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ALY as a potential contributor to metastasis in human oral squamous cell carcinoma

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Abstract

Purpose

ALY, an essential mRNA export factor, is dysregulated in a wide variety of human malignancies. However, little is known about the relevance of ALY to oral squamous cell carcinoma (OSCC). The purpose of this study was to investigate ALY expression and its functional mechanisms in OSCCs.

Methods

ALY mRNA and protein expression in seven OSCC-derived cell lines (Sa3, HO-1-u-1, KON, Ca9-22, HSC-2, HSC-3, and HSC-4) and primary OSCCs were analyzed by quantitative reverse transcriptase-polymerase chain reaction, immunoblotting, and immunohistochemistry. We evaluated cellular invasiveness, migration, and the expression levels of metastasis modulators, ribosomal RNA processing 1 homolog B (RRP1B) and CD82, in ALY knockdown cells.

Results

ALY was frequently up-regulated in OSCC-derived cell lines and primary OSCCs compared with normal counterparts at both the mRNA and protein expression levels. ALY-positive expression was correlated significantly (P < 0.05) with a higher risk of regional lymph node metastasis. Furthermore, ALY knockdown cells caused a significant (P < 0.05) decrease in cellular invasiveness and migration with up-regulation of RRP1B and CD82 compared with the control cells.

Conclusion

Our results showed that ALY is linked to regional lymph node metastasis by regulating cellular invasiveness and migration. Therefore, ALY might be a potential biomarker for early detection of lymph node metastasis in OSCCs.

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Acknowledgments

We thank Lynda C. Charters for editing this manuscript.

Conflict of interest

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Authors and Affiliations

  1. Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Yasuhiro Saito, Atsushi Kasamatsu, Ayumi Yamamoto, Toshihiro Shimizu, Hideki Tanzawa & Katsuhiro Uzawa

  2. Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Yosuke Sakamoto, Katsunori Ogawara, Masashi Shiiba, Hideki Tanzawa & Katsuhiro Uzawa

  3. Department of Oral and Maxillofacial Surgery Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

    Hidetaka Yokoe

Authors
  1. Yasuhiro Saito
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  2. Atsushi Kasamatsu
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  3. Ayumi Yamamoto
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  4. Toshihiro Shimizu
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  5. Hidetaka Yokoe
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  6. Yosuke Sakamoto
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  7. Katsunori Ogawara
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  8. Masashi Shiiba
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  9. Hideki Tanzawa
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  10. Katsuhiro Uzawa
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Corresponding authors

Correspondence to Atsushi Kasamatsu or Katsuhiro Uzawa.

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Saito, Y., Kasamatsu, A., Yamamoto, A. et al. ALY as a potential contributor to metastasis in human oral squamous cell carcinoma. J Cancer Res Clin Oncol 139, 585–594 (2013). https://doi.org/10.1007/s00432-012-1361-5

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  • DOI: https://doi.org/10.1007/s00432-012-1361-5

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