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Development of an ELISA for serological detection of feline morbillivirus infection

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Abstract

Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that was discovered in 2012. Despite the importance of FeMV infection in cats because of its postulated involvement in kidney diseases, no simple serological assay has been reported in its detection. Here, FeMV phosphoprotein (P protein) was expressed and purified as a glutathione-S-transferase (GST)-fusion protein and used for an enzyme-linked immunosorbent assay (ELISA) to detect FeMV-specific antibodies. With a cutoff value determined by immunoblotting, anti-FeMV P protein was detected with this assay in 22 (22%) of the 100 cat plasma samples collected from various regions of Japan. This ELISA is useful for epidemiological and immunological studies, as well as for diagnosis of FeMV infection.

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Acknowledgements

This work was supported by JSPS KAKENHI Grant Number 15K07719.

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Authors and Affiliations

  1. Laboratory of Veterinary Microbiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan

    Kaho Arikawa, Akiko Wachi, Yuri Imura, Kripitch Sutummaporn & Tetsuya Furuya

  2. Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan

    Chieko Kai

  3. Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan

    Eun-Sil Park & Shigeru Morikawa

  4. Canine-Lab. Inc., Tokyo, 184-0003, Japan

    Yosuke Uematsu & Tomohiro Yamaguchi

Authors
  1. Kaho Arikawa
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  2. Akiko Wachi
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  3. Yuri Imura
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  4. Kripitch Sutummaporn
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  5. Chieko Kai
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  6. Eun-Sil Park
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  7. Shigeru Morikawa
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  8. Yosuke Uematsu
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  9. Tomohiro Yamaguchi
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  10. Tetsuya Furuya
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Corresponding author

Correspondence to Tetsuya Furuya.

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All the authors declare that they have no conflict of interest.

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This article does not contain any studies with animals performed by any of the authors.

Funding

This study was funded by JSPS KAKENHI (grant number 15K07719).

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Arikawa, K., Wachi, A., Imura, Y. et al. Development of an ELISA for serological detection of feline morbillivirus infection. Arch Virol 162, 2421–2425 (2017). https://doi.org/10.1007/s00705-017-3386-1

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  • DOI: https://doi.org/10.1007/s00705-017-3386-1

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