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Proteome analysis of Arabidopsis seedlings exposed to bacterial volatiles

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Abstract

Plant root-associated bacteria (rhizobacteria) elicit plant basal immunity referred to as induced systemic resistance (ISR) against multiple pathogens. Among multi-bacterial determinants involving such ISR, the induction of ISR and promotion of growth by bacterial volatile compounds was previously reported. To exploit global de novo expression of plant proteins by bacterial volatiles, proteomic analysis was performed after exposure of Arabidopsis plants to the rhizobacterium Bacillus subtilis GB03. Ethylene biosynthesis enzymes were significantly up-regulated. Analysis by quantitative reverse transcriptase polymerase chain reaction confirmed that ethylene biosynthesis-related genes SAM-2, ACS4, ACS12, and ACO2 as well as ethylene response genes, ERF1, GST2, and CHIB were up-regulated by the exposure to bacterial volatiles. More interestingly, the emission of bacterial volatiles significantly up-regulated both key defense mechanisms mediated by jasmonic acid and salicylic acid signaling pathways. In addition, high accumulation of antioxidant proteins also provided evidence of decreased sensitivity to reactive oxygen species during the elicitation of ISR by bacterial volatiles. The present results suggest that the proteomic analysis of plant defense responses in bacterial volatile-mediated ISR can reveal the mechanisms of plant basal defenses orchestrated by endogenous ethylene production pathways and the generation of reactive oxygen species.

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Abbreviations

ET:

Ethylene

ISR:

Induced systemic resistance

JA:

Jasmonic acid

MS:

Murashige and Skoog

PGPR:

Plant growth-promoting rhizobacteria

qRT-PCR:

Quantitative RT-PCR

VOC:

Volatile organic compound

SA:

Salicylic acid

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Acknowledgments

This work was supported by an EB-NCRC grant (#R15-2003-012-02003-0) and the World Class University Program (#R32-10148) funded by MOEST, the 21C Frontier Microbial Genomics and Application Center Program, KRIBB initiative program, and partly by the Biogreen 21 program (20080401034023/20070401034005) funded by the Rural Development Administration, the Industrial Source Technology Development Program of the Ministry of Knowledge Economy (MKE) of Korea, and the National Academy of Agricultural Science, Rural Development Administration. Y.S.K. was supported by scholarships from the BK21 program funded by MOEST in Korea.

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Authors and Affiliations

  1. Department of Applied Biology and Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, South Korea

    Young Sang Kwon, Ki Soo Han, Jung Han Lee, Hee Kyu Kim & Dong-Won Bae

  2. Laboratory of Microbial Genomics, Industrial Biotechnology and Bioenergy Research Center, KRIBB, Daejeon, 305-806, South Korea

    Choong-Min Ryu, Soohyun Lee & Hyo Bee Park

  3. Department of Microbiology, College of Medicine, The Aging-associated Vascular Disease Research Center, Yeungnam University, Daegu, 707-717, South Korea

    Kyunghee Lee

  4. Division of Applied Life Science (BK21 program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Jinju, 660-701, South Korea

    Young Sang Kwon & Woo Sik Chung

  5. Bio-crop Development Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, South Korea

    Mi-Jeong Jeong

  6. Biosystems and Bioengineering Program, University of Science and Technology (UST), Yuseong-gu, Daejeon, 305-333, South Korea

    Choong-Min Ryu

  7. Central Instrument Facility, Gyeongsang National University, Jinju, 660-701, South Korea

    Dong-Won Bae

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  1. Young Sang Kwon
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Correspondence to Dong-Won Bae.

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Y. S. Kwon and C.-M. Ryu contributed equally to this work.

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Kwon, Y.S., Ryu, CM., Lee, S. et al. Proteome analysis of Arabidopsis seedlings exposed to bacterial volatiles. Planta 232, 1355–1370 (2010). https://doi.org/10.1007/s00425-010-1259-x

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