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Identification and full sequence of an isolate of Alternanthera mosaic potexvirus infecting Phlox stolonifera

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Summary.

A potexvirus was isolated from creeping phlox (Phlox stolonifera) plants from a commercial nursery in Pennsylvania. The virus was serologically related to clover yellow mosaic virus, plantain virus X, potato virus X, and potato aucuba mosaic virus, and was most closely related to papaya mosaic virus (PapMV). The sequence of a PCR fragment obtained with potexvirus group-specific primers was distinct from that of PapMV; the coat protein (CP) gene and 3′ untranslated region (UTR) were closely related to Alternanthera mosaic virus (AltMV), previously reported only from Australia. The host range was similar to that of the Australian isolate (AltMV-Au), and the phlox isolate reacted strongly with antiserum to AltMV-Au. The full sequence of the phlox isolate was more closely related to PapMV throughout the genome than to any potexvirus other than AltMV-Au, for which only the CP and 3′UTR sequences are available. The phlox isolate was therefore named AltMV-PA (for Pennsylvania), and the full 6607 nt sequence is presented1. Additional AltMV isolates from creeping phlox (AltMV-BR and AltMV-SP) and trailing portulaca (Portulaca grandiflora; AltMV-Po) were also isolated, suggesting that AltMV may be widespread, and may have been mis-diagnosed in the past as PapMV. AltMV has the potential to spread to other ornamental crops.

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References

  • MJ Adams JF Antoniw M Bar-Joseph AA Brunt T Candresse GD Foster GP Martelli RG Milne SK Zavriev CM Fauquet (2004) ArticleTitleThe new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch Virol 149 1045–1060 Occurrence Handle1:CAS:528:DC%2BD2cXjt1Sgtr4%3D Occurrence Handle15098118

    CAS  PubMed  Google Scholar 

  • SF Altschul TL Madden AA Schaffer J Zhang Z Zhang W Miller DJ Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25 3389–3402 Occurrence Handle10.1093/nar/25.17.3389 Occurrence Handle1:CAS:528:DyaK2sXlvFyhu7w%3D Occurrence Handle9254694

    Article  CAS  PubMed  Google Scholar 

  • Atabekov JG (1995) Genus Potexvirus. In: Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD (eds) Virus taxonomy: Sixth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York, pp 479–482

  • JB Bancroft R Rouleau R Johnston L Prins GA Mackie (1991) ArticleTitleThe entire nucleotide sequence of foxtail mosaic virus RNA. J Gen Virol 72 2173–2181 Occurrence Handle1:CAS:528:DyaK38XltVGgsro%3D Occurrence Handle1840610

    CAS  PubMed  Google Scholar 

  • S Chang J Puryear J Cairney (1993) ArticleTitleA simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Reptr 11 113–116 Occurrence Handle1:CAS:528:DyaK3sXlslKqurs%3D

    CAS  Google Scholar 

  • Christie RG, Edwardson JR (1977) Light and electron microscopy of plant virus inclusions. Fla Agric Exp Stn Monogr No. 9

  • M Ciuffo M Turina (2004) ArticleTitleA potexvirus related to Papaya mosaic virus isolated from moss rose (Portulaca grandiflora) in Italy. Plant Pathol 53 515 Occurrence Handle10.1111/j.1365-3059.2004.01040.x

    Article  Google Scholar 

  • VV Dolja VP Boyko AA Agranovsky EV Koonin (1991) ArticleTitlePhylogeny of capsid proteins of rod-shaped and filamentous RNA plant viruses: Two families with distinct patterns of sequence and probably structure conservation. Virology 184 79–86 Occurrence Handle1:STN:280:By6A3srnvFU%3D Occurrence Handle1871982

    CAS  PubMed  Google Scholar 

  • VV Dolja DP Grama D Morosov S Yu JG Atebekov (1987) ArticleTitlePotato virus X-related single and double-stranded RNAs. Characterization and identification of terminal structures. FEBS Lett 214 308–312

    Google Scholar 

  • Felsenstein J (1993) PHYLIP: Phylogeny Inference Package (ver 3.5c). Distributed by the author. Department of Genetics, University of Washington, Seattle

  • RLS Forster DL Beck PJ Guilford DM Voot CJ van Dolleweerd MT Andersen (1992) ArticleTitleThe coat protein of white clover mosaic potexvirus has a role in facilitating cell-to-cell transport in plants. Virology 191 480–484 Occurrence Handle10.1016/0042-6822(92)90215-B Occurrence Handle1:CAS:528:DyaK38Xmt12rsrw%3D Occurrence Handle1413520

    Article  CAS  PubMed  Google Scholar 

  • ADW Geering JE Thomas (1999) ArticleTitleCharacterization of a virus from Australia that is closely related to papaya mosaic potexvirus. Arch Virol 144 577–592 Occurrence Handle10.1007/s007050050526 Occurrence Handle1:CAS:528:DyaK1MXjs12rtL4%3D Occurrence Handle10226621

    Article  CAS  PubMed  Google Scholar 

  • D Güssow T Clackson (1989) ArticleTitleDirect clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res 17 4000 Occurrence Handle2734114

    PubMed  Google Scholar 

  • Hammond J (1990) Western blotting and the use of membranes to absorb antisera and to purify antibodies. In: Hampton RO, Ball EM, DeBoer S (eds) Serological detection and identification of plant viral and bacterial pathogens; A Laboratory Manual. APS Press, St. Paul, MN, pp 269–279

  • J Hammond R Hull (1981) ArticleTitlePlantain virus X: A new potexvirus from Plantago lanceolata. J Gen Virol 54 75–90 Occurrence Handle1:CAS:528:DyaL3MXksFOktLw%3D

    CAS  Google Scholar 

  • J Hammond RL Jordan RC Larsen JW Moyer (1992) ArticleTitleUse of polyclonal antisera and monoclonal antibodies to examine serological relationships among three filamentous viruses of sweetpotato. Phytopathology 82 713–717

    Google Scholar 

  • J Hammond RH Lawson (1988) ArticleTitleAn improved purification procedure for preparing potyviruses and cytoplasmic inclusion proteins from the same tissue. J Virol Methods 20 203–217 Occurrence Handle10.1016/0166-0934(88)90124-3 Occurrence Handle1:STN:280:BieA3Mrit1M%3D Occurrence Handle3410929

    Article  CAS  PubMed  Google Scholar 

  • J Hammond MD Reinsel CJM Maroon-Lango (2003) ArticleTitlePartial characterization and full sequence of a potexvirus from creeping phlox (Phlox stolonifera). Phytopathology 93 IssueID6 S32

    Google Scholar 

  • J Hammond MD Reinsel CJ Maroon-Lango (2004) ArticleTitleIdentification of potexvirus isolates from phlox and portulaca as strains of Alternanthera mosaic virus, and comparison of the genomic 3′ region. Phytopathology 94 IssueID6 S38

    Google Scholar 

  • Hammond J, Reinsel MD, Maroon-Lango CJ (2005) Identification of potexvirus isolates from creeping phlox and trailing portulaca as strains of Alternanthera mosaic virus, and comparison of the 3′-terminal portion of the viral genomes. Acta Hort (in press)

  • R Jordan J Hammond (1991) ArticleTitleComparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies. J Gen Virol 72 25–36 Occurrence Handle1703561

    PubMed  Google Scholar 

  • CP Joshi (1987) ArticleTitleAn inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucleic Acids Res 15 6643–6653 Occurrence Handle1:CAS:528:DyaL2sXlsVyntL0%3D Occurrence Handle3628002

    CAS  PubMed  Google Scholar 

  • M Kozak (1984) ArticleTitleCompilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res 12 857–872 Occurrence Handle1:CAS:528:DyaL2cXpslGkuw%3D%3D Occurrence Handle6694911

    CAS  PubMed  Google Scholar 

  • HA Lutcke KC Chow FS Mickel KA Moss HF Kern GA Scheele (1987) ArticleTitleSelection of AUG initiation codons differs in plants and animals. EMBO J 6 43–48 Occurrence Handle1:STN:280:BiiB3MfkslY%3D Occurrence Handle3556162

    CAS  PubMed  Google Scholar 

  • CJ Maroon-Lango MA Guaragna RL Jordan J Hammond M Bandla SK Marquardt (2005) ArticleTitleTwo unique US isolates of Pepino mosaic virus from a limited source of pooled tomato tissue are distinct from a third (European-like) US isolate. Arch Virol 150 1187–1201 Occurrence Handle10.1007/s00705-005-0495-z Occurrence Handle1:CAS:528:DC%2BD2MXkt1Ontrw%3D Occurrence Handle15750864

    Article  CAS  PubMed  Google Scholar 

  • SYu Morosov AG Solovyev (2003) ArticleTitleTriple gene block: modular design of a multifunctional machine for plant virus movement. J Gen Virol 84 1351–1366

    Google Scholar 

  • SYu Morosov NA Miroshnichenko AG Solovyev ON Fedorkin DA Zelenina LI Lukasheva AV Karasev VV Dolja JG Atabekov (1991) ArticleTitleExpression strategy of the potato virus X triple gene block. J Gen Virol 72 2039–2042

    Google Scholar 

  • E Myers W Miller (1989) ArticleTitleOptimal alignments in linear space. CABIOS 4 11–17

    Google Scholar 

  • RDM Page (1996) ArticleTitleTREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12 357–358 Occurrence Handle1:STN:280:ByiD2MfgtlA%3D Occurrence Handle8902363

    CAS  PubMed  Google Scholar 

  • Roche Molecular Biochemicals (2000) DIG Application Manual for Filter Hybridization. Mannheim, Germany

  • TL Sit MG AbouHaidar S Holy (1989) ArticleTitleNucleotide sequence of papaya mosaic virus RNA. J Gen Virol 70 2325–2331 Occurrence Handle1:CAS:528:DyaK3cXntFWjtg%3D%3D Occurrence Handle2778435

    CAS  PubMed  Google Scholar 

  • AG Solovyev EI Savenkov AA Agranovsky SYu Morosov (1996) ArticleTitleComparisons of the genomic cis-elements and coding regions in RNAβ components of the hordeiviruses Barley stripe mosaic virus, Lychnis ringspot virus, and Poa semi-latent virus. Virology 219 9–18 Occurrence Handle10.1006/viro.1996.0217 Occurrence Handle1:CAS:528:DyaK28XislCgu7o%3D Occurrence Handle8623558

    Article  CAS  PubMed  Google Scholar 

  • A Tamai T Meshi (2001) ArticleTitleCell-to-cell movement of Potato virus X: the role of p12 and p8 encoded by the second and third open reading frames of the triple gene block. Mol Plant-Microbe Interact 14 1158–1167 Occurrence Handle1:CAS:528:DC%2BD3MXnsVeltLo%3D Occurrence Handle11605955

    CAS  PubMed  Google Scholar 

  • JD Thompson DG Higgins TJ Gibson (1994) ArticleTitleCLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 4673–4680 Occurrence Handle1:CAS:528:DyaK2MXitlSgu74%3D Occurrence Handle7984417

    CAS  PubMed  Google Scholar 

  • J Verchot SM Angell DC Baulcombe (1998) ArticleTitleIn vivo translation of the triple gene block of potato virus X requires two subgenomic mRNAs. J Virol 72 8316–8320 Occurrence Handle1:CAS:528:DyaK1cXmt1Shuro%3D Occurrence Handle9733876

    CAS  PubMed  Google Scholar 

  • H Xu D Leclerc B Leung MG Abou Haidar (1994) ArticleTitleThe entire nucleotide sequence and genomic organization of potato aucuba mosaic potexvirus. Arch Virol 135 461–469 Occurrence Handle10.1007/BF01310031 Occurrence Handle1:CAS:528:DyaK2MXhsVegsw%3D%3D Occurrence Handle7979982

    Article  CAS  PubMed  Google Scholar 

  • Y Yamaji S Kagiwada H Nakabayashi M Ugaki S Namba (2001) ArticleTitleComplete nucleotide sequence of Tulip virus X (TVX-J): the border between species and strains within the genus Potexvirus. Arch Virol 146 2309–2320 Occurrence Handle10.1007/s007050170004 Occurrence Handle1:CAS:528:DC%2BD38Xjs12ks7k%3D Occurrence Handle11811681

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. U.S. Department of Agriculture, Agricultural Research Service, U.S. National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, Maryland, U.S.A.

    J. Hammond, M. D. Reinsel & C. J. Maroon-Lango

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  1. J. Hammond
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  2. M. D. Reinsel
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  3. C. J. Maroon-Lango
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Additional information

1The full sequence of AltMV-PA has been deposited in GenBank as Accession Number AY863024; the 3′-terminal sequences of AltMV-PA, AltMV-BR, AltMV-SP, and AltMV-Po have been deposited under Accession numbers AY850929, AY850928, AY850931, and AY850930, respectively.

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Hammond, J., Reinsel, M. & Maroon-Lango, C. Identification and full sequence of an isolate of Alternanthera mosaic potexvirus infecting Phlox stolonifera. Arch Virol 151, 477–493 (2006). https://doi.org/10.1007/s00705-005-0646-2

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