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Mitochondrial proteomes of porcine kidney cortex and medulla: foundation for translational proteomics

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Clinical and Experimental Nephrology Aims and scope Submit manuscript

Abstract

Background

Emerging evidence has linked mitochondrial dysfunction to the pathogenesis of many renal disorders, including acute kidney injury, sepsis and even chronic kidney disease. Proteomics is a powerful tool in elucidating the role of mitochondria in renal pathologies. Since the pig is increasingly recognized as a major mammalian model for translational research, the lack of physiological proteome data of large mammals prompted us to examine renal mitochondrial proteome in porcine kidney cortex and medulla

Methods

Kidneys were obtained from six healthy pigs. Mitochondria from cortex and medulla were isolated using differential centrifugation and proteome maps of cortical and medullar mitochondria were constructed using two-dimensional gel electrophoresis (2DE). Protein spots with significant difference between mitochondrial fraction of renal cortex and medulla were identified by mass spectrometry.

Results

Proteomic analysis identified 81 protein spots. Of these spots, 41 mitochondrial proteins were statistically different between renal cortex and medulla (p < 0.05). Protein spots containing enzymes of beta oxidation, amino acid metabolism, and gluconeogenesis were predominant in kidney cortex mitochondria. Spots containing tricarboxylic acid cycle enzymes and electron transport system proteins, proteins maintaining metabolite transport and mitochondrial translation were more abundant in medullar mitochondria.

Conclusion

This study provides the first proteomic profile of porcine kidney cortex and medullar mitochondrial proteome. Different protein expression pattern reflects divergent functional metabolic role of mitochondria in various kidney compartments. Our study could serve as a useful reference for further porcine experiments investigating renal mitochondrial physiology under various pathological states.

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Abbreviations

2DE:

Two-dimensional electrophoresis

KM:

Kidney medulla

KC:

Kidney cortex

TCA:

Tricarboxylic acid cycle

ETS:

Electron transport system

ROS:

Reactive oxygen species

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Acknowledgments

This work was supported by the Research Project No. MSM0021620819 “Replacement of and Support to Some Vital Organs”, by the Charles University Research Fund (project number P36) by the project ED2.1.00/03.0076 by the European Regional Development Fund, and the Specific Student Research Project no. 260175/2015 of the Charles University in Prague.

Conflict of interest

All the authors have declared no competing interest.

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Authors and Affiliations

  1. Faculty of Medicine in Plzen, Biomedical Center, Charles University in Prague, alej Svobody, 1655/76, Plzen, Czech Republic

    Zdenek Tuma, Jitka Kuncova, Jan Mares & Martin Matejovic

  2. Department of Physiology, Charles University Medical School, Plzen, Czech Republic

    Jitka Kuncova

  3. Department of Internal Medicine I, Charles University Medical School and Teaching Hospital, Plzen, Czech Republic

    Jan Mares & Martin Matejovic

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  1. Zdenek Tuma
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  2. Jitka Kuncova
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Correspondence to Zdenek Tuma.

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Tuma, Z., Kuncova, J., Mares, J. et al. Mitochondrial proteomes of porcine kidney cortex and medulla: foundation for translational proteomics. Clin Exp Nephrol 20, 39–49 (2016). https://doi.org/10.1007/s10157-015-1135-x

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  • DOI: https://doi.org/10.1007/s10157-015-1135-x

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