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Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation

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Abstract

Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, has a single-stranded DNA genome. TYLCV can induce severe disease symptoms on tomato plants, but other hosts plants such as cucurbits and peppers are asymptomatic. A full-length DNA clone of a Korean TYLCV isolate was constructed by rolling-circle amplification from TYLCV-infected tomatoes in Korea. To assess relative susceptibility of sweet pepper varieties to TYLCV, 19 cultivars were inoculated with cloned TYLCV by agro-inoculation. All TYLCV-infected sweet peppers were asymptomatic, even though Southern hybridization and polymerase chain reaction analysis showed TYLCV genomic DNA accumulation in roots, stems, and newly produced shoots. Southern hybridization indicated that TYLCV replicated and moved systemically from agro-inoculated apical shoot tips to roots or newly produced shoots of sweet peppers. Whitefly-mediated inoculation experiments showed that TYLCV can be transmitted to tomatoes from TYLCV-infected sweet peppers. Taken together, these results indicate that sweet pepper can be a reservoir for TYLCV in nature.

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Acknowledgment

This research was supported by a grant from the iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries: No. 311058-05-1-HD140), Ministry for Agriculture, Food, and Rural Affairs, Republic of Korea.

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Authors and Affiliations

  1. Department of Genetic Engineering, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon, 440-746, Republic of Korea

    Eui-Joon Kil, Hee-Seong Byun, Sunhoo Kim, Jaedeok Kim, Jungan Park, Seungchan Cho & Sukchan Lee

  2. Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Suwon, 440-400, Republic of Korea

    Jungan Park

  3. Breeding Institute, Nongwoo Bio. Co., Ltd, Yeoju, 469-885, Republic of Korea

    Dong-Cheol Yang

  4. Institute of Plant Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Kyeong-Yeoll Lee

  5. Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Republic of Korea

    Hong-Soo Choi

  6. Future High-Technology Agriculture Research Division, Research and Development Bureau, Chungcheongnam-do Agricultural Research and Extension Services, 365 Jonggyeong-ri, Yesan, 340-861, Republic of Korea

    Ji-Kwang Kim

Authors
  1. Eui-Joon Kil
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  2. Hee-Seong Byun
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  8. Kyeong-Yeoll Lee
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Corresponding authors

Correspondence to Ji-Kwang Kim or Sukchan Lee.

Additional information

E.-J. Kil and H.-S. Byun contributed equally to this work.

Electronic supplementary material

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705_2014_2072_MOESM1_ESM.pptx

Phylogenetic tree based on the full-length genome sequence of TYLCV isolates. This tree was generated by the neighbor-joining method using MEGA5 software. Tree branches were bootstrapped with 2000 replications (PPTX 240 kb)

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Kil, EJ., Byun, HS., Kim, S. et al. Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation. Arch Virol 159, 2387–2395 (2014). https://doi.org/10.1007/s00705-014-2072-9

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  • DOI: https://doi.org/10.1007/s00705-014-2072-9

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