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Establishment, Characterization, and Virus Susceptibility of a New Marine Cell Line from Red Spotted Grouper (Epinephelus akaara)

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Abstract

A marine fish cell line from the snout of red spotted grouper Epinephelus akaara, a protogynous hermaphrodite, was established, characterized, and subcultured with more than 60 passages. The grouper snout cell line (GSC) cells multiplied well in Dulbecco’s modified Eagle’s medium (DMEM) medium supplemented with 10% fetal bovine serum. The optimal growth temperature was 25°C, and morphologically the cells were fibroblastic. Chromosome analysis revealed that the GSC cell line has a normal diploid karyotype with \( 2n = 8st + 40t \). A virus titration study indicated that the cells were susceptible to turbot Scophthalmus Maximus rhabdovirus (SMRV) (108.5 TCID50 ml−1), while the viral titer of frog Rana grylio virus 9807 (RGV9807) reached 103.5 TCID50 ml−1. The infection was confirmed by cytopathic effect (CPE), immunofluorescence, and electron microscopy experiments, which detected the viral particles in the cytoplasm of virus-infected cells, respectively. Further, significant fluorescent signals were observed when the GSC cells were transfected with pEGFP vector DNA, indicating their potential utility for transgenic and genetic manipulation studies.

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Acknowledgments

This study was supported by grants from the National Major Basic Research Program (2004CB117403), the National 863 High Technology Research Foundation of China, the National Natural Science Foundation of China (30671616, U0631008), and the Project of Chinese Academy of Sciences (KSCX2-YW-N-021).

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Authors and Affiliations

  1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate school of the Chinese Academy of Sciences, Wuhan, 430072, China

    Guang-Zhou Zhou, Zheng-Qiu Li, Xiu-Ping Yuan & Qi-Ya Zhang

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  1. Guang-Zhou Zhou
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  2. Zheng-Qiu Li
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  3. Xiu-Ping Yuan
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  4. Qi-Ya Zhang
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Correspondence to Qi-Ya Zhang.

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Zhou, GZ., Li, ZQ., Yuan, XP. et al. Establishment, Characterization, and Virus Susceptibility of a New Marine Cell Line from Red Spotted Grouper (Epinephelus akaara). Mar Biotechnol 9, 370–376 (2007). https://doi.org/10.1007/s10126-006-7165-3

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