Skip to main content
SpringerLink
Log in

Effects of surfactant on lung injury induced by hyperoxia and mechanical ventilation in rabbits

  • Original Articles
  • Published:
Journal of Anesthesia Aims and scope Submit manuscript

Abstract

We evaluated the effects of exogenous surfactant on lung injury caused by 100% oxygen and mechanical ventilation in rabbits. Surfactant-treated rabbits (n=9) were ventilated with 100% oxygen for 36 hours and bovine surfactant was given via the trachea 12 hours after the start of mechanical ventilation. Saline-treated (n=9) rabbits were treated identically, except that they received saline without surfactant. There were no significant changes in hemodynamics, lung mechanics, or arterial oxygen tension during artificial ventilation.

Albumin concentration in the bronchoalveolar lavage fluid (BALF) of saline-treated rabbits was slightly higher than those in surfactanttreated rabbits and significantly higher than in non-treated rabbits. C3a concentration in BALF was significantly higher in saline-treated rabbits than in surfactant-treated and non-treated rabbits. In addition, the wet-to-dry lung weight ratio was significantly lower in surfactanttreated rabbits than in saline-treated rabbits (5.06±0.10 vs. 5.67±0.14,P<0.05).

Light microscopy revealed hyaline membrane formation in saline-, treated rabbits, but fewer changes were observed in surfactant-treated rabbits. Electron microscopy revealed extensive endothelial cell destruction in saline-treated rabbits, while such changes except endothelial cell swelling were not observed in surfactant-treated rabbits.

We conclude that exogenous surfactant attenuated lung injury caused by oxygen exposure and ventilation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Klein J: Normobaric pulmonary oxygen toxicity, Anesth Analg 70:195–207, 1990

    Article  CAS  PubMed  Google Scholar 

  2. Clark JM, Lambertsen CJ: Pulmonary oxygen toxicity. A review. Pharmacol Rev 23:37–133, 1971

    CAS  PubMed  Google Scholar 

  3. Kistler GS, Caldwell PRB, Weibel ER: Development of fine structural damage to alveolar and capillary lining cells in oxygen-poisoned rat lung. J Cell BioI 32:605–628, 1967

    Article  CAS  Google Scholar 

  4. Obara H, Hoshino Y, Mori M, et al: Endothelium-dependent relaxation in isolated pulmonary arteries from rabbits exposed to hyperoxia. Crit Care Med 17:780–785, 1989

    Article  CAS  PubMed  Google Scholar 

  5. Obara H, Sekimoto M, Iwai S: Alterations to the bronchial and bronchiolar surfaces of adult mice after exposure to high concentrations of oxygen. Thorax 34:479–485, 1979

    Article  CAS  PubMed  Google Scholar 

  6. Crapo JD: Morphologic changes in pulmonary oxygen toxicity. Annu Rev Physiol 48:721–731, 1986

    Article  CAS  PubMed  Google Scholar 

  7. Matalon S, Holm BA, Notter RH: Mitigation of pulmonary hyperoxic injury by administration of exogenous surfactant. J Appl Physiol 62:756–761, 1987

    CAS  PubMed  Google Scholar 

  8. Loewen GM, Holm BA, Milanowski L, et al: Alveolar hyperoxic injury in rabbits receiving exogenous surfactant. J Appl Physiol 66:1087–1092, 1989

    CAS  PubMed  Google Scholar 

  9. Crapo JD, Barry BE, Foscue HA, et al: Structural and biochemical changes in rat lungs occurring during exposures to lethal and adaptive doses of oxygen. Am Rev Respir Dis 122:123–143, 1980

    CAS  PubMed  Google Scholar 

  10. Fox RB, Hoidal JR, Brown DM, et al: Pulmonary inflammation due to oxygen toxicity: Involvement of chemotactic factors and polymorphonuclear leukocytes. Am Rev Respir Dis 123:521–523, 1981

    CAS  PubMed  Google Scholar 

  11. Barry BE, Crapo JD: Pattern of accumulation of platelets and neutrophils in rat lungs during exposure to 100% and 85% oxygen. Am Rev Respir Dis 132:548–555, 1985

    CAS  PubMed  Google Scholar 

  12. Rinaldo JE, English D, Levine J, et al: Increased intrapulmonary retension of radiolabeled neutrophils in early oxygen toxicity. Am Rev Respir Dis 137:345–352, 1988

    CAS  PubMed  Google Scholar 

  13. LeSouf PN, England SJ, Bryan AC: Total resistance of the respiratory system in preterm infants with and without an endotracheal tube. J Pediatr 104:108–111, 1984

    Article  Google Scholar 

  14. Takayama M, Itoh S, Nagasaki T, et al: A new enzymatic method for determination of serum cholinecontaining phospholipids. Clin Chim Acta 79:93–98, 1977

    Article  CAS  PubMed  Google Scholar 

  15. de los Santos R, Seidenfeld JJ, Anzueto A, et al: One hundred percent oxygen lung injury in adult baboons. Am Rev Respir Dis 136:657–661, 1987

    PubMed  Google Scholar 

  16. Davis WB, Stephen I, Rennard SI, et al: Pulmonary oxygen toxicity. Early reversible changes in human alveolar structures induced by hyperoxia. N Engl J Med 309:878–883, 1983

    Article  CAS  PubMed  Google Scholar 

  17. Matalon S, Cesar MA: Effects of 100% oxygen breathing on the capillary filtration coefficient in rabbit lungs. Microvasc Res 29:70–80, 1985

    Article  CAS  PubMed  Google Scholar 

  18. Dreyfuss D, Soler P, Basset G, et al: High inflation pressure pulmonary edema. Am Rev Respir Dis 137:1159–1164, 1988

    CAS  PubMed  Google Scholar 

  19. Kolobow T, Moretti MP, Fumagalli R, et al: Severe impairment in function induced by high peak airway pressure during mechanical ventilation. Am Rev Respir Dis 135:312–315, 1987

    CAS  PubMed  Google Scholar 

  20. Gross NJ, Smith DM: Impaired surfactant phospholipid metabolism in hyperoxic mouse lungs. J Appl Physiol 51:1198–1203, 1981

    CAS  PubMed  Google Scholar 

  21. Holm BA, Matalon S, Finkelstein IN, et al: Type II pneumocyte changes during hyperoxic lung injury and recovery. J Appl Physiol 65:2672–2678, 1988

    CAS  PubMed  Google Scholar 

  22. Kobayashi T, Nitta K, Ganzuka M, et al: Inactivation of exogenous surfactant by pulmonary edema fluid. Pediatr Res 29:353–356, 1991

    Article  CAS  PubMed  Google Scholar 

  23. Fuchimukai T, Fujiwara T, Takahashi A, et al: Artificial pulmonary surfactant inhibited by proteins. J Appl Physiol 62:429–437, 1987

    Article  CAS  PubMed  Google Scholar 

  24. Seeger W, Stöhr G, Wolf HRD, et al: Alteration of surfactant function due to protein leakage: special interaction with fibrin monomer. J Appl Physiol 58:326–338, 1985

    CAS  PubMed  Google Scholar 

  25. Holm BA, Enhorning G, Notter RH: A biophysical mechanism by which plasma proteins inhibit lung surfactant activity. Chern Phys Lipids 49:49–55, 1988

    Article  CAS  Google Scholar 

  26. Jobe A, Ikegami M, Jacobs H, et al: Permeability of premature lamb lungs to protein and the effect of surfactant on that permeability. J Appl Physiol 55:169–176,1983

    CAS  PubMed  Google Scholar 

  27. Hills BA: Water repellency induced by pulmonary surfact.ants. J Physiol 325:175–186, 1982

    CAS  PubMed  Google Scholar 

  28. Hills BA: ‘De-watering’ capability of surfactants in human amniotic fluid. J Physiol 348:369–381, 1984

    CAS  PubMed  Google Scholar 

  29. Fridovich I: The biology of oxygen radicals. Science 201:875–880, 1978

    Article  CAS  PubMed  Google Scholar 

  30. Halliwell B, Gutteridge JMC: Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219:1–14, 1984

    CAS  PubMed  Google Scholar 

  31. Buckley BJ, Tanswell AK, Freeman BR: Liposome-mediated augmentation of catalase in alveolar type II cells protects against H202 injury. J Appl Physiol 63:359–367, 1987

    CAS  PubMed  Google Scholar 

  32. Tanswell AK, Freeman BA: Liposomeentrapped antioxidant enzymes prevent lethal O2 toxicity in the newborn rat. J Appl Physiol 63:347–352, 1987

    CAS  PubMed  Google Scholar 

  33. Turrens JF, Crapo JD, Freeman BA: Protection against oxygen toxicity by intravenous injection of liposomeentrapped catalase and superoxide dismutase. J Clin Invest 73:87–95, 1984

    Article  CAS  PubMed  Google Scholar 

  34. Matalon S, Holm BA, Baker RR, et al: Antioxidant properties of surfactant replacement mixtures. Am Rev Respir Dis 137:80, 1988

    Google Scholar 

  35. Hayakawa H, Myrvik QN, Clair RWST: Pulmonary surfactant inhibits priming of rabbit alveolar macrophage. Evidence that surfactant suppress the oxidative burst of alveolar macrophage in infant rabbit. Am Rev Respir Dis 140:1390–1397, 1989

    CAS  PubMed  Google Scholar 

  36. Parsons PE, Fowler AA, Hyers TM, et al: Chemotactic activity in bronchoalveolar lavage fluid from patients with adult respiratory distress syndrome. Am Rev Respir Dis 132:490–493, 1985

    CAS  PubMed  Google Scholar 

  37. Merritt TA, Hallman M, Holcomb K, et al: Human surfactant treatment of severe respiratory distress syndrome: Pulmonary effluent indicators of lung inflammation. J Pediatr 108:741–748, 1986

    Article  CAS  PubMed  Google Scholar 

  38. Damiano VV, Cohen A, Tsang A, et al: A morphologic study of the influx of neutrophils into dog lung alveoli after lavage with sterile saline. Am J Pathoi 100:349–364, 1980

    CAS  Google Scholar 

  39. Kwong MS, Eagan EA, Notter RH: A double blind clinical trial of calf lung lipid for the prevention of hyaline membrane disease in extremely premature infants. Pediatrics 76:585–592, 1985

    CAS  PubMed  Google Scholar 

  40. Fujiwara T, Maeta J, Chida S, et al: Artifical surfactant for treatment of hyaline-membrane disease. Lancet 1:55–59, 1980

    Article  CAS  PubMed  Google Scholar 

  41. Maeta H, Raju TNK, Vidyasagar D, et al: Effect of exogenous surfactant on the development of bronchopulmonary dysplasia in a baboon hyaline membrane disease model. Crit Care Med 18:403–409, 1990

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anesthesiology, Kobe University School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, 650, Kobe, Japan

    Junichi Ikegaki, Katsuya Mikawa & Hidefumi Obara

Authors
  1. Junichi Ikegaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Katsuya Mikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hidefumi Obara
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Ikegaki, J., Mikawa, K. & Obara, H. Effects of surfactant on lung injury induced by hyperoxia and mechanical ventilation in rabbits. J Anesth 7, 66–74 (1993). https://doi.org/10.1007/s0054030070066

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s0054030070066

Key words

Search

Navigation