Skip to main content
Log in

Complete genome sequence of yacon necrotic mottle virus, a novel putative member of the genus Badnavirus

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

The complete genome sequence of a previously undescribed virus isolated from a yacon plant exhibiting necrotic mottle, chlorosis, stunting, and leaf malformation symptoms in Gyeongju, Korea, was determined. The genome of this virus consists of one circular double-stranded DNA of 7661 bp in size. The genome contained four open reading frames (ORFs 1 to 4) on the plus strand that potentially encode proteins of 26, 32, 234, and 25 kDa. Protein BLAST analysis showed that ORF3, which is the largest ORF, has 45 % amino acid sequence identity (with 89 % coverage) to the ORF3 of fig badnavirus 1 (FBV-1), a recently identified badnavirus. Phylogenetic analysis provided further evidence that the virus identified in this study is probably a member of a new species in the genus Badnavirus. The name yacon necrotic mottle virus (YNMoV) is proposed for this new virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Gayral P, Noa-Carrazana JC, Lescot M, Lheureux F, Lockhart BE, Matsumoto T, Piffanelli P, Iskra-Caruana ML (2008) A single Banana streak virus integration event in the banana genome as the origin of infectious endogenous pararetrovirus. J Virol 82:6697–6710

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Geering AD, Olszewski NE, Harper G, Lockhart BE, Hull R, Thomas JE (2005) Banana contains a diverse array of endogenous badnaviruses. J Gen Virol 86:511–520

    Article  CAS  PubMed  Google Scholar 

  3. Geering AD, Parry JN, Thomas JE (2011) Complete genome sequence of a novel badnavirus, banana streak IM virus. Arch Virol 156:733–737

    Article  CAS  PubMed  Google Scholar 

  4. Geering ADW, Hull R (2012) Family Caulimoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier, London

    Google Scholar 

  5. Harper G, Osuji JO, Heslop-Harrison JS, Hull R (1999) Integration of banana streak badnavirus into the Musa genome: molecular and cytogenetic evidence. Virology 255:207–213

    Article  CAS  PubMed  Google Scholar 

  6. Harper G, Hart D, Moult S, Hull R (2004) Banana streak virus is very diverse in Uganda. Virus Res 100:51–56

    Article  CAS  PubMed  Google Scholar 

  7. Iskra-Caruana ML, Duroy PO, Chabannes M, Muller E (2014) The common evolutionary history of badnaviruses and banana. Infect Genet Evol 21:83–89

    Article  CAS  PubMed  Google Scholar 

  8. Laney AG, Hassan M, Tzanetakis IE (2012) An integrated badnavirus is prevalent in fig germplasm. Phytopathology 102:1182–1189

    Article  CAS  PubMed  Google Scholar 

  9. Schelhorn SE, Fischer M, Tolosi L, Altmuller J, Nurnberg P, Pfister H, Lengauer T, Berthold F (2013) Sensitive detection of viral transcripts in human tumor transcriptomes. PLoS Comput Biol 9:e1003228

    Article  PubMed Central  PubMed  Google Scholar 

  10. Seal S, Turaki A, Muller E, Kumar PL, Kenyon L, Filloux D, Galzi S, Lopez-Montes A, Iskra-Caruana ML (2014) The prevalence of badnaviruses in West African yams (Dioscorea cayenensis-rotundata) and evidence of endogenous pararetrovirus sequences in their genomes. Virus Res 186:144–154

    Article  CAS  PubMed  Google Scholar 

  11. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Valentova K, Cvak L, Muck A, Ulrichova J, Simanek V (2003) Antioxidant activity of extracts from the leaves of Smallanthus sonchifolius. Eur J Nutr 42:61–66

    Article  PubMed  Google Scholar 

  13. Xu D, Mock R, Kinard G, Li R (2011) Molecular analysis of the complete genomic sequences of four isolates of Gooseberry vein banding associated virus. Virus Genes 43:130–137

    Article  CAS  PubMed  Google Scholar 

  14. Yan X, Suzuki M, Ohnishi-Kameyama M, Sada Y, Nakanishi T, Nagata T (1999) Extraction and identification of antioxidants in the roots of yacon (Smallanthus sonchifolius). J Agric Food Chem 47:4711–4713

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research was supported by a grant from the Agenda Program (PJ008841), funded by the Rural Development Administration of Korea.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jang-Kyun Seo.

Additional information

Y.-J. Lee and H. R. Kwak contributed equally to this work.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 11 kb)

Supplementary material 2 (TXT 15 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lee, YJ., Kwak, HR., Lee, YK. et al. Complete genome sequence of yacon necrotic mottle virus, a novel putative member of the genus Badnavirus . Arch Virol 160, 1139–1142 (2015). https://doi.org/10.1007/s00705-015-2341-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-015-2341-2

Keywords

Navigation