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Bioremediation using Novosphingobium strain DY4 for 2,4-dichlorophenoxyacetic acid-contaminated soil and impact on microbial community structure

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Abstract

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is commonly used for weed control. The ubiquity of 2,4-D has gained increasing environmental concerns. Biodegradation is an attractive way to clean up 2,4-D in contaminated soil. However, information on the bioaugmentation trial for remediating contaminated soil is still very limited. The impact of bioaugmentation using 2,4-D-degraders on soil microbial community remains unknown. The present study investigated the bioremediation potential of a novel degrader (strain DY4) for heavily 2,4-D-polluted soil and its bioaugmentation impact on microbial community structure. The strain DY4 was classified as a Novosphingobium species within class Alphaproteobacteria and harbored 2,4-D-degrading TfdAα gene. More than 50 and 95 % of the herbicide could be dissipated in bioaugmented soil (amended with 200 mg/kg 2,4-D) respectively in 3–4 and 5–7 days after inoculation of Novosphingobium strain DY4. A significant growth of the strain DY4 was observed in bioaugmented soil with the biodegradation of 2,4-D. Moreover, herbicide application significantly altered soil bacterial community structure but bioaumentation using the strain DY4 showed a relatively weak impact.

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Acknowledgments

This work was financially supported by special fund of State Key Joint Laboratory of Environment Simulation and Pollution Control (No. 14Y02ESPCP).

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Authors and Affiliations

  1. State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China

    Yu Dai, Qun Zhao & Shuguang Xie

  2. Yuanpei College, Peking University, Beijing, 100871, China

    Ningning Li

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  1. Yu Dai
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  3. Qun Zhao
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  4. Shuguang Xie
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Correspondence to Shuguang Xie.

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Dai, Y., Li, N., Zhao, Q. et al. Bioremediation using Novosphingobium strain DY4 for 2,4-dichlorophenoxyacetic acid-contaminated soil and impact on microbial community structure. Biodegradation 26, 161–170 (2015). https://doi.org/10.1007/s10532-015-9724-7

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