the percent inhibition of oxygen flux and x represents DEET concentration in mg L−1 (r2 = 0.98). Based on this model, the effective concentration of DEET needed to reduce O2 flux by 50 % (EC50) for this species was found to be at 72.9 mg L−1. The reported EC50 is more than five times lower than the EC50 reported previously for the unicellular green algae Chlorella protothecoides. This study raises the question of the potential toxicological effects of DEET in dinoflagellates, in particular those populations inhabiting systems characterized by low water circulation such as enclosed bays and lagoons.

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References

  • Abou-Donia MB (1996) Neurotoxicity resulting from coexposure to pyridostigmine bromide, DEET, and permethrin: implications of Gulf War chemical exposures. J Toxicol Environ Health Part A 48:35–56

    Article  CAS  Google Scholar 

  • Adams Iii W, Demmig-Adams B, Rosenstiel T, Brightwell A, Ebbert V (2002) Photosynthesis and photoprotection in overwintering plants. Plant Biol 4:545–557

    Article  Google Scholar 

  • Andersen RA (2005) Algal culturing techniques. Academic Press, San Diego

    Google Scholar 

  • Bachman S, Muller-Parker G (2007) Viable algae released by the seastar Dermasterias imbricata feeding on the symbiotic sea anemone Anthopleura elegantissima. Mar Biol 150:369–375

    Article  Google Scholar 

  • Barnes KK, Christenson SC, Kolpin DW, Focazio MJ, Furlong ET, Zaugg SD, Meyer MT, Barber LB (2004) Pharmaceuticals and other organic waste water contaminants within a leachate plume downgradient of a municipal landfill. Groundw Monit Remediat 24:119–126

    Article  CAS  Google Scholar 

  • Brading P, Warner ME, Davey P, Smith DJ, Achterberg EP, Suggett DJ (2011) Differential effects of ocean acidification on growth and photosynthesis among phylotypes of Symbiodinium (Dinophyceae). Limnol Oceanogr 56:927–938

    Article  CAS  Google Scholar 

  • Brooke LT, Call DJ, Geiger DL, Northcott CE (1984) Acute toxicities of organic chemicals to fathead minnows [Pimephales promelas]. University of Wisconsin-Superior

  • Cohu CM, Muller O, Stewart JJ, Demmig-Adams B, Adams WW (2013) Association between minor loading vein architecture and light- and CO(2)-saturated rates of photosynthetic oxygen evolution among Arabidopsis thaliana ecotypes from different latitudes. Front Plant Sci 4:264

    Google Scholar 

  • Corbel V, Stankiewicz M, Pennetier C, Fournier D, Stojan J, Girard E, Dimitrov M, Molgó J, Hougard J-M, Lapied B (2009) Evidence for inhibition of cholinesterases in insect and mammalian nervous systems by the insect repellent deet. BMC Biol 7:47

    Article  Google Scholar 

  • Cordy GE, Duran NL, Bouwer H, Rice RC, Furlong ET, Zaugg SD, Meyer MT, Barber LB, Kolpin DW (2004) Do pharmaceuticals, pathogens, and other organic waste water compounds persist when waste water is used for recharge? Groundw Monit Remediat 24:58–69

    Article  CAS  Google Scholar 

  • Costanzo S, Watkinson A, Murby E, Kolpin D, Sandstrom M (2007) Is there a risk associated with the insect repellent DEET (N,N-diethyl-m-toluamide) commonly found in aquatic environments? Sci Total Environ 384:214–220

    Article  CAS  Google Scholar 

  • Davis EE, Sokolove PG (1976) Lactic acid-sensitive receptors on the antennae of the mosquito, Aedes aegypti. J Comp Physiol A 105:43–54

    Article  CAS  Google Scholar 

  • DeLorenzo ME, Scott GI, Ross PE (2001) Toxicity of pesticides to aquatic microorganisms: a review. Environ Toxicol Chem 20:84–98

    Article  CAS  Google Scholar 

  • Dickens JC, Bohbot JD (2013) Mini review: mode of action of mosquito repellents. Pestic Biochem Physiol 106:149–155

    Article  CAS  Google Scholar 

  • Doherty JD (1979) Insecticides affecting ion transport. Pharmacol Ther 7:123–151

    Article  CAS  Google Scholar 

  • Glassmeyer ST, Furlong ET, Kolpin DW, Cahill JD, Zaugg SD, Werner SL, Meyer MT, Kryak DD (2005) Transport of chemical and microbial compounds from known wastewater discharges: potential for use as indicators of human fecal contamination. Environ Sci Technol 39:5157–5169

    Article  CAS  Google Scholar 

  • Guillard RRL, Ryther JH (1962) Studies of marine planktonic diatoms. I. Cyclotella nana and Detonula confervacea. Can J Microbiol 8:10

    Article  Google Scholar 

  • Hoefnagel MH, Atkin OK, Wiskich JT (1998) Interdependence between chloroplasts and mitochondria in the light and the dark. Bioch Biophys Acta 1366:235–255

    CAS  Google Scholar 

  • Hollister TA, Walsh GE (1973) Differential responses of marine phytoplankton to herbicides: oxygen evolution. Bull Environ Contam Toxicol 9:291–295

    Article  CAS  Google Scholar 

  • Hollister TA, Walsh GE, Forester J (1975) Mirex and marine unicellular algae: accumulation, population growth and oxygen evolution. Bull Environ Contam Toxicol 14:753–759

    Article  CAS  Google Scholar 

  • Hunt D, Treacy M (1998) Pyrrole insecticides: a new class of agriculturally important insecticides functioning as uncouplers of oxidative phosphorylation, insecticides with novel modes of action. Springer, Berlin, pp 138–151

    Google Scholar 

  • Ilivicky J, Casida JE (1969) Uncoupling action of 2,4-dinitrophenols, 2-trifluoromethylbenzimidazoles and certain other pesticide chemicals upon mitochondria from different sources and its relation to toxicity. Biochem Pharmacol 18:1389–1401

    Article  CAS  Google Scholar 

  • Kolpin DW, Furlong ET, Meyer MT, Thurman EM, Zaugg SD, Barber LB, Buxton HT (2002) Pharmaceuticals, hormones, and other organic wastewater contaminants in US streams, 1999–2000: a national reconnaissance. Environ Sci Technol 36:1202–1211

    Article  CAS  Google Scholar 

  • Koren G, Matsui D, Bailey B (2003) DEET-based insect repellents: safety implications for children and pregnant and lactating women. Can Med Assoc J 169:209–212

    Google Scholar 

  • Kupfer D, Fotus J, Philpot R (1975) Effects of pesticides and related compounds on steroid metabolism and function. Crit Rev Toxicol 4:83–124

    CAS  Google Scholar 

  • Mayer FL, Ellersieck MR (1986) Manual of acute toxicity: interpretation and data base for 410 chemicals and 66 species of freshwater animals. US Department of the Interior, Fish and Wildlife Service, Washington, DC

    Google Scholar 

  • McGready R, Hamilton KA, Simpson JA, Cho T, Luxemburger C, Edwards R, Looareesuwan S, White NJ, Nosten F, Lindsay SW (2001) Safety of the insect repellent N,N-diethyl-M-toluamide (DEET) in pregnancy. Am J Trop Med Hyg 65:285–289

    CAS  Google Scholar 

  • Michael A, Grant G (1974) Toxicity of the repellent DEET (N,N-diethyl-metatoluamide) to Gambusia affinis (Baird and Girard). Mosq News 34(1):32–34

    CAS  Google Scholar 

  • Nicholls DG, Ferguson SJ (2002) Bioenergetics. Academic Press, London

    Google Scholar 

  • Pärnik T, Keerberg O (1995) Decarboxylation of primary and end products of photosynthesis at different oxygen concentrations. J Exp Bot 46:1439–1477

    Article  Google Scholar 

  • Relyea RA (2009) A cocktail of contaminants: how mixtures of pesticides at low concentrations affect aquatic communities. Oecologia 159:363–376

    Article  Google Scholar 

  • Sastre MP, Sánchez E, Flores M, Astacio S, Rodríguez J, Santiago M, Olivieri K, Francis V, Núñez J (2013) Population fluctuations of Pyrodinium bahamense and Ceratium furca (Dinophyceae) in Laguna Grande, Puerto Rico, and environmental variables associated during a three-year period. Rev Biol Trop 61:1799–1813

    Google Scholar 

  • Sax, N.I., Lewis Sr, R., 1989. Dangerous Properties of Industrial Materials, in: Reinhold, V.N. (Ed.), Melbourne

  • Seo J, Lee Y-G, Kim S-D, Cha C-J, Ahn J-H, Hur H-G (2005) Biodegradation of the insecticide N,N-diethyl-m-toluamide by fungi: identification and toxicity of metabolites. Arch Environ Contam Toxicol 48:323–328

    Article  CAS  Google Scholar 

  • Simon JY (2014) The toxicology and biochemistry of insecticides. CRC Press, Boca Raton

    Google Scholar 

  • Soler-Figueroa BM, Otero E (2014) The influence of rain regimes and nutrient loading on the abundance of two dinoflagellate species in a tropical bioluminescent bay, Bahía Fosforescente, La Parguera, Puerto Rico. Estuar Coasts 38:84–92

    Article  Google Scholar 

  • Stackelberg PE, Furlong ET, Meyer MT, Zaugg SD, Henderson AK, Reissman DB (2004) Persistence of pharmaceutical compounds and other organic wastewater contaminants in a conventional drinking-water-treatment plant. Sci Total Environ 329:99–113

    Article  CAS  Google Scholar 

  • Tisch M, Schmezer P, Faulde M, Groh A, Maier H (2002) Genotoxicity studies on permethrin, DEET and diazinon in primary human nasal mucosal cells. Eur Arch Otorhinolaryngol 259:150–153

    Article  Google Scholar 

  • Trebst A (1980) Inhibitors in electron flow: tools for the functional and structural localization of carriers and energy conservation sites. Methods Enzymol 69:675–715

    Article  CAS  Google Scholar 

  • USEPA (1998) Reregistration Eligibility Decision (RED) for DEET, Washington, DC

  • USEPA (2000). Pesticide ecotoxicity database (formerly: environmental effects database (EEDB)). In: Office of Pesticide Programs, E.F.a.E.D. (ed), Washington, DC

  • Walker D (1985) Measurement of oxygen and chlorophyll fluorescence. In: Coombs JH, Hall DO, Long SP, Scurlock JMO (ed), Techniques in bioproductivity and photosynthesis: Pergamon International Library of Science, Technology, Engineering and Social Studies, 2nd edn. Pergamon Press, New York, p 95

  • Walker D, Walker R (1987) The use of the oxygen electrode and fluorescence probes in simple measurements of photosynthesis. Packard Publishing, Chichester

    Google Scholar 

  • Walsh GE (1972) Effects of herbicides on photosynthesis and growth of marine unicellular algae. Hyacinth Control J 10:45–48

    Google Scholar 

  • Walsh G (1978) Toxic effects of pollutants on Plankton. Principles of ecotoxicology. Wiley, New York, pp 257–274

    Google Scholar 

  • Walsh GE (1988) Principles of toxicity testing with marine unicellular algae. Environ Toxicol Chem 7:979–987

    Article  CAS  Google Scholar 

  • Wille T, Thiermann H, Worek F (2011) In vitro kinetic interactions of DEET, pyridostigmine and organophosphorus pesticides with human cholinesterases. Chem Biol Interact 190:79–83

    Article  CAS  Google Scholar 

  • Yamano T, Morita S (1995) Effects of pesticides on isolated rat hepatocytes, mitochondria, and microsomes II. Arch Environ Contam Toxicol 28:1–7

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors will like to extent our gratitude to Dr. Joseph J. Torres, who kindly provided both high-resolution respirometry systems used in this study. This research was funded by the National Science Foundation (Grant Number OCE-0727883), awarded to Dr. Joseph J. Torres.

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Authors and Affiliations

  1. Center for Environmental Studies, Virginia Commonwealth University, 1000 W Cary St., Richmond, VA, 23284, USA

    Eloy Martinez

  2. Universidad de Puerto Rico en Humacao, Carretera 908 Km. 1.2, Humacao, PR, 00792, USA

    Sylvia M. Vélez & Miguel P. Sastre

  3. College of Marine Science, University of South Florida, 140 7th Ave S, Saint Petersburg, FL, 33701, USA

    Marietta Mayo

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  1. Eloy Martinez
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Correspondence to Eloy Martinez.

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Martinez, E., Vélez, S.M., Mayo, M. et al. Acute toxicity assessment of N,N-diethyl-m-toluamide (DEET) on the oxygen flux of the dinoflagellate Gymnodinium instriatum . Ecotoxicology 25, 248–252 (2016). https://doi.org/10.1007/s10646-015-1564-z

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