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Study of Genetic Diversity of Orange-Spotted Grouper, Epinephelus coioides, from Thailand and Indonesia Using Microsatellite Markers

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Abstract

Genetic diversity of Epinephelus coioides (Hamilton, 1822), which inhabits coastal reefs from the western Indian Ocean to the western Pacific Ocean, was studied based on four polymorphic microsatellite loci. Two hundred and fifty individuals were collected from two locations in Thailand (Nakornsrithammarat-N and Trang-T) and four in Indonesia (Sibolga-S, Lampung-L, Jepara-J, and Flores-F). The genetic variation of E. coioides was relatively low; the observed heterozygosities (Ho) ranged between 0.36 (F) and 0.55 (N). The average number of alleles/locus was between 3.57 (L) and 5.09 (J). Genotypic distribution for most population pairs was significantly different after Bonferroni correction (P < 0.0024) except for J and F. Population structuring was significant (F ST = 0.074). The genetic distances between populations ranged between 0.016 (L and N) to 0.086 (F and S). Mantel's test showed no correlation between genetic distance and geographical distance. The NJ tree clearly separated N from the others which comprised two subgroups, T-S and L-J-F.

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Acknowledgments

We would like to thank the Thailand Research Fund for funding this research through the project entitled “Application of Genetics and Biotechnology for Sustainable Development of Aquaculture” (Senior Research Scholar 2004) awarded to Uthairat Na-Nakorn. We thank Dr. Wongpathom Kamonrat, Department of Fisheries, Ministry of Agriculture and Cooperative, Thailand and Srijanya Sukmanomon, Department of Aquaculture, Faculty of Fisheries, Kasetsart University for their help in data analyses. Sincere appreciation is extended to Professor Fred Allendorf, University of Montana, USA, and the anonymous referees for their critical comments on the manuscript.

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Authors and Affiliations

  1. National Sea Farming Development Center, Lampung, 35454, Indonesia

    Suci Antoro

  2. Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand

    Uthairat Na-Nakorn

  3. Department of Aquaculture, Faculty of Science and Fisheries Technology, Rajamangala Institute of Technology, Trang, 92150, Thailand

    Worawut Koedprang

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  1. Suci Antoro
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  2. Uthairat Na-Nakorn
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Correspondence to Uthairat Na-Nakorn.

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Antoro, S., Na-Nakorn, U. & Koedprang, W. Study of Genetic Diversity of Orange-Spotted Grouper, Epinephelus coioides, from Thailand and Indonesia Using Microsatellite Markers. Mar Biotechnol 8, 17–26 (2006). https://doi.org/10.1007/s10126-005-5026-0

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