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A novel small animal extracorporeal circulation model for studying pathophysiology of cardiopulmonary bypass

  • Original Article
  • Cardiopulmonary Bypass
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Abstract

Extracorporeal circulation (ECC) is indispensable for cardiac surgery. Despite the fact that ECCcauses damage to blood components and is non-physiologic, its pathophysiology has not been fully elucidated. This is because difficulty in clinical research and animal experiments keeps the knowledge insufficient. Therefore, it is desirable to have a miniature ECC model for small animals, which enables repetitive experiments, to study the mechanism of pathophysiological changes during ECC. We developed a miniature ECC system and applied it to the rat. We measured changes in hemodynamics, blood gases and hemoglobin (Hb) concentration, serum cytokines (TNF-α, IL-6, IL-10), biochemical markers (LDH, AST, ALT), and the wet-to-dry weight (W/D) ratio of the lung for assessing whether the rat ECC model is comparable to the human ECC. The ECC system consisted of a membranous oxygenator (polypropylene, 0.03 m2), tubing line (polyvinyl chloride), and roller pump. Priming volume of this system is only 8 ml. Rats (400–450 g) were divided into the SHAM group (n = 7) and the ECC group (n = 7). Blood samples were collected before, 60 and 120 min after initiation of ECC. During ECC, blood pressure and Hb were maintained around 80 mmHg and 10 g/dL, respectively. The levels of the inflammatory and biochemical markers and the W/D ratio were significantly elevated in the ECC group, indicating some organ damages and systemic inflammatory responses during ECC. We successfully established the ECC for the rat. This miniature ECC model could be a useful approach for studying the mechanism of pathophysiology during ECC and basic assessment of the ECC devices.

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The authors have no conflict of interest directly relevant to the content of this article.

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Authors and Affiliations

  1. Department of Artificial Organs, National Cerebral and Cardiovascular Center Research Institute, 5-7-1, Fujishiro-dai, Suita, Osaka, 565-8565, Japan

    Yutaka Fujii, Yoshiaki Takewa & Eisuke Tatsumi

  2. Department of Cardiac Physiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1, Fujishiro-dai, Suita, Osaka, 565-8565, Japan

    Mikiyasu Shirai

  3. Department of Clinical Engineering Faculty of Health Sciences, Hiroshima International University, 555-36, Kurose-gakuendai, Higashi-hiroshima, Hiroshima, 739-2631, Japan

    Shuji Inamori

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  1. Yutaka Fujii
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  2. Mikiyasu Shirai
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  3. Shuji Inamori
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  4. Yoshiaki Takewa
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  5. Eisuke Tatsumi
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Correspondence to Yutaka Fujii.

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Fujii, Y., Shirai, M., Inamori, S. et al. A novel small animal extracorporeal circulation model for studying pathophysiology of cardiopulmonary bypass. J Artif Organs 18, 35–39 (2015). https://doi.org/10.1007/s10047-014-0804-y

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  • DOI: https://doi.org/10.1007/s10047-014-0804-y

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