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Complete nucleotide sequence and construction of an infectious clone of Chinese yam necrotic mosaic virus suggest that macluraviruses have the smallest genome among members of the family Potyviridae

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Abstract

The complete nucleotide sequence of Chinese yam necrotic mosaic virus (CYNMV) was determined from cloned virus cDNA. The CYNMV genomic RNA is 8224 nucleotides in length, excluding the poly(A) tail, and contains one long open reading frame encoding a large polyprotein of 2620 amino acids. CYNMV has no counterpart to the P1 cistron and a short HC-Pro cistron located at the 5′ side of the potyvirus genome. A full-length cDNA clone, pCYNMV, was assembled under the control of the cauliflower mosaic virus 35S promoter and the nopaline synthase terminator. Biolistic inoculation of Nagaimo plants with cDNA resulted in systemic necrotic mosaic symptoms typical of CYNMV infection. To our knowledge, this is the first report of the complete nucleotide sequence and construction of an infectious cDNA clone of a member of the genus Macluravirus.

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References

  1. Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471–487

    Article  PubMed  CAS  Google Scholar 

  2. Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479

    Article  PubMed  CAS  Google Scholar 

  3. Adams MJ, Zerbini FM, French R, Rabenstein F, Stenger DC, Valkonen JPT (2012) Family Potyviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Academic Press, London, pp 1069–1089

    Google Scholar 

  4. Badge J, Robinson DJ, Brunt AA, Foster GD (1997) 3′-Terminal sequences of the RNA genomes of narcissus latent and Maclura mosaic viruses suggest that they represent a new genus of the Potyviridae. J Gen Virol 78:253–257

    PubMed  CAS  Google Scholar 

  5. Bowen AJ, Clarke IT, Foster GD (2003) Review of the Genus Macluravirus of the Potyviridae. Bulg J Agric Sci 9:277–286

    Google Scholar 

  6. Chung BY-W, Miller WA, Atkins JF, Firth AE (2008) An overlapping essential gene in the Potyviridae. Proc Natl Acad Sci USA 105:5897–5902

    Article  PubMed  CAS  Google Scholar 

  7. Ciuffo M, Testa M, Lenzi R, Turina M (2011) Ranunculus latent virus: a strain of artichoke latent virus or a new macluravirus infecting artichoke? Arch Virol 156:1053–1057

    Article  PubMed  CAS  Google Scholar 

  8. Desbiez C, Girard M, Lecoq H (2010) A novel natural mutation in HC-Pro responsible for mild symptomatology of Zucchini yellow mosaic virus (ZYMV, Potyvirus) in cucurbits. Arch Virol 155:397–401

    Article  PubMed  CAS  Google Scholar 

  9. French R, Stenger DC (2005) Genome sequences of Agropyron mosaic virus and Hordeum mosaic virus support reciprocal monophyly of the genera Potyvirus and Rymovirus in the family Potyviridae. Arch Virol 150:299–312

    Article  PubMed  CAS  Google Scholar 

  10. Fukumoto F, Tochihara H (1978) Chinese yam necrotic mosaic virus (in Japanese with English abstract). Ann Phytopathol Soc Jpn 44:1–5

    Article  Google Scholar 

  11. Gal-On A (2000) A point mutation in the FRNK motif of the Potyvirus helper component-protease gene alters symptom expression in cucurbits and elicits protection against the severe homologous virus. Phytopathology 90:467–473

    Article  PubMed  CAS  Google Scholar 

  12. Gal-On A, Meiri E, Huet H, Hua WJ, Raccah B, Gaba V (1995) Particle bombardment drastically increases the infectivity of cloned DNA of zucchini yellow mosaic potyvirus. J Gen Virol 76:3223–3227

    Article  PubMed  CAS  Google Scholar 

  13. Gibbs AJ, Ohshima K, Phillips MJ, Gibbs MJ (2008) The prehistory of potyviruses: their initial radiation was during the dawn of agriculture. PLoS One 3:e2523. doi:10.1371/journal.pone.0002523

    Article  PubMed  Google Scholar 

  14. International Committee on Taxonomy of Viruses (2011) Virus Taxonomy: 2011 Release. http://www.ictvonline.org/virusTaxonomy.asp?version=2011. Accessed 22 Mar 2012

  15. Jebasingh T, Jose M, Yadunandam AK, Backiyarani S, Srividhya KV, Krishnaswamy S, Usha R (2011) Molecular modeling and conformational analysis of native and refolded viral genome-linked protein of Cardamom mosaic virus. Indian J Biochem Biophys 48:336–340

    PubMed  CAS  Google Scholar 

  16. Kadaré G, Haenni A-L (1997) Virus-encoded RNA helicases. J Virol 71:2583–2590

    PubMed  Google Scholar 

  17. Kamer G, Argos P (1984) Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Res 12:7269–7282

    Article  PubMed  CAS  Google Scholar 

  18. Kashiwazaki S, Hayano Y, Minobe Y, Omura T, Hibino H, Tsuchizaki T (1989) Nucleotide sequence of the capsid protein gene of barley yellow mosaic virus. J Gen Virol 70:3015–3023

    Article  PubMed  CAS  Google Scholar 

  19. Kasschau KD, Carrington JC (2001) Long-distance movement and replication maintenance functions correlate with silencing suppression activity of potyviral HC-Pro. Virology 285:71–81

    Article  PubMed  CAS  Google Scholar 

  20. Kondo T (2001) The 3′-terminal sequence of Chinese yam necrotic mosaic virus genomic RNA: a close relationship with macluravirus. Arch Virol 146:1527–1535

    Article  PubMed  CAS  Google Scholar 

  21. Kondo T, Fuji S, Yamashita K, Kang D-K, Chang M-U (2005) Broad bean wilt virus 2 in yams. J Gen Plant Pathol 71:441–443

    Article  Google Scholar 

  22. Kondo T, Kang D-K, Fuji S, Chang M-U (2003) Sequence diversity in the coat protein and 3′-untranslated region of Chinese yam necrotic mosaic virus RNA. J Gen Plant Pathol 69:397–399

    Article  CAS  Google Scholar 

  23. Kondo T, Kasai K, Yamashita K, Ishitani M (2007) Selection and discrimination of an attenuated strain of Chinese yam necrotic mosaic virus for cross-protection. J Gen Plant Pathol 73:152–155

    Article  CAS  Google Scholar 

  24. Li W, Hilf ME, Webb SE, Baker CA, Adkins S (2008) Presence of P1b and absence of HC-Pro in squash vein yellowing virus suggests a general feature of the genus Ipomovirus in the family Potyviridae. Virus Res 135:213–219

    Article  PubMed  CAS  Google Scholar 

  25. Liou RF, Yan HZ, Hong JL (2003) Molecular evidence that aphid-transmitted Alpinia mosaic virus is a tentative member of the genus Macluravirus. Arch Virol 148:1211–1218

    Article  PubMed  CAS  Google Scholar 

  26. López-Moya JJ, Wang RY, Pirone TP (1999) Context of the coat protein DAG motif affects potyvirus transmissibility by aphids. J Gen Virol 80:3281–3288

    PubMed  Google Scholar 

  27. Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA (1987) Selection of AUG initiation codons differs in plants and animals. EMBO J 6:43–48

    PubMed  Google Scholar 

  28. Mandal B, Vijayanandraj S, Shilpi S, Pun KB, Singh V, Pant RP, Jain RK, Varadarasan S, Varma A (2012) Disease distribution and characterization of a new macluravirus associated with chirke disease of large cardamom. Ann Appl Biol. doi:10.1111/j.1744-7348.2012.00537.x

    Google Scholar 

  29. Nishiguchi M, Kobayashi K (2011) Attenuated plant viruses: preventing virus diseases and understanding the molecular mechanism. J Gen Plant Pathol 77:221–229

    Article  Google Scholar 

  30. Plisson C, Drucker M, Blanc S, German-Retana S, Gall OL, Thomas D, Bron P (2003) Structural characterization of HC-Pro, a plant virus multifunctional protein. J Biol Chem 278:23753–23761

    Article  PubMed  CAS  Google Scholar 

  31. Rohožková J, Navrátil M (2011) P1 peptidase: a mysterious protein of family Potyviridae. J Biosci 36:189–200

    Article  PubMed  Google Scholar 

  32. Ryan MD, Flint M (1997) Virus-encoded proteinases of the picornavirus super-group. J Gen Virol 78:699–723

    PubMed  CAS  Google Scholar 

  33. Sano T, Higaki T, Oda Y, Hayashi T, Hasezawa S (2005) Appearance of actin microfilament ‘twin peaks’ in mitosis and their function in cell plate formation, as visualized in tobacco BY-2 cells expressing GFP-fimbrin. Plant J 44:595–605

    Article  PubMed  CAS  Google Scholar 

  34. Shiboleth YM, Haronsky E, Leibman D, Arazi T, Wassenegger M, Whitham SA, Gaba V, Gal-On A (2007) The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development. J Virol 81:13135–13148

    Article  PubMed  CAS  Google Scholar 

  35. Shirako Y, Ehara Y (1986) Rapid diagnosis of Chinese yam necrotic mosaic virus infection by electro-blot immunoassay. Ann Phytopathol Soc Japan 52:453–459

    Article  Google Scholar 

  36. Shukla DD, Ward CW, Brunt AA (1994) The Potyviridae. CAB International, Wallingford

    Google Scholar 

  37. Stenger DC, French R (2004) Complete nucleotide sequence of Oat necrotic mottle virus: a distinct Tritimovirus species (family Potyviridae) most closely related to Wheat streak mosaic virus. Arch Virol 149:633–640

    Article  PubMed  CAS  Google Scholar 

  38. Suehiro N, Natsuaki T, Watanabe T, Okuda S (2004) An important determinant of the ability of Turnip mosaic virus to infect Brassica spp. and/or Raphanus sativus is in its P3 protein. J Gen Virol 85:2087–2098

    Article  PubMed  CAS  Google Scholar 

  39. Susaimuthu J, Tzanetakis IE, Gergerich RC, Martin RR (2008) A member of a new genus in the Potyviridae infects Rubus. Virus Res 131:145–151

    Article  PubMed  CAS  Google Scholar 

  40. Tatineni S, Ziems AD, Wegulo SN, French R (2009) Triticum mosaic virus: a distinct member of the family Potyviridae with an unusually long leader sequence. Phytopathology 99:943–950

    Article  PubMed  CAS  Google Scholar 

  41. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  42. Tochihara H (1993) Yams (in Japanese). In: Tsuchizaki T, Tochihara H, Kameya M, Yanase H (eds) Plant virus diseases in Japan. Zenkoku Noson Kyoiku Kyokai, Tokyo, pp 431–435

    Google Scholar 

  43. Urcuqui-Inchima S, Haenni A-L, Bernardi F (2001) Potyvirus proteins: a wealth of functions. Virus Res 74:157–175

    Article  PubMed  CAS  Google Scholar 

  44. Wang J-G, Zou X-J, Zheng H-Y, Adams MJ, Chen H-R, Chen JP (2009) Molecular characterization of a new macluravirus from yam in Yunnan, China. Arch Virol 154:1379–1380

    Article  PubMed  CAS  Google Scholar 

  45. Yon J, Fried M (1989) Precise gene fusion by PCR. Nucleic Acids Res 17:4895

    Article  PubMed  CAS  Google Scholar 

  46. You Y, Shirako Y (2010) Bymovirus reverse genetics: requirements for RNA2-encoded proteins in systemic infection. Mol Plant Pathol 11:383–394

    Article  PubMed  CAS  Google Scholar 

  47. Zheng T, Chen J, Chen JP, Adams MJ (2002) The complete sequence of Oat mosaic virus and evidence for deletion and duplication in RNA2. Arch Virol 147:635–642

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Dr. S. Hasezawa (Graduate School of Frontier Sciences, The University of Tokyo, Japan) and Dr. T. Sano (Faculty of Bioscience and Applied Chemistry, Hosei University, Japan) for providing the GFP vector, and Dr. N. Noda (National Institute of Floricultural Science, Japan) for helpful advice. This work was supported by the Research for Promoting Technological Seeds program of the Japan Science and Technology Agency.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Agriculture Research Institute, Aomori Prefectural Industrial Technology Research Center, 82-9 Tanaka, Kuroishi, Aomori, 036-0522, Japan

    Toru Kondo

  2. Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori, Japan

    Takashi Fujita

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  1. Toru Kondo
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  2. Takashi Fujita
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Correspondence to Toru Kondo.

Additional information

Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AB710145.

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Kondo, T., Fujita, T. Complete nucleotide sequence and construction of an infectious clone of Chinese yam necrotic mosaic virus suggest that macluraviruses have the smallest genome among members of the family Potyviridae . Arch Virol 157, 2299–2307 (2012). https://doi.org/10.1007/s00705-012-1429-1

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