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Efficient transfection method using deacylated polyethylenimine-coated magnetic nanoparticles

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Abstract

Low efficiencies of nonviral gene vectors, such as transfection reagent, limit their utility in gene therapy. To overcome this disadvantage, we report on the preparation and properties of magnetic nanoparticles [diameter (d) = 121.32 ± 27.36 nm] positively charged by cationic polymer deacylated polyethylenimine (PEI max), which boosts gene delivery efficiency compare with polyethylenimine (PEI), and their use for the forced expression of plasmid delivery by application of a magnetic field. Magnetic nanoparticles were coated with PEI max, which enabled their electrostatic interaction with negatively charged molecules such as plasmid. We successfully transfected 81.1 ± 4.0% of the cells using PEI max-coated magnetic nanoparticles (PEI max-nanoparticles). Along with their superior properties as a DNA delivery vehicle, PEI max-nanoparticles offer to deliver various DNA formulations in addition to traditional methods. Furthermore, efficiency of the gene transfer was not inhibited in the presence of serum in the cells. PEI max-nanoparticles may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.

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Acknowledgments

We express our sincere thanks to Koichiro Nishino (Department of Reproductive Biology, National Institute for Child Health and Development) for pCAGGS-EGFP. This study was supported by a Grant-in-Aid for the Global COE Program, Science for Future Molecular Systems from the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT).

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Authors and Affiliations

  1. Innovative Integration between Medicine and Engineering Based on Information Communications Technology, Yokohama National University Global COE Program, Yokohama, Japan

    Daisuke Kami

  2. Laboratory for Medical Engineering, Division of Materials and Chemical Engineering, Yokohama National University, 79-1 Tokiwadai, Hodogaya-ku, Yokohama, 240-8501, Japan

    Shogo Takeda & Masatoshi Watanabe

  3. Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, Japan

    Daisuke Kami, Hatsune Makino, Masashi Toyoda & Akihiro Umezawa

  4. Vascular Medicine, Research Team for Geriatric Medicine, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan

    Daisuke Kami, Masashi Toyoda, Yoko Itakura & Satoshi Gojo

  5. Division of Therapeutic Strategy for Heart Failure, Department of Cardio-Thoracic Surgery, The University of Tokyo, Tokyo, Japan

    Satoshi Gojo & Shunei Kyo

Authors
  1. Daisuke Kami
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  2. Shogo Takeda
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  3. Hatsune Makino
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  4. Masashi Toyoda
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  5. Yoko Itakura
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  6. Satoshi Gojo
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  7. Shunei Kyo
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  8. Akihiro Umezawa
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  9. Masatoshi Watanabe
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Correspondence to Masashi Toyoda or Masatoshi Watanabe.

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Kami, D., Takeda, S., Makino, H. et al. Efficient transfection method using deacylated polyethylenimine-coated magnetic nanoparticles. J Artif Organs 14, 215–222 (2011). https://doi.org/10.1007/s10047-011-0568-6

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  • DOI: https://doi.org/10.1007/s10047-011-0568-6

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