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Isolation, Expression, and Characterization of Blue Light Receptor AUREOCHROME Gene From Saccharina japonica (Laminariales, Phaeophyceae)

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Abstract

Photosynthetic stramenopile have chloroplasts of secondary endosymbiotic origin and are significant as aquatic primary productivity and biomass production. In marine environments, many photosynthetic stramenopiles utilize blue light to regulate growth, development, and organelle movement. Aureochrome (AUREO) is a new type blue light photoreceptor specific in photosynthetic stramenopiles. Previously, several AUREO orthologs were reported in genomes of stramenopile members, but the full-length cDNA sequences were completed only in Vaucheria frigida (Xanthophyceae), Fucus distichus (Phaeophyceae), and Ochromonas danica (Chrysophyceae). In this study, the full-length cDNA of AUREO from Saccharina japonica (designated as SjAUREO) was isolated based on homologous cloning and the rapid amplification of cDNA ends (RACE). It characterized by the full length of 1,013 bp with an open reading frame of 612 bp, which encoded a polypeptide of 203 amino acids with predicted molecular weight of 23.08 kDa and theoretical isoelectric point of 7.63. The deduced amino acid sequence of SjAUREO contained one N-terminal basic region/leucine zipper (bZIP) transcription regulation domain and a single light-, oxygen-, or voltage-sensitive (LOV) domain near the C-terminus. Homologous analysis showed that SjAUREO shared 40–92 % similarities with those of other photosynthetic stramenopiles. Phylogenetic analysis revealed close phylogenetic affinity between SjAUREO and AUREO4 of brown alga Ectocarpus siliculosus. Real-time PCR detection revealed that the SjAUREO transcription was markedly increased under BL exposure and dramatically upregulated in the 1-month juvenile sporophyte than those in the 2 and 3-month materials, which indirectly reflected the SjAUREO associated with the BL-mediated photomorphogenesis during the growth and early development of juvenile sporophytes. In vitro expression showed one distinct band existed at ∼27 kDa, and western blot detection proved that it was positive to the anti-His antibody with high specificity. Our results enriched the knowledge of AUREO properties in S. japonica and provided clues to explore the mechanisms underlying diverse physiological responses mediated by BL photoreceptors AUREO in the photosynthetic stramenopiles.

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Acknowledgment

This research was supported by National Natural Science Foundation of China (No. 40976085) and Shandong Agriculture Breeding Engineering Biological Resources Innovation of Research Project. We sincerely thank Drs. Hironao Kataoka and Fumio Takahashi for their valuable comments and critical reading of the manuscript. Thanks are either due to Lin Xiao, Jin Zhao, and Ge Liu for their help with the experiments. The authors acknowledged the anonymous reviewers for the critical comments and suggestions for the manuscript.

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  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Yunyan Deng, Jianting Yao, Gang Fu, Hui Guo & Delin Duan

  2. University of the Chinese Academy of Sciences, Beijing, 100049, China

    Hui Guo

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  1. Yunyan Deng
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Deng, Y., Yao, J., Fu, G. et al. Isolation, Expression, and Characterization of Blue Light Receptor AUREOCHROME Gene From Saccharina japonica (Laminariales, Phaeophyceae). Mar Biotechnol 16, 135–143 (2014). https://doi.org/10.1007/s10126-013-9539-7

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