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Genetic diversity of strawberry mild yellow edge virus from eastern Canada

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Abstract

Strawberry mild yellow edge virus (SMYEV) is a member of the genus Potexvirus, family Alphaflexiviridae. It is one of the most common pathogenic viruses infecting cultivated strawberries worldwide. In this study, we investigated the genetic diversity of SMYEV in strawberry fields that were severely affected by strawberry decline disease in the eastern Canadian provinces of New Brunswick, Nova Scotia, Prince Edward Island and Quebec. A total of 134 SMYEV coat protein (CP) gene sequences, representing 85 nucleic acid haplotypes, were identified in 56 field samples. A highly divergent SMYEV population was found in all four provinces, but there was little genetic differentiation among the populations, and moreover, the Canadian SMYEV isolates formed a unique dissimilar, genetically divergent population group when compared to those reported in other countries. Phylogenetic analysis revealed three new SMYEV subclades that consisted mainly of Canadian variants and were composed of 76 sequence haplotypes (76/85, 88%). Mixed infections by different SMYEV variants were observed in 38 samples (38/56, 68%). Evolutionary analysis suggested that the SMYEV strains in eastern Canada possibly originated outside of Canada but adapted to conditions in the region through genetic mutations.

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Acknolwedgements

We thank Pervaiz Abbasi, Richard Hogue, Chris Jordan, John Lewis, and Mathuresh Singh for collecting samples from strawberry fields. This study was funded by Agriculture and Agri-Food Canada (Project J-000051 and Project J-001564).

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Authors and Affiliations

  1. Agriculture and Agri-Food Canada, Summerland Research and Development Centre, Summerland, BC, V0H 1Z0, Canada

    Yu Xiang, Mike Bernardy, Li Su, Basdeo Bhagwat, Janesse Holmes & Jenna M. Grose

  2. Agriculture and Agri-Food Canada, Fredericton Research and Development Centre, Fredericton, NB, E3B 4Z7, Canada

    Xianzhou Nie, Virginia Dickison & Alexa C. Creelman

  3. Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, Victoria, BC, V8Z 1M5, Canada

    Jun-jun Liu

  4. College of Horticulture, Northwest Agriculture and Forest University, Yangling, Shaanxi, 712100, China

    Li Su

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  1. Yu Xiang
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  2. Xianzhou Nie
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  3. Mike Bernardy
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  4. Jun-jun Liu
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  5. Li Su
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  6. Basdeo Bhagwat
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  7. Virginia Dickison
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  8. Janesse Holmes
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  9. Jenna M. Grose
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  10. Alexa C. Creelman
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Correspondence to Yu Xiang or Xianzhou Nie.

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Xiang, Y., Nie, X., Bernardy, M. et al. Genetic diversity of strawberry mild yellow edge virus from eastern Canada. Arch Virol 165, 923–935 (2020). https://doi.org/10.1007/s00705-020-04561-2

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