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Absence of vaccinia virus detection in a remote region of the Northern Amazon forests, 2005-2015

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Abstract

Vaccinia virus (VACV) circulates in Brazil and other South America countries and is responsible for a zoonotic disease that usually affects dairy cattle and humans, causing economic losses and impacting animal and human health. Furthermore, it has been detected in wild areas in the Brazilian Amazon. To better understand the natural history of VACV, we investigated its circulation in wildlife from French Guiana, a remote region in the Northern Amazon forest. ELISA and plaque reduction neutralization tests were performed to detect anti-orthopoxvirus antibodies. Real-time and standard PCR targeting C11R, A56R and A26L were applied to detect VACV DNA in serum, saliva and tissue samples. No evidence of VACV infection was found in any of the samples tested. These findings provide additional information on the VACV epidemiological puzzle. The virus could nevertheless be circulating at low levels that were not detected in areas where no humans or cattle are present.

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Acknowledgements

We thank colleagues from the Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane for their excellent technical support. We also thank Pró-Reitoria de Pesquisa from Universidade Federal de Minas Gerais (PRPq/UFMG), CAPES, FAPEMIG and CNPq. JS Abrahão, EG Kroon, and G de Souza Trindade are researchers from CNPq.

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Authors and Affiliations

  1. Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil

    Galileu Barbosa Costa, Betânia Paiva Drumond, Jônatas Santos Abrahão, Erna Geessien Kroon & Giliane de Souza Trindade

  2. Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, 23 Avenue Pasteur, BP 6010, 97306, Cayenne, French Guiana

    Anne Lavergne, Edith Darcissac, Vincent Lacoste & Benoît de Thoisy

Authors
  1. Galileu Barbosa Costa
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  2. Anne Lavergne
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  3. Edith Darcissac
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  4. Vincent Lacoste
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  5. Betânia Paiva Drumond
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  6. Jônatas Santos Abrahão
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  7. Erna Geessien Kroon
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  8. Benoît de Thoisy
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  9. Giliane de Souza Trindade
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Corresponding authors

Correspondence to Galileu Barbosa Costa or Giliane de Souza Trindade.

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Funding

This study was funded by two European projects, CAROLIA and RESERVOIRS, through ERDF funds and a European Commission “REGPOT-CT-2011-285837-STRonGer” grant within the FP7. This work also benefited from an “Investissements d’Avenir’ grant managed by Agence Nationale de la Recherche, France (CEBA, ref. ANR-10-LABX-25-01).

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

Capture of rodents, bats and marsupials does not require an ethics committee in French Guiana.

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Costa, G.B., Lavergne, A., Darcissac, E. et al. Absence of vaccinia virus detection in a remote region of the Northern Amazon forests, 2005-2015. Arch Virol 162, 2369–2373 (2017). https://doi.org/10.1007/s00705-017-3353-x

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  • DOI: https://doi.org/10.1007/s00705-017-3353-x

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