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Anti-inflammatory activity of ethanolic extract from skipjack tuna (Katsuwonus pelamis) heart in LPS-induced RAW 264.7 cells and mouse ear edema model

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Abstract

This study investigated the anti-inflammatory activity of the ethanolic extract (THEE) obtained from the heart of skipjack tuna using lipopolysaccharide (LPS)-induced RAW 264.7 cells. THEE markedly suppressed the production of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and IL-1β in a dose-dependent manner. In addition, THEE decreased the expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappa B p65 (NF-κB p65), and mitogen-activated protein kinases (MAPKs) including phosphorylated c-Jun NH2-terminal kinase (p-JNK), phosphorylated extracellular signal-related kinase (p-ERK), and p-p38 proteins. Moreover, THEE orally treated at doses of 50, 100, and 250 mg/kg inhibited the croton oil-induced edema formation and the reduction of the epidermal/dermal thickness and the mast cell numbers was observed in histological analysis. There were no mortalities occurred in mice administered THEE at 5,000 mg/kg body weight. Taken together, these results indicate that THEE exerts the anti-inflammatory activities via inhibition of NF-κB and MAPKs activation.

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Authors and Affiliations

  1. Institute of Fisheries Sciences, Pukyong National University, Busan, 46041, Korea

    Koth-Bong-Woo-Ri Kim

  2. Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan, 48513, Korea

    Bo-Kyeong Kang, Min-Ji Kim & Dong-Hyun Ahn

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  1. Bo-Kyeong Kang
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  2. Min-Ji Kim
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Correspondence to Dong-Hyun Ahn.

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Kang, BK., Kim, MJ., Kim, KBWR. et al. Anti-inflammatory activity of ethanolic extract from skipjack tuna (Katsuwonus pelamis) heart in LPS-induced RAW 264.7 cells and mouse ear edema model. Food Sci Biotechnol 25, 847–854 (2016). https://doi.org/10.1007/s10068-016-0140-5

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  • DOI: https://doi.org/10.1007/s10068-016-0140-5

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