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Expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells, follicular fluid, and serum of women with polycystic ovary syndrome (PCOS)

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies that affects women in reproductive age. MicroRNAs (miRNAs) play crucial roles in normal function of female reproductive system and folliculogenesis. Deregulated expression of miRNAs in PCOS condition may be significantly implicated in the pathogenesis of PCOS. We determined relative expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells (GLCs), follicular fluid (FF), and serum of PCOS patients.

Methods

Human subjects were divided into PCOS (n = 20) and control (n = 21) groups. GLCs, FF, and serum were isolated and stored. RNA isolation was performed and cDNA was reversely transcribed using specific stem-loop RT primers. Relative expression of miRNAs was calculated after normalization against U6 expression. Correlation of miRNAs’ expression level with basic clinical features and predictive value of miRNAs in FF and serum were appraised.

Results

Relative expression of miR-145 and miR-182 in GLCs was significantly decreased in PCOS, but miR-182 in FF of PCOS patients revealed up-regulated levels. Significant correlations between level of miRNAs in FF and serum and hormonal profile of subjects were observed. MiR-182 in FF showed a significant predictive value with AUC of 0.73, 76.4% sensitivity, and 70.5% specificity which was improved after combination of miR-182 and miR-145.

Conclusions

A significant dysregulation of miR-145 and miR-182 in GLCs of PCOS may indicate their involvement in pathogenesis of PCOS. Differential up-regulation of miR-182 in FF of PCOS patients with its promising predictive values for discrimination of PCOS reinforced the importance of studying miRNAs’ profile in FF.

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Acknowledgements

This work was financially supported by the Research Deputy of Tehran University of Medical Sciences (Grant number: 92-02-30-22140).

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Naji, Saeid Nekoonam, Maryam Shabani Nashtaei & Fardin Amidi

  2. Department of Infertility, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Ashraf Aleyasin

  3. Molecular Virology Lab, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran

    Ehsan Arefian

  4. Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran

    Reza Mahdian

  5. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Elham Azizi

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Contributions

MN: protocol development, data collection, data analysis, and manuscript writing; SN: data collection and data analysis; AA: project development and data collection; EA: protocol development and data analysis; RM: protocol development and data analysis; EA: data collection and data analysis; MSN: manu writing; FA: project development, data analysis, and manuscript editing.

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Correspondence to Fardin Amidi.

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Conflict of interest

The authors of the current paper declare no conflict of interest regarding to all time periods of working—neither financial support nor personal relationship. Mohammad Naji declares that he has no conflict of interest. Saeid nekoonam declares that he has no conflict of interest. Ashraf Aleyasin declares that she has no conflict of interest. Ehsan Arefian declares that he has no conflict of interest. Reza Mahdian declares that he has no conflict of interest. Elham Azizi declares that she has no conflict of interest. Maryam Shabani Nashtaei declares that she has no conflict of interest. Fardin Amidi declares that he has no conflict of interest.

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Naji, M., Nekoonam, S., Aleyasin, A. et al. Expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells, follicular fluid, and serum of women with polycystic ovary syndrome (PCOS). Arch Gynecol Obstet 297, 221–231 (2018). https://doi.org/10.1007/s00404-017-4570-y

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