Abstract
Purpose
Gallbladder cancer (GBC), the most common gastrointestinal and biliary tract malignancy, often coincides with gallstone disease (GSD). The genetic variants of adrenergic receptor (ADR) have been previously reported to be associated with hypomotility disorder of cardiovascular system and GSD. Since GSD may function as GBC precursor, the present study aimed to investigate the association of common functional genetic variants of ADRA2A C-1291G, ADRβ3 T190C or Trp64Arg, and ADRβ1 C1165G or Arg389Gly with GBC and GSD susceptibility.
Methods
The present study included a total of 400 histologically confirmed GBC, 230 GSD, and 268 healthy controls. The ADRA2A C-1291G, ADRβ3 T190C, and ADRβ1 C1165G polymorphisms were determined by PCR–RFLP. Statistical analysis was performed by using SPSS version 16.
Results
ADRβ3 T190C polymorphism was significantly associated with increased risk of GBC (CT: Pcorr = 0.015, OR 2.87; CC: Pcorr = 0.038, OR 10.33; C allele: Pcorr = 0.014, OR 2.7; CT + CC: Pcorr = 0.017, OR 3.05). These associations existed even after gallstone and gender stratification. Similarly, ADRβ3 T190C polymorphism was also associated with GSD risk, though limited only to female GSD patients. In contrary, ADRA2A C-1291G conferred a marginally increased risk only in GSD patients. ADRβ1 C1165G polymorphism was not associated with GBC and GSD susceptibility when compared to controls.
Conclusion
ADRβ3 T190C polymorphism is significantly associated with GBC and GSD susceptibility. The ADRβ3 T190C may be involved in the pathophysiology of GBC by both gallstone-dependent pathway and by some other independent mechanisms.
Similar content being viewed by others
Abbreviations
- GBC:
-
Gallbladder cancer
- ADR:
-
Adrenergic receptors
- HC:
-
Healthy controls
- GSD:
-
Gallstone disease
- SNP:
-
Single-nucleotide polymorphism
- SPSS:
-
Statistical package for social sciences
- FDR:
-
False discovery rate
- OR:
-
Odds ratio
- CI:
-
Confidence interval
- HWE:
-
Hardy–Weinberg equilibrium
References
Anthony A, Schepelmann S, Guillaume JL et al (1998) Localization of the β(beta)3-adrenoceptor in the human gastrointestinal tract: an immunohistochemical study. Aliment Pharmacol Ther 12:519–525
Babol K, Blasiak J (2005) Beta3-adrenergic receptor. Postepy Biochem 51:80–87
Babol K, Przybylowska K, Lukaszek M, Pertynski T, Blasiak J (2004) An association between the Trp64Arg polymorphism in the beta3-adrenergic receptor gene and endometrial cancer and obesity. J Exp Clin Cancer Res 23:669–674
Bracale R, Pasanisi F, Labruna G et al (2007) Metabolic syndrome and ADRB3 gene polymorphism in severely obese patients from South Italy. Eur J Clin Nutr 61:1213–1219
Bristow MR, Ginsburg R, Umans V et al (1986) Beta 1- and beta 2-adrenergic-receptor subpopulations in nonfailing and failing human ventricular myocardium: coupling of both receptor subtypes to muscle contraction and selective beta 1-receptor down-regulation in heart failure. Circ Res 59:297–309
Bylund DB (1992) Subtypes of alpha 1- and alpha 2-adrenergic receptors. FASEB J 6:832–839
Chou YC, Tsai CN, Lee YS, Pei JS (2012) Association of adrenergic receptor gene polymorphisms with adolescent obesity in Taiwan. Pediatr Int 54:111–116
Chuang SC, Hsi E, Lee KT (2013) Genetics of gallstone disease. Adv Clin Chem 60:143–185
Cole SW, Sood AK (2012) Molecular pathways: beta-adrenergic signaling in cancer. Clin Cancer Res 18:1201–1206
Doggrell SA, Scott GW (1980) The occurrence of postsynaptic alpha- and beta-adrenoceptors in the guinea-pig gall bladder. Br J Pharmacol 71:185–189
Everhart JE (1993) Contributions of obesity and weight loss to gallstone disease. Ann Intern Med 119:1029–1035
Forray C, Bard JA, Wetzel JM et al (1994) The alpha 1-adrenergic receptor that mediates smooth muscle contraction in human prostate has the pharmacological properties of the cloned human alpha 1c subtype. Mol Pharmacol 45:703–708
Guillaume JL, Petitjean F, Haasemann M, Bianchi C, Eshdat Y, Strosberg AD (1994) Antibodies for the immunochemistry of the human beta 3-adrenergic receptor. Eur J Biochem 224:761–770
Hamdani NH, Qadri SK, Aggarwalla R et al (2012) Clinicopathological study of gall bladder carcinoma with special reference to gallstones: our 8-year experience from eastern India. Asian Pac J Cancer Prev 13:5613–5617
Hegele RA, Harris SB, Hanley AJ, Azouz H, Connelly PW, Zinman B (1998) Absence of association between genetic variation of the beta 3-adrenergic receptor and metabolic phenotypes in Oji-Cree. Diabetes Care 21:851–854
Huang XE, Hamajima N, Saito T et al (2001) Possible association of beta2- and beta3-adrenergic receptor gene polymorphisms with susceptibility to breast cancer. Breast Cancer Res 3:264–269
Kimura K, Sasaki N, Asano A et al (2000) Mutated human beta3-adrenergic receptor (Trp64Arg) lowers the response to beta3-adrenergic agonists in transfected 3T3-L1 preadipocytes. Horm Metab Res 32:91–96
Klass DM, Lauer N, Hay B, Kratzer W, Fuchs M (2007) Arg64 variant of the beta3-adrenergic receptor is associated with gallstone formation. Am J Gastroenterol 102:2482–2487
Kobilka B (1992) Adrenergic receptors as models for G protein-coupled receptors. Annu Rev Neurosci 15:87–114
Krief S, Lonnqvist F, Raimbault S et al (1993) Tissue distribution of beta 3-adrenergic receptor mRNA in man. J Clin Invest 91:344–349
Kumar S, Mishra A, Srivastava A, Mittal T, Garg N, Mittal B (2014) Significant role of ADRB3 rs4994 towards the development of coronary artery disease. Coron Artery Dis 25:29–34
Lima JJ, Feng H, Duckworth L et al (2007) Association analyses of adrenergic receptor polymorphisms with obesity and metabolic alterations. Metabolism 56:757–765
Liu ZQ, Mo W, Huang Q, Zhou HH (2007) Genetic polymorphisms of human beta-adrenergic receptor genes and their association with obesity. Zhong Nan Da Xue Xue Bao Yi Xue Ban 32:359–367
Liu WN, Fu KL, Gao HY et al (2012) beta1 adrenergic receptor polymorphisms and heart failure: a meta-analysis on susceptibility, response to beta-blocker therapy and prognosis. PLoS ONE 7:e37659
Mathur SK, Duhan A, Singh S et al (2012) Correlation of gallstone characteristics with mucosal changes in gall bladder. Trop Gastroenterol 33:39–44
Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:1215
Misra S, Chaturvedi A, Misra NC, Sharma ID (2003) Carcinoma of the gallbladder. Lancet Oncol 4:167–176
Oguri K, Tachi T, Matsuoka T (2013) Visceral fat accumulation and metabolic syndrome in children: the impact of Trp64Arg polymorphism of the beta3-adrenergic receptor gene. Acta Paediatr 102:613–619
Parkinson NA, Hughes AD (1995) The mechanism of action of alpha 2-adrenoceptors in human isolated subcutaneous resistance arteries. Br J Pharmacol 115:1463–1468
Plummer HK, Yu Q, Cakir Y, Schuller HM (2004) Expression of inwardly rectifying potassium channels (GIRKs) and beta-adrenergic regulation of breast cancer cell lines. BMC Cancer 4:93
Quoc Lu’o’ng KV, Nguyen LT (2012) The roles of beta-adrenergic receptors in tumorigenesis and the possible use of beta-adrenergic blockers for cancer treatment: possible genetic and cell-signalling mechanisms. Cancer Manag Res 4:431–445
Rai R, Tewari M, Kumar M, Singh TB, Shukla HS (2011) Expression profile of cholecystokinin type-A receptor in gallbladder cancer and gallstone disease. Hepatobiliary Pancreat Dis Int 10:408–414
Rai R, Sharma KL, Misra S, Kumar A, Mittal B (2013) PSCA gene variants (rs2294008 and rs2978974) confer increased susceptibility of gallbladder carcinoma in females. Gene 530:172–177
Rosmond R, Bouchard C, Bjorntorp P (2002) A C-1291G polymorphism in the alpha2A-adrenergic receptor gene (ADRA2A) promoter is associated with cortisol escape from dexamethasone and elevated glucose levels. J Intern Med 251:252–257
Rudner XL, Berkowitz DE, Booth JV et al (1999) Subtype specific regulation of human vascular alpha(1)-adrenergic receptors by vessel bed and age. Circulation 100:2336–2343
Sahlin S, Granstrom L, Gustafsson U, Stahlberg D, Backman L, Einarsson K (1994) Hepatic esterification rate of cholesterol and biliary lipids in human obesity. J Lipid Res 35:484–490
Schlesinger S, Aleksandrova K, Pischon T et al (2013) Abdominal obesity, weight gain during adulthood and risk of liver and biliary tract cancer in a European cohort. Int J Cancer 132:645–657
Seremak-Mrozikiewicz A, Drews K, Nowocien G, Kaluba-Skotarczak A, Boc A (2008) The significance of beta-3 adrenergic receptor polymorphism in excessive body gain and obesity development in pregnant women. Ginekol Pol 79:51–55
Srivastava A, Pandey SN, Dixit M, Choudhuri G, Mittal B (2008) Cholecystokinin receptor A gene polymorphism in gallstone disease and gallbladder cancer. J Gastroenterol Hepatol 23:970–975
Srivastava A, Mishra A, Singh R, Rai R, Srivastava N, Mittal B (2013) Multi-analytic approach elucidates significant role of hormonal and hepatocanalicular transporter genetic variants in gallstone disease in North Indian population. PLoS ONE 8:e59173
Strosberg AD (1993) Structure, function, and regulation of adrenergic receptors. Protein Sci 2:1198–1209
Takeuchi S, Katoh T, Yamauchi T, Kuroda Y (2012) ADRB3 polymorphism associated with BMI gain in Japanese men. Exp Diabetes Res 2012:973561
Takezaki T, Hamajima N, Matsuo K et al (2001) Association of polymorphisms in the beta-2 and beta-3 adrenoceptor genes with risk of colorectal cancer in Japanese. Int J Clin Oncol 6:117–122
Tsuchiya Y, Terao M, Okano K et al (2011) Mutagenicity and mutagens of the red chilli pepper as gallbladder cancer risk factor in Chilean women. Asian Pac J Cancer Prev 12:471–476
Walston J, Silver K, Bogardus C et al (1995) Time of onset of non-insulin-dependent diabetes mellitus and genetic variation in the beta 3-adrenergic-receptor gene. N Engl J Med 333:343–347
Wang H, Liu J, Liu K et al (2013) Beta1-adrenoceptor gene Arg389Gly polymorphism and essential hypertension risk in general population: a meta-analysis. Mol Biol Rep 40:4055–4063
Wenjuan Y, Yujun L, Ceng Y (2013) Association of single nucleotide polymorphisms of beta2-adrenergic receptor gene with clinicopathological features of pancreatic carcinoma. Acta Histochem 115:198–203
Wistuba II, Gazdar AF (2004) Gallbladder cancer: lessons from a rare tumour. Nat Rev Cancer 4:695–706
Xu HL, Hsing AW, Vogtmann E et al (2013) Variants in CCK and CCKAR genes to susceptibility to biliary tract cancers and stones: a population-based study in Shanghai, China. J Gastroenterol Hepatol 28:1476–1481
Acknowledgments
The authors thank Department of Biotechnology and Indian Council of Medical Research, Govt of India, for financial support to carry out the present study.
Conflict of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Rai, R., Sharma, K.L., Misra, S. et al. Association of adrenergic receptor gene polymorphisms in gallbladder cancer susceptibility in a North Indian population. J Cancer Res Clin Oncol 140, 725–735 (2014). https://doi.org/10.1007/s00432-014-1621-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00432-014-1621-7