Skip to main content

Advertisement

SpringerLink
Log in

Prevalence of hepatitis E virus and sapovirus in post-weaning pigs and identification of their genetic diversity

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Hepatitis E virus (HEV) and sapovirus (SaV) induce acute hepatitis and gastroenteritis, respectively, in humans. As pigs have been recognized as an important reservoir for these viruses, we evaluated the infection rates of both viruses using fecal samples collected from post-weaning pigs via RT-PCR methods. In the five swine farms assessed in this study, 3 farms were found to be HEV-positive and 4 farms were SaV-positive. The overall prevalence of HEV and SaV in the pigs was 17.0 and 23.1%, respectively. Phylogenetic tree analysis revealed that the isolated swine HEVs belonged to genotype 3 and the porcine SaVs belonged to genogroup III. This study proved that both HEV and SaV are prevailing in post-weaning pigs in Korea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Ahn JM, Kang SG, Lee DY, Shin SJ, Yoo HS (2005) Identification of novel human hepatitis E virus (HEV) isolates and determination of the seroprevalence of HEV in Korea. J Clin Microbiol 43:3042–3048

    Article  PubMed  CAS  Google Scholar 

  2. Arankalle VA, Joshi MV, Kulkarni AM, Ganhe SS, Chobe LP, Rautmare SS, Mishra AC, Padbidri VS (2001) Prevalence of anti-hepatitis E virus antibodies in different Indian animal species. J Viral Hepat 8:223–227

    Article  PubMed  CAS  Google Scholar 

  3. Choi IS, Kwon HJ, Shin NR, Yoo HS (2003) Identification of swine hepatitis E virus (HEV) and prevalence of anti-HEV antibodies in swine and human populations in Korea. J Clin Microbiol 41:3602–3608

    Article  PubMed  CAS  Google Scholar 

  4. Chiba S, Sakuma Y, Kogasaka R, Akihara M, Horino K, Nakao T, Fukui S (1979) An outbreak of gastroenteritis associated with calicivirus in an infant home. J Med Virol 4:249–254

    Article  PubMed  CAS  Google Scholar 

  5. Erker JC, Desai SM, Schlauder GG, Dawson GJ, Mushahwar IK (1999) A hepatitis E virus variant from the United States: molecular characterization and transmission in cynomolgus macaques. J Gen Virol 80:681–690

    PubMed  CAS  Google Scholar 

  6. Farkas T, Zhong WM, Jing Y, Huang PW, Espinosa SM, Martinez N, Morrow AL, Ruiz-Palacios GM, Pickering LK, Jing X (2004) Genetic diversity among sapoviruses. Arch Virol 149:1309–1323

    Article  PubMed  CAS  Google Scholar 

  7. Guo M, Chang KO, Hardy ME, Zhang Q, Parwani AV, Saif LJ (1999) Molecular characterization of a porcine enteric calicivirus genetically related to Sapporo-like human caliciviruses. J Virol 73:9625–9631

    PubMed  CAS  Google Scholar 

  8. Guo M, Hayes J, Cho KO, Parwani AV, Lucas LM, Saif LJ (2001) Comparative pathogenesis of tissue culture-adapted and wild-type Cowden porcine enteric calicivirus (PEC) in gnotobiotic pigs and induction of diarrhea by intravenous inoculation of wild-type PEC. J Virol 75:9239–9251

    Article  PubMed  CAS  Google Scholar 

  9. Hansman GS, Oka T, Katayama K, Takeda N (2007) Human sapoviruses: genetic diversity, recombination, and classification. Rev Med Virol 17:133–141

    Article  PubMed  CAS  Google Scholar 

  10. Jiang X, Huang PW, Zhong WM, Farkas T, Cubitt DW, Matson DO (1999) Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. J Virol Methods 83:145–154

    Article  PubMed  CAS  Google Scholar 

  11. Kasorndorkbua C, Guenette DK, Huang FF, Thomas PJ, Meng XJ, Halbur PG (2004) Routes of transmission of swine hepatitis E virus in pigs. J Clin Microbiol 42:5047–5052

    Article  PubMed  CAS  Google Scholar 

  12. Lu L, Li C, Hagedorn CH (2006) Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis. Rev Med Virol 16:5–36

    Article  PubMed  CAS  Google Scholar 

  13. Meng XJ, Purcell RH, Halbur PG, Lehman JR, Webb DM, Tsareva TS, Haynes JS, Thacker BJ, Emerson SU (1997) A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci USA 94:9860–9865

    Article  PubMed  CAS  Google Scholar 

  14. Mizuo H, Yazaki Y, Sugawara K, Tsuda F, Takahashi M, Nishizawa T, Okamoto H (2005) Possible risk factors for the transmission of hepatitis E virus and for the severe form of hepatitis E acquired locally in Hokkaido, Japan. J Med Virol 76:341–349

    Article  PubMed  CAS  Google Scholar 

  15. Nishizawa T, Takahashi M, Mizuo H, Miyajima H, Gotanda Y, Okamoto H (2003) Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99% identity over the entire genome. J Gen Virol 84:1245–1251

    Article  PubMed  CAS  Google Scholar 

  16. Okamoto H, Takahashi M, Nishzawa T, Fukai K, Muramatsu U, Yoshikawa A (2001) Analysis of the complete genome of indigenous swine hepatitis E virus isolated in Japan. Biochem Biophys Res Commun 289:929–936

    Article  PubMed  CAS  Google Scholar 

  17. Panda SK, Thakral D, Rehman S (2007) Hepatitis E virus. Rev Med Virol 17:151–180

    Article  PubMed  CAS  Google Scholar 

  18. Saif LJ, Bohl EH, Theil KW, Cross RF, House JA (1980) Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. J Clin Microbiol 12:105–111

    PubMed  CAS  Google Scholar 

  19. Schlauder GG, Dawson Gj, Erker JC, Kwo PY, Knigge MF, Smalley DL, Rosenblatt JE, Desai SM, Mushahwar IK (1998) The sequence and phylogentic analysis of a novel hepatitis E virus isolated from a patient with acute hepatitis reported in the United States. J Gen Virol 79:447–456

    PubMed  CAS  Google Scholar 

  20. Velázquez O, Stetler HC, Avila C, Ornelas G, Alvarez C, Hadler SC, Bradley DW, Sepúlveda J (1990) Epidemic transmission of enterically transmitted non-A, non-B hepatitis in Mexico, 1986–1987. JAMA 263:3281–3285

    Article  PubMed  Google Scholar 

  21. Vitral CL, Pinto MA, Lewis-Ximenez LL, Khudyakov YE, dos Santos DR, Gaspar AM (2005) Serological evidence of hepatitis E virus infection in different animal species from the Southeast of Brazil. Mem Inst Oswaldo Cruz 100:117–122

    Article  PubMed  Google Scholar 

  22. Wang QH, Han MG, Funk JA, Bowman G, Janies DA, Saif LJ (2005) Genetic diversity and recombination of porcine sapoviruses. J Clin Microbiol 43:5963–5972

    Article  PubMed  CAS  Google Scholar 

  23. Wang QH, Souza M, Funk JA, Zhang W, Saif LJ (2006) Prevalence of noroviruses and sapoviruses in swine of various ages determined by reverse transcription-PCR and microwell hybridization assays. J Clin Microbiol 44:2057–2062

    Article  PubMed  CAS  Google Scholar 

  24. Zheng Y, Ge S, Zhang J, Guo Q, Ng MH, Wang F, Xia N, Jiang Q (2006) Swine as a principal reservoir of hepatitis E virus that infects humans in Eastern China. J Infect Dis 193:1643–1649

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This study was partially supported by a grant from the National Veterinary Research and Quarantine Service, Korea.

Author information

Authors and Affiliations

  1. Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, South Korea

    J. N. Yu, M. Y. Kim, D. G. Kim, S. E. Kim, J. B. Lee, S. Y. Park, C. S. Song & I. S. Choi

  2. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, South Korea

    H. C. Shin

  3. Department of Public Health, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, South Korea

    K. H. Seo

Authors
  1. J. N. Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Y. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. G. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. E. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. B. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Y. Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. C. S. Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. H. C. Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. K. H. Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. I. S. Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. S. Choi.

Additional information

J. N. Yu and M. Y. Kim are equally contributed to this work

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, J.N., Kim, M.Y., Kim, D.G. et al. Prevalence of hepatitis E virus and sapovirus in post-weaning pigs and identification of their genetic diversity. Arch Virol 153, 739–742 (2008). https://doi.org/10.1007/s00705-008-0046-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-008-0046-5

Keywords

Search

Navigation