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Triglyceride metabolism in bone tissue is associated with osteoblast and osteoclast differentiation: a gene expression study

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Abstract

The role of bone marrow adipocytes in bone tissue is not yet understood. Adipocytes express enzymes for metabolism of free fatty acids and adipokines such as adiponectin, which have been shown to exert different effects on bone cells. Our aim was to find out whether triglyceride (TG) metabolism in bone tissue is associated with osteoblast and osteoclast differentiation by gene expression analysis of lipoprotein lipase (LPL), hormone sensitive lipase (HSL), fatty acid synthase (FASN), adiponectin, RUNX2, RANK, RANKL and OPG. Bone tissue was obtained from patients undergoing hip arthroplasty due to osteoporosis (OP) (50) or osteoarthritis (OA) (48) or from healthy autopsy controls (14). Lower bone mineral density and microstructural parameters were observed in OP compared to OA. The FASN expression did not differ between groups suggesting similar de novo lipogenesis. Lower LPL and HSL in OP suggest lower FFA release and uptake in OP bone tissue. Adiponectin expression was lower in OP than in OA and a trend was seen for controls. These results suggest OP bone has lower TG metabolism than OA and normal bone. In OP bone, lower osteoblastogenesis and higher osteoclast formation were observed and correlation analysis suggests adiponectin, LPL and HSL are associated with higher osteoblastogenesis and lower osteoclastogenesis. This study gives insights into TG metabolism in the human bone microenvironment. We conclude that OP bone tissue exhibits lower osteoblastogenesis, higher osteoclastogenesis and lower TG metabolism compared to OA or healthy controls.

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Acknowledgments

The study was supported by research programs P3-0298 and J3-2330 of the Research Agency of Slovenia. We thank the patients participating in our study for donating bone tissue. We thank Tina Žuran and Metka Košir for their help with RNA isolation and qPCR experiments, Viktor Jović for bone biopsies of autopsy cases and Franci Vindišar for OP and OA bone tissue.

Conflict of interest

All authors have no conflicts of interest.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, 1000, Ljubljana, Slovenia

    Jana Dragojevič, Janja Zupan & Janja Marc

  2. Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Korytkova ulica 2, 1000, Ljubljana, Slovenia

    Gregor Haring

  3. Clinical Department of Traumatology, University Medical Center, Zaloška cesta 7, 1525, Ljubljana, Slovenia

    Simon Herman

  4. Department of Traumatology, General and Teaching Hospital Celje, Oblakova ulica 5, 3000, Celje, Slovenia

    Radko Komadina

Authors
  1. Jana Dragojevič
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  2. Janja Zupan
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  3. Gregor Haring
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  4. Simon Herman
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  5. Radko Komadina
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  6. Janja Marc
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Corresponding author

Correspondence to Janja Marc.

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Dragojevič, J., Zupan, J., Haring, G. et al. Triglyceride metabolism in bone tissue is associated with osteoblast and osteoclast differentiation: a gene expression study. J Bone Miner Metab 31, 512–519 (2013). https://doi.org/10.1007/s00774-013-0445-x

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  • DOI: https://doi.org/10.1007/s00774-013-0445-x

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