Skip to main content
SpringerLink
Log in

BOX-PCR fingerprinting for molecular typing of Salmonella enterica subsp. enterica serovar Enteritidis strains originated from humans, dairy cattle, and broiler chickens

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

Salmonellosis is a global zoonotic infection of domestic animals and poultry with serious consequences on public health. Globally one of the most prevalent and common Salmonella enterica serotypes is serovar Enteritidis. Our goal in this research was a molecular characterization and evaluation of biodiversity and relatedness of different Salmonella isolates. Sixty-four Salmonella Enteritidis isolates which were gathered and cultured from various hosts and locations including dairy cows, broiler chicken, and patients with diarrhea of Tehran and Kerman provinces were typed by BOX-PCR assay. Dendrograms were produced by NTSYS computer software version 2.0. PCR products with different sizes from 250 to 3000 bps and the number of 5–14 bands visualized by agarose gel electrophoresis and 28 molecular patterns detected. By cluster analysis, chicken and human isolates were more similar (77.6%) than dairy cow isolates (44%). The results showed that the BOX-PCR assay is a beneficial and applicative molecular method with high discriminatory power (92.91%) for typing of the Salmonella strains and detection of heterogeneity and genetic diversity (90.52%) calculated by Shannon-Wiener index.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Albufera U, Bhugaloo-Vial P, Issack M, Jaufeerally-Fakim Y (2009) Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis. Infect Genet Evol 9:322–327

    Article  CAS  PubMed  Google Scholar 

  • Amini K, Zahraei-Salehi T, Nikbakht Gh, Ranjbar R, Amini J, Ashrafganjooei SB (2010) Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from human and animals in Iran. Afr J Microbiol Res 4(21):2202–2210

  • Andrews-Polymenis HL, Santiviago CA, McClelland M (2009) Novel genetic tools for studying food-borne Salmonella. Curr Opin Biotechnol 20:149–157

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bhowmick PP, Srikumar S, Devegowda D, Shekar M, Ruwandeepika HD, Karunasagar I (2012) Serotyping & molecular characterization for study of genetic diversity among seafood associated nontyphoidal Salmonella serovars. Indian J Med Res 135:371

    PubMed  PubMed Central  Google Scholar 

  • Burr M, Josephson K, Pepper I (1998) An evaluation of ERIC PCR and AP PCR fingerprinting for discriminating Salmonella serotypes. Lett Appl Microbiol 27:24–30

    Article  CAS  PubMed  Google Scholar 

  • Chmielewski R, Wieliczko A, Kuczkowski M, Mazurkiewicz M, Ugorski M (2002) Comparison of ITS profiling, REP-and ERIC-PCR of Salmonella Enteritidis isolates from Poland. J Veterinary Med Ser B 49:163–168

    Article  CAS  Google Scholar 

  • de Oliveira SD, Bessa MC, dos Santos LR, de Itapema Cardoso MR, Brandelli A, Canal CW (2007) Phenotypic and genotypic characterization of Salmonella Enteritidis isolates. BJM 38:720–728

    Google Scholar 

  • Guibourdenche M, Roggentin P, Mikoleit M, Fields PI, Bockemühl J, Grimont PA, Weill F-X (2010) Supplement 2003–2007 (No. 47) to the white-Kauffmann-Le minor scheme. Res Microbiol 161:26–29

    Article  PubMed  Google Scholar 

  • Hunter PR, Gaston MA (1988) Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol 26:2465–2466

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ishii S, Sadowsky MJ (2009) Applications of the rep-PCR DNA fingerprinting technique to study microbial diversity, ecology and evolution. Environ Microbiol 11:733–740

    Article  CAS  PubMed  Google Scholar 

  • Johnson JR, Clabots C (2000) Improved repetitive-element PCR fingerprinting of Salmonella enterica with the use of extremely elevated annealing temperatures. Clin Diagn Lab Immunol 7:258–264

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lee CM, Sieo CC, Cheah YK, Abdullah N, Ho YW (2012) Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced-based PCR fingerprinting. J Sci Food Agric 92:660–666

    Article  CAS  PubMed  Google Scholar 

  • Lupski JR, Weinstock GM (1992) Short, interspersed repetitive DNA sequences in prokaryotic genomes. J Bacteriol 174:4525–4529

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mohapatra BR, Broersma K, Mazumder A (2007) Comparison of five rep-PCR genomic fingerprinting methods for differentiation of fecal Escherichia coli from humans, poultry and wild birds. FEMS Microbiol Lett 277:98–106

    Article  CAS  PubMed  Google Scholar 

  • Olson AB, Andrysiak AK, Tracz DM, Guard-Bouldin J, Demczuk W, Ng L-K, Maki A, Jamieson F, Gilmour MW (2007) Limited genetic diversity in Salmonella enterica serovar Enteritidis PT13. BMC Microbiol 7:87

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Porwollik S, Wong RM, McClelland M (2002) Evolutionary genomics of Salmonella: gene acquisitions revealed by microarray analysis. Proc Natl Acad Sci USA 99(13):8956–8961

  • Rasschaert G, Houf K, Imberechts H, Grijspeerdt K, De Zutter L, Heyndrickx M (2005) Comparison of five repetitive-sequence-based PCR typing methods for molecular discrimination of Salmonella enterica isolates. JCM 43:3615–3623

    Article  CAS  Google Scholar 

  • Smith SI, Fowora MA, Goodluck HA, Nwaokorie FO, Aboaba OO, Opere B (2011) Molecular typing of Salmonella spp isolated from food handlers and animals in Nigeria. IJMEG 2:73

    CAS  PubMed  Google Scholar 

  • Suh DK, Song JC (2006) Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles. J Vet Sci 7:37–41

    Article  PubMed  PubMed Central  Google Scholar 

  • Ussery DW, Hallin PF (2004) Genome update: annotation quality in sequenced microbial genomes. J Microbiol 150:2015–2017

    Article  CAS  Google Scholar 

  • Versalovic J, Koeuth T, Lupski R (1991) Distribution of repetitive DNA sequences in eubacteria and application to finerpriting of bacterial enomes. Nucleic Acids Res 19:6823–6831

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Versalovic J, Schneider M, De Bruijn F, Lupski JR (1994) Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40

    CAS  Google Scholar 

  • Weigel RM, Qiao B, Teferedegne B, Suh DK, Barber DA, Isaacson RE, White BA (2004) Comparison of pulsed field gel electrophoresis and repetitive sequence polymerase chain reaction as genotyping methods for detection of genetic diversity and inferring transmission of Salmonella. Vet Microbiol 100:205–217

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We appreciate Dr. Ali Masoudi-Nejad, Associate Professor of Systems Biology & Bioinformatics of Tehran University, for his help in data analysis and preparation of the dendrograms.

Funding

This study was funded by the School of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran. This study was supported by a grant from college of veterinary medicine, Islamic Azad University, Tehran Science and Research Branch, Tehran, Iran.

Author information

Authors and Affiliations

  1. Department of Microbiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Abbas Ali Shiroodi, Mahmood Jamshidian & Taghi Zahraei Salehi

  2. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Gholam Reza Nikbakht Boroujeni

  3. Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran

    Kumarss Amini

Authors
  1. Abbas Ali Shiroodi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mahmood Jamshidian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Taghi Zahraei Salehi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gholam Reza Nikbakht Boroujeni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kumarss Amini
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Taghi Zahraei Salehi.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ali Shiroodi, A., Jamshidian, M., Salehi, T.Z. et al. BOX-PCR fingerprinting for molecular typing of Salmonella enterica subsp. enterica serovar Enteritidis strains originated from humans, dairy cattle, and broiler chickens. Comp Clin Pathol 28, 679–683 (2019). https://doi.org/10.1007/s00580-019-02897-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-019-02897-z

Keywords

Search

Navigation