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Effect of N-Acetylcysteine on Lipopolysaccharide-Induced Uveitis in Rats

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Abstract

Purpose

In this study we investigated the in vivo effect of N-acetylcysteine (NAC) on lipopolysaccharide (LPS)-induced uveitis in rats.

Methods

To induce uveitis, LPS (100 µg) was injected into subcutaneous tissue of Wistar rats (170–190 g). NAC was injected intraperitoneally. Intracameral levels of protein, cells, nitrite, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were determined by spectrophotometry, hemocytometry, and enzyme-linked immunosorbent assay. Expression of TNF-α, IL-6, endothelial leukocyte adhesion molecule-1 (E-selectin), intercellular adhesion molecule-1 (ICAM-1), and inducible nitric oxide synthase (iNOS) mRNA was examined by real-time polymerase chain reaction.

Results

LPS injection elevated intracameral protein and cells, and the elevation was inhibited by NAC. LPS injection induced expression of TNF-α, IL-6, E-selectin, and ICAM-1 mRNA in the iris/ciliary body at 3 h, and iNOS mRNA at 6 h. The LPS-induced elevation of the mRNA levels was inhibited by NAC. NAC inhibited LPS-induced intracameral elevation of TNF-α, IL-6, and nitrite.

Conclusion

NAC decreased LPS-induced uveitis in vivo by reducing the expression of proinflammatory cytokines and adhesion molecules. Jpn J Ophthalmol 2007;51:14–20 © Japanese Ophthalmological Society 2007

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Authors and Affiliations

  1. Department of Ophthalmology, School of Medicine, University of Toyama, Toyama, Japan

    Xue-Yun Zhang, Seiji Hayasaka, Yoriko Hayasaka, Hu-Shan Cui & Zai-Long Chi

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  1. Xue-Yun Zhang
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  2. Seiji Hayasaka
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  3. Yoriko Hayasaka
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  4. Hu-Shan Cui
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  5. Zai-Long Chi
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Correspondence to Xue-Yun Zhang.

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Zhang, XY., Hayasaka, S., Hayasaka, Y. et al. Effect of N-Acetylcysteine on Lipopolysaccharide-Induced Uveitis in Rats. Jpn J Ophthalmol 51, 14–20 (2007). https://doi.org/10.1007/s10384-006-0382-5

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  • DOI: https://doi.org/10.1007/s10384-006-0382-5

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