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Bone Marrow Cells Enhance Liver Regeneration After Massive Hepatectomy in Mice

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Abstract

Background

Recent evidence indicates that transplanted autologous bone marrow cells (BMCs) can be converted into functional liver cells. BMC therapy can improve hepatic function and increase the potential for liver regeneration in patients with serious liver damage. We investigated whether BMC therapy influenced liver regeneration after massive hepatectomy in mice.

Methods

Male C57/BL6 mice underwent 70 % hepatectomy, followed by injection of BMCs via the portal vein (PV group), BMCs via the tail vein (IV group), or saline via the portal vein (control group). Analysis of serum enzyme levels and liver histology was performed on postoperative days (POD) 1, 3, and 5.

Results

Compared with the control group, the rate of liver regeneration on POD 3 and 5 was significantly higher in the PV group, but not in the IV group. Examination of the mitotic index and Ki-67 labeling index revealed that the increased liver regeneration resulted from stimulation of DNA synthesis. On POD 3, the serum levels of interleukin (IL)-6 and hepatocyte growth factor (HGF) were significantly higher and the expression of IL-6 and HGF mRNA in the remnant liver tended to be higher in the PV group than in the control group. Histological examination showed BMCs in the liver of the PV group, as well as conversion of BMCs into liver cells.

Conclusions

Our findings indicate that the injection of BMCs via the portal vein, but not the injection of BMCs via the tail, enhances liver regeneration after massive hepatectomy in mice.

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Authors and Affiliations

  1. Department of Surgery, Hirakata Hospital, Kansai Medical University, 2-3-1 Shinmachi, Hirakata, Osaka, 573-1191, Japan

    Masaki Kaibori, Morihiko Ishizaki, Kosuke Matsui, Yoshito Tanaka, Masaharu Ohishi, Yoshiro Araki, Katsuji Tokuhara, Tadayoshi Okumura & A-Hon Kwon

  2. Division of Clinical Pathology, Toyooka Hospital, Toyooka, Hyogo, 668-8501, Japan

    Yasushi Adachi

  3. Department of Pediatrics, Kansai Medical University, Hirakata, Osaka, 573-1191, Japan

    Tomohiko Shimo

  4. Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, 525-8577, Japan

    Mikio Nishizawa

Authors
  1. Masaki Kaibori
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  2. Yasushi Adachi
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  3. Tomohiko Shimo
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  4. Morihiko Ishizaki
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  5. Kosuke Matsui
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  6. Yoshito Tanaka
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  7. Masaharu Ohishi
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  8. Yoshiro Araki
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  9. Katsuji Tokuhara
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  10. Tadayoshi Okumura
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  11. Mikio Nishizawa
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  12. A-Hon Kwon
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Correspondence to Masaki Kaibori.

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Kaibori, M., Adachi, Y., Shimo, T. et al. Bone Marrow Cells Enhance Liver Regeneration After Massive Hepatectomy in Mice. Dig Dis Sci 59, 1484–1489 (2014). https://doi.org/10.1007/s10620-014-3032-7

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  • DOI: https://doi.org/10.1007/s10620-014-3032-7

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