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ATF-2 immunoreactivity in post-mitotic and terminally differentiated human odontoblasts

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Abstract

Activating transcription factor 2 (ATF-2/CRE-BP1; cAMP-responsive element binding protein 1) is a member of nuclear transcription factor activator protein-1 (AP-1) family. AP-1 regulates cellular processes including growth, proliferation, differentiation and apoptosis. However, biological relationship of cellular process to each member of the AP-1 family is not clear yet. The objective of the present study was to compare the ATF-2 immunoreactivity in the post-mitotic and terminally differentiated odontoblasts and in the pulpal fibroblasts which can be divided by mitosis when required. Fibroblasts at various stages of differentiation co-exist in the human dental pulp. ATF-2 was investigated immunohistochemically in 20 permanent human teeth. According to the findings obtained, the mean percentage of ATF-2 positive cells was 68.5 ± 19.2 % in the odontoblasts and 22.8 ± 13.7 % in the pulpal fibroblasts. The comparison of ATF-2 positivity revealed a statistically significant difference between odontoblasts and pulpal fibroblasts. These findings have suggested that ATF-2 is more associated with cell survival rather than cell proliferation, and revealed much of effectiveness in maintaining terminal differentiation than the various differentiation stages of the cells.

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Acknowledgments

The authors wish to thank Dr. Humeyra Kocaelli (Istanbul University Faculty of Dentistry Department of Oral Surgery and Medicine, Istanbul, Turkey) for her valuable contribution in providing the necessary materials for this research, and Biologist Ilker Bolat (Istanbul University Faculty of Dentistry Department of Histology and Embryology, Istanbul, Turkey) for his assistance during the experimental work.

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  1. Division of Basic Sciences, Department of Histology and Embryology, Faculty of Dentistry, Istanbul University, Capa, Istanbul, Turkey

    Nurullah Keklikoglu & Sevtap Akinci

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  1. Nurullah Keklikoglu
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  2. Sevtap Akinci
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Correspondence to Nurullah Keklikoglu.

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Keklikoglu, N., Akinci, S. ATF-2 immunoreactivity in post-mitotic and terminally differentiated human odontoblasts. Med Mol Morphol 48, 164–168 (2015). https://doi.org/10.1007/s00795-014-0092-x

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  • DOI: https://doi.org/10.1007/s00795-014-0092-x

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