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Regulatory volume decrease in northern fur seal (Callorhinus ursinus) red blood cells

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Abstract

The cation transport and regulatory volume decrease (RVD) were investigated in the red blood cells (RBCs) of northern fur seals (Callorhinus ursinus). Extracellular Ca-dependent Na efflux was increased to threefold by hypotonicity. K–Cl cotransport activity was not detected by hypotonic medium, but measured only by nitrite or N-ethylmaleimide stimulation. RBCs were restored to their original volume after being swollen in hypoosmotic medium with Ca, though this recovery was inhibited by the addition of quinidine. Based on these results, Na/Ca exchange transporter played the major role in the regulatory volume decrease in the RBCs of northern fur seals.

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Acknowledgement

The technical assistance of T. Fukuoka and K. Nishiki is gratefully acknowledged.

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Authors and Affiliations

  1. Research Institute of Biosciences, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa, 229-8501, Japan

    H. Ochiai

  2. Laboratory of Pathobiochemistry, School of Veterinary Medicine, Azabu University, Fuchinobe, Sagamihara, Kanagawa, 229-8501, Japan

    N. Hishiyama, K. Higa & H. Fujise

  3. Izu-Mito Sea Paradise, 3-1 Uchiura, Numazu, Shizuoka, 410-0223, Japan

    K. Koyama

  4. National Research Institute of Far Seas Fisheries, Fisheries Research Agency, 5-7-1 Orido, Shizuoka, Shizuoka, 424-8633, Japan

    M. Seita

Authors
  1. H. Ochiai
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  2. N. Hishiyama
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  3. K. Higa
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  4. K. Koyama
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  5. M. Seita
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  6. H. Fujise
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Corresponding author

Correspondence to H. Ochiai.

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H. Fujise has passed away.

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Ochiai, H., Hishiyama, N., Higa, K. et al. Regulatory volume decrease in northern fur seal (Callorhinus ursinus) red blood cells. Comp Clin Pathol 16, 61–63 (2007). https://doi.org/10.1007/s00580-006-0643-1

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  • DOI: https://doi.org/10.1007/s00580-006-0643-1

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