Skip to main content

Advertisement

SpringerLink
Log in

Isolation and characterization of polymorphic microsatellite markers for the endangered tree Handeliodendron bodinieri (Sapindaceae)

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

Nine polymorphic microsatellite loci were developed and characterized for Handeliodendron bodinieri, an endangered tree endemic to karst forest in southwest China. Each locus displayed between 4 and 11 alleles. The observed heterozygosity ranged from 0.345 to 0.931 and expected heterozygosity varied from 0.414 to 0.824. The combined probabilities of paternity exclusion over all loci were 0.931 for P r(Ex1) and 0.993 for P r(Ex2), respectively. These loci represent a powerful tool in investigating gene flow and population structure in natural populations of H. bodinieri.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Fu LK, Jin JM (1992) China plant red data book-rare and endangered plant. Science Press, Beijing

    Google Scholar 

  • Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106

    Article  PubMed  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (Version 3.4): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the www for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, USA, pp 365–386

    Google Scholar 

  • Slate J, Marshall T, Pemberton J (2000) A retrospective assessment of the accuracy of the paternity inference program CERVUS. Mol Ecol 9:801–808

    Article  PubMed  CAS  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370

    Article  Google Scholar 

  • Xiong ZB, Ran JC, Tan CJ et al (2003) The seed ecological characteristics of endangered Handeliodendron bodinierei. Acta Ecol Sin 23:820–825

    Google Scholar 

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This project was supported by National Natural Science Foundation of China (30470185) to M.K.

Author information

Authors and Affiliations

  1. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, 430074, China

    Jing Wang, Puxin Gao & Ming Kang

Authors
  1. Jing Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Puxin Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ming Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ming Kang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, J., Gao, P. & Kang, M. Isolation and characterization of polymorphic microsatellite markers for the endangered tree Handeliodendron bodinieri (Sapindaceae). Conserv Genet 9, 727–729 (2008). https://doi.org/10.1007/s10592-007-9363-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-007-9363-6

Keywords

Search

Navigation