Abstract
Sulfated polysaccharides isolated from Enteromorpha prolifera and fractionated using ion exchange and gel permeation chromatography were investigated to determine their molecular characteristics and biological activities. The crude and fractionated polysaccharides (F1, F2, and F3) consisted mostly of carbohydrates (50.8–62.5%), sulfates (14.5–18.8%), and uronic acid (13.8–16.6%) with different levels of monosaccharides, including rhamnose (57.1–87.6%), glucose (3.6-39.1%), and xylose (2.4–8.8%). The protein content ranged from 1.0 to 13.9%, and after the fractionation, most proteins were included in the F2 fraction (11.3%). The crude, F1, F2, and F3 exhibited the molecular weights (Mw) of 37–1,281×103 g/mol. The polysaccharides had no significant direct cytotoxicity to cancer cells AGS or DLD- 1. On the other hand, the F1 and F2 stimulated Raw 264.7 cells to produce a considerable amount of nitric oxide (NO). The investigation of F2 subfractions failed to reveal a specific subfraction more significantly affecting the NO production, thus requiring further systematic elucidation of their structural characteristics.
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References
Morand P, Briand X. Excessive growth of macroalgae: A symptom of environmental disturbance. Bot. Mar. 39: 491–516 (1996)
Zhang HJ, Mao WJ, Fang F, Li HY, Sun HH, Chen Y, Qi XH. Chemical characteristics and anticoagulant activities of a sulfated polysaccharide and its fragments from Monostroma latissimum. Carbohyd. Polym. 71: 428–434 (2008)
Lahaye M, Vigouroux J, Thibault JE. Endo-β-1,4-D-galactanase from Aspergillus niger var. aculeatus: Purification and some properties. Carbohyd. Polym. 15: 431–444 (1991)
Lahaye M, Brunel M, Bonnin E. Fine chemical structure analysis of oligosaccharides produced by an ulvan-lyase degradation of the water-soluble cell-wall polysaccharides from Ulva sp. (Ulvales, Chlorophyta). Carbohyd. Res. 304: 325–333 (1997)
Mabeau S, Fleurence J. Seaweed in food products: Biochemical and nutritional aspects. Trends Food Sci. Tech. 4: 103–107 (1993)
Fleurence J. Seaweed proteins: Biochemical, nutritional aspects, and potential uses. Trends Food Sci. Tech. 10: 25–28 (1999)
Ray B, Lahaye M. Cell-wall polysaccharides from the marine green alga Ulva “rigida” (Ulvales, Chlorophyta). Chemical structure of ulvan. Carbohyd. Res. 274: 313–318 (1995
Lahaye M, Ray B. Cell-wall polysaccharides from the marine green alga Ulva “rigida” (Ulvales, Chlorophyta) — NMR analysis of ulvan oligosaccharides. Carbohyd. Res. 283: 161–173 (1996
Ray B. Polysaccharides from Enteromorpha compressa: Isolation, purification, and structural features. Carbohyd. Polym. 66: 408–416 (2006)
Robic A, Mouro CR, Sassi JF, Lerat Y, Lahaye M. Structure and interactions of ulvan in the cell wall of the marine green algae Ulva rotundata (Ulvales, Chlorophyceae). Carbohyd. Polym. 77: 206–216 (2009)
Cassolato JEF, Noseda MD, Pujol CA, Pellizzari FM, Damonte EB, Duarte MER. Chemical structure and antiviral activity of the sulfated heterorhamnan isolated from the green seaweed Gayralia oxysperma. Carbohyd. Res. 343: 3085–3095 (2008)
Chattopadhyay K, Mandal P, Lerouge P, Driouich A, Ghosal P, Ray B. Sulphated polysaccharides from Indian samples of Enteromorpha compressa (Ulvales, Chlorophyta): Isolation and structural features. Food Chem. 104: 928–935 (2007)
Kaeffer B, Benard C, Lahaye M, Blottiere HM, Cherbut C. Biological properties of ulvan, a new source of green seaweed sulfated polysaccharides, on cultured normal and cancerous colonic epithelial cells. Planta Med. 65: 527–531 (1999)
Ivanova V, Rouseva R, Kolarova M, Serkedjieva J, Rachev R, Manolova N. Isolation of a polysaccharide with antiviral effect from Ulva lactuca. Prep. Biochem. 24: 83–97 (1994)
Pengzhan Y, Quanbin Z, Ning L, Zuhong X, Yanmei W, Zhien L. Polysaccharides from Ulva pertusa (Chlorophyta) and preliminary studies on their antihyperlipidemia activity. J. Appl. Phycol. 15: 21–27 (2003)
Qi H, Zhang Q, Zhao T, Chen R, Zhang H, Niu X, Li Z. Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro. Int. J. Biol. Macromol. 37: 195–199 (2005)
Pereira MS, Melo FR, Mourao PAS. Is there a correlation between structure and anticoagulant action of sulfated galactans and sulfated fucans? Glycobiology 12: 573–580 (2002)
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356 (1956)
Dodgson KS, Price RG. A note on the determination of the ester sulphate content of sulphated polysaccharides. Biochem. J. 84: 106–110 (1962)
Filisetti-Cozzi TMCC, Carpita NC. Measurement of uronic acids without interference from neutral sugars. Anal. Biochem. 197: 157–162 (1991)
Green LC, Wanger DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal. Biochem. 126: 131–136 (1982)
Lahaye M, Cimadevilla EAC, Kuhlenkamp R, Quemener B, Lognone V, Dion P. Chemical composition and 13C NMR spectroscopic characterisation of ulvans from Ulva (Ulvales, Chlorophyta). J. Appl. Phycol. 11: 1–7 (1999)
Mao W, Li H, Li F, Zhang H, Qi X, Sun H, Chen Y, Guo S. Chemical characteristic and anticoagulant activity of the sulfated polysaccharide isolated from Monostroma latissimum (Chlorophyta). Int. J. Biol. Macromol. 44: 70–74 (2009)
Yang C, Chung D, Shin IS, Lee HY, Kim JC, Lee YJ, You SG. Effects of molecular weight and hydrolysis conditions on anticancer activity of fucoidans from sporophyll of Undaria pinnatifida. Int. J. Biol. Macromol. 43: 433–437 (2008)
Leiro JM, Castro R, Arranz JA, Lamas J. Immunomodulating activities of acidic sulphated polysaccharides obtained from the seaweed Ulva rigida C. Agardh. Int. Immunopharmacol. 7: 879–888 (2007)
You SG, Lim ST. Molecular characterization of corn starch using an aqueous HPSEC-MALLS-RI system under various dissolution and analytical conditions. Cereal Chem. 77: 303–308 (2000)
Kurashige S, Akuzawa Y, Endo F. Effects of Lentinus edodes, Grifola frondosa, and Pleurotus ostreatus administration on cancer outbreak, and activities of macrophages and lymphocytes in mice treated with a carcinogen, N-butyl-N-butanolnitrosoamine. Immunopharm. Immunot. 19: 175–183 (1997)
Jiao L, Li X, Li T, Jiang P, Zhang L, Wu M, Zhang L. Characterization and anti-tumor activity of alkali-extracted polysaccharide from Enteromorpha intestinalis. Int. Immunopharmacol. 9: 324–329 (2009)
Taylor PR, Brown GD, Reid DM, Willment JA, Pomares LM, Gordon S, Wong SYC. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J. Immunol. 169: 3876–3882 (2002)
Schepetkin IA, Xie G, Kirpotina LN, Klein RA, Jutila MA, Quinn MT. Macrophage immunomodulatory activity of polysaccharides isolated from Opuntia polyacantha. Int. Immunopharmacol. 8: 1455–1466 (2008)
Kim GY, Han MG, Song YS, Shin BC, Shin YI, Lee HJ, Moon DO, Lee CM, Kwak JY, Bae YS, Lee JD. Park YM. Proteoglycan isolated from Phellinus linteus induces toll-like receptors 2- and 4- mediated maturation of murine dendritic cells via activation of ERK, p38, and NF-kappa B. Biol. Pharm. Bull. 27: 1656–1662 (2004)
Sarangi I, Ghosh D, Bhutia SK, Mallick SK, Maiti TK. Anti-tumor and immunomodulating effects of Pleurotus ostreatus myceliaderived proteoglycans. Int. Immunopharmacol. 6: 1287–1297 (2006)
Shah S, Ghosh D, Mallick SK, Sarangi I, Bhutia SK, Banerje I, Maiti S, Maiti TK. NO reducing and antitumor activities of watersoluble proteoglycan isolated from the fruiting bodies of culinarymedicinal oyster mushroom Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae). Int. J. Med. Mushrooms 9: 123–138 (2007)
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Cho, M., Yang, C., Kim, S.M. et al. Molecular characterization and biological activities of watersoluble sulfated polysaccharides from Enteromorpha prolifera . Food Sci Biotechnol 19, 525–533 (2010). https://doi.org/10.1007/s10068-010-0073-3
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DOI: https://doi.org/10.1007/s10068-010-0073-3