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Adipose Triglyceride Lipase (ATGL) Clone, Expression Pattern, and Regulation by Different Lipid Sources and Lipid Levels in Large Yellow Croaker (Pseudosciaena crocea R.)

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Abstract

Adipose triglyceride lipase (ATGL) was recently identified as a triglyceride (TG)-specific lipase. In this study, we first obtained from large yellow croaker fish a 1,820-bp (GenBank ID: HQ916211) ATGL cDNA fragment with a 141-bp 5′UTR, a 1,485-bp open reading frame, and a 194-bp 3′UTR. The predicted fish ATGL had 494 amino acids (GenBank ID: ADY89608) and a calculated molecular weight of 55.1 kDa. ATGL was highly expressed in liver and, to a lesser degree, in heart, muscle, and abdominal fat. ATGL gene expression was high at 4.5 g and then decreased at 157.9 g and increased again at 474.2 g. The effects of lipid levels and lipid sources on ATGL expression in vivo were also investigated. A high-lipid diet decreased ATGL expression in fish significantly (P < 0.01). Fish in soybean oil group exhibited significantly lower ATGL expression than fish in the fish oil and beef tallow groups (P < 0.01). These data enhance our understanding of ATGL in fish lipid metabolism.

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Abbreviations

ATGL:

Adipose triglyceride lipase

RACE:

Rapid amplification of cDNA ends

TG:

Triglyceride

DG:

Diglyceride

MUFA:

Monounsaturated fatty acid

SGR:

Specific growth rate

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC, grant # 30901104) and the China Postdoctoral Science Foundation (grant # 2009046138).

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Authors and Affiliations

  1. Institute of Feed Science, Zhejiang University, No. 388 Yuhangtang Road, Hangzhou, People’s Republic of China

    Xinxia Wang, Yizhen Wang & Yongjin Li

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  1. Xinxia Wang
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  2. Yizhen Wang
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  3. Yongjin Li
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Correspondence to Yizhen Wang.

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Wang, X., Wang, Y. & Li, Y. Adipose Triglyceride Lipase (ATGL) Clone, Expression Pattern, and Regulation by Different Lipid Sources and Lipid Levels in Large Yellow Croaker (Pseudosciaena crocea R.). Mar Biotechnol 15, 197–205 (2013). https://doi.org/10.1007/s10126-012-9477-9

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  • DOI: https://doi.org/10.1007/s10126-012-9477-9

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