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Simultaneous quantification of vasicine and vasicinone in different parts of Justicia adhatoda using high-performance thin-layer chromatography‒densitometry: comparison of different extraction techniques and solvent systems

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Abstract

The present study was carried out for screening an efficient extraction method and extracting solvent in different plant parts of Justicia adhatoda L. for the development of a rapid and simultaneous determination of vasicine and vasicinone using a validated high-performance thin-layer chromatography (HPTLC) method. Four different extraction techniques, viz., maceration extraction (ME), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE), and heat reflux extraction (HRE), as well as extracting solvents (water, methanol, acetone, and acetonitrile) were used. Chromatographic separation was carried out on pre-coated silica gel plate G60 F254 using ethyl acetate‒methanol‒ammonia (8:2:0.2, V/V) as the mobile phase, and densitometric analysis was carried out in absorbance mode at 270 nm for vasicine and vasicinone, respectively. The optimized mobile phase gave well-defined peaks at RF of 0.37 and 0.57 for vasicine and vasicinone, respectively. The calibration curve area versus concentration was found to be linear with regression coefficient (r) values of 0.9956 and 0.9919 in the range of 100‒400 ng band‒1 for vasicine and vasicinone, respectively. The HPTLC method was validated in terms of precision, specificity, recovery, and accuracy. The best quantitative estimation result for vasicine and vasicinone was obtained by microwave-assisted extraction in leaf parts using methanol as solvent. Under these conditions, it was found that this plant contained approximately 6.38 mg g‒1 vasicine and 3.59 mg g‒1 vasicinone, respectively. The present HPTLC method was found to be precise, reliable, and can be used for the quality control of J. adhatoda L. samples and different J. adhatoda extracted samples.

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References

  1. Roy DC, Shark M, Faruquee HM et al (2013) A Brief Review on Phytochemistry and Pharmacological Properties of Adhatoda vasica. J Trop Med Plants 14:115–124

    Google Scholar 

  2. Singh B, Sharma RA (2013) Anti-inflammatory and antimicrobial properties of pyrroloquinazoline alkaloids from Adhatoda vasica Nees. Phytomedicine 20:441–445. https://doi.org/10.1016/j.phymed.2012.12.015

    Article  CAS  PubMed  Google Scholar 

  3. Das C, Poi R, Chowdhury A (2005) A HPTLC determination of vasicine and vasicinone in Adhatoda vasica. Phytochem Anal Int J Plant Chem Biochem Tech 16(2):90–92. https://doi.org/10.1002/pca.817

    Article  CAS  Google Scholar 

  4. Claeson UP, Malmfors T, Wikman G, Bruhn JG (2000) Adhatoda vasica: a critical review of ethno pharmacological and toxicological data. J Ethnopharmacol 72:1–20. https://doi.org/10.1016/S0378-8741(00)00225-7

    Article  CAS  PubMed  Google Scholar 

  5. Lone SA, Yadav AS, Sharma AK, Tafazul M, Badkhane Y, Raghuwanshi DK (2013) A review on Adhatoda vasica Nees—an important and high demanded medicinal plant. Indo Am J Pharm 3:2600

    Google Scholar 

  6. Sudevan S, Parasivam R, Sundar S, Velauthan H, Ramasamy V (2019) Investigation of anti-inflammatory and anti-cancer activity of Justicia adathoda metabolites. Pak J Pharm Sci 32:1555–1561

    CAS  PubMed  Google Scholar 

  7. Brain KR, Thapa BB (1983) High-performance liquid chromatographic determination of vasicine and vasicinone in Adhatoda vasica Nees. J Chromatogr A 258:183–188. https://doi.org/10.1016/S0021-9673(00)96410-0

    Article  CAS  Google Scholar 

  8. Srivastava S, Verma RK, Gupta MM, Singh SC, Kumar S (2001) HPLC determination of vasicine and vasicinone in Adhatoda vasica with photo diode array detection. J Liq Chromatogr Relat 24:153–159. https://doi.org/10.1081/JLC-100001478

    Article  CAS  Google Scholar 

  9. Suthar AC, Katkar KV, Patil PS, Hamarapurkar PD, Mridula G, Naik VR, Mundada GR, Chauhan VS (2009) Quantitative estimation of vasicine and vasicinone in Adhatoda vasica by HPTLC. J Pharm Res 2:1893–1899

    CAS  Google Scholar 

  10. Murali RS, Rao GN, Basavaraju R (2016) Liquid chromatography coupled to mass spectrometry based identification of elite chemotypes of Adhatoda vasica Nees for profitable agronomy—a farmer centric approach. Indian J Sci Technol 9:26. https://doi.org/10.1002/jssc.201900509

    Article  CAS  Google Scholar 

  11. Jamal M, Amir M, Ali Z, Mujeeb M (2018) A comparative study for the extraction methods and solvent selection for isolation, quantitative estimation and validation of ursolic acid in the leaves of Lantana camara by HPTLC method. Future J Pharm Sci 4:229–233. https://doi.org/10.1016/j.fjps.2018.07.002

    Article  Google Scholar 

  12. Dirar AI, Alsaadi DHM, Wada M, Mohamed MA, Watanabe T, Devkota HP (2019) Effects of extraction solvents on total phenolic and flavonoid contents and biological activities of extracts from Sudanese medicinal plants. S Afr J Bot 120:261–267. https://doi.org/10.1016/j.sajb.2018.07.003

    Article  CAS  Google Scholar 

  13. Stalikas CD (2007) Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci 30:3268–3295. https://doi.org/10.1002/jssc.200700261

    Article  CAS  PubMed  Google Scholar 

  14. Kaur P, Gupta RC, Dey A, Pandey DK (2019) Simultaneous quantification of oleanolic acid, ursolic acid, betulinic acid and lupeol in different populations of five Swertia species by using HPTLC–densitometry: comparison of different extraction methods and solvent selection. Ind Crops Prod 130:537–546. https://doi.org/10.1016/j.indcrop.2018.12.089

    Article  CAS  Google Scholar 

  15. Wang L, Weller CL (2006) Recent advances in extraction of nutraceuticals from plants. Trends Food Sci Technol 17:300–312. https://doi.org/10.1016/j.tifs.2005.12.004

    Article  CAS  Google Scholar 

  16. Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH (2014) Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal 22:296–302. https://doi.org/10.1016/j.jfda.2013.11.001

    Article  CAS  PubMed  Google Scholar 

  17. Truong DH, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC (2019) Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. J Food Qual 2019:8178294. https://doi.org/10.1155/2019/8178294

    Article  CAS  Google Scholar 

  18. Ray A, Dash B, Sahoo S, Sahoo A, Jena S, Kar B, Chatterjee T, Ghosh B, Nayak S (2017) Development and validation of an HPTLC method for estimation of coronarin D in Hedychium coronarium rhizome. Acta Chromatogr 29(3):415–426. https://doi.org/10.1556/1326.2017.29.3.11

    Article  CAS  Google Scholar 

  19. Sultana B, Anwar F, Ashraf M (2009) Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 14(6):2167–2180

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to Prof. (Dr.) S.C. Si, Dean, Center of Biotechnology and Prof. (Dr.) M.R. Nayak, President, Siksha ‘O’ Anusandhan University, for providing financial support and encouragement.

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Authors and Affiliations

  1. Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Bhubaneswar, 751003, India

    Bhuban Mohan Padhiari, Asit Ray, Sudipta Jena, Bibhuti Bhusan Champati, Ambika Sahoo, Swagat Mohanty & Sanghamitra Nayak

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  1. Bhuban Mohan Padhiari
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  2. Asit Ray
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Correspondence to Sanghamitra Nayak.

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Padhiari, B.M., Ray, A., Jena, S. et al. Simultaneous quantification of vasicine and vasicinone in different parts of Justicia adhatoda using high-performance thin-layer chromatography‒densitometry: comparison of different extraction techniques and solvent systems. JPC-J Planar Chromat 33, 599–607 (2020). https://doi.org/10.1007/s00764-020-00070-9

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  • DOI: https://doi.org/10.1007/s00764-020-00070-9

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