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Can adnexal torsion be predicted by measuring plasma heat shock protein 70 level? An experimental study

  • General Gynecology
  • Published:
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Abstract

Objective

To investigate the effect of adnexal torsion on the plasma heat shock protein 70 level and to determine whether plasma heat shock protein 70 can be used in the adnexal torsion diagnosis.

Materials and methods

Twenty-one nulligravid 3-month-old female Wistar albino rats were randomly and equally allocated into three groups: study group (ovarian torsion) (n = 7), laparotomy group (sham operation) (n = 7) and control group (received no special treatment) (n = 7). Ovarian torsion model was created by twisting the right adnexa two times around its pedicle and fixing over the lateral pelvis with 6.0 polyglactin absorbable surgical suture. Blood was sampled before and 12 h after operation to assess plasma heat shock protein 70 level.

Results

In the study group, the mean plasma heat shock protein 70 level was significantly higher than that in the laparotomy and control groups (1.75 ± 0.25), (1.16 ± 0.99), (1.19 ± 0.11) ng/ml, respectively, P = 0.001), following 12 h of ovarian torsion.

Conclusion

A significant increase in plasma heat shock protein 70 level in the study group indicates that plasma heat shock protein 70 level could be used as a serum marker in the early detection of adnexal torsion. However, further clinical and experimental studies of a larger size are required.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Kafkas University School of Medicine, Kars, Turkey

    Hasan Çılgın

  2. Department of Obstetrics and Gynecology, Firat University School of Medicine, Elazig, Turkey

    Mehmet Şimşek

  3. Department of Biophysics, Firat University School of Medicine, Elazig, Turkey

    Ramazan Bal

Authors
  1. Hasan Çılgın
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  2. Mehmet Şimşek
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  3. Ramazan Bal
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Contributions

HÇ: writing the paper, HÇ and RB: concept and design, MŞ: supervision.

Corresponding author

Correspondence to Hasan Çılgın.

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Ethical approval

The study was approved by the local Ethical Review Board of the Firat University Medical Faculty Elazıg, Turkey, for the use of laboratory animals, and was performed at the experimental surgery training and education center of the same hospital (On 13.08.2016, the ethics committee was approved with the number 58). All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Funding

This study was not funded by any organization.

Conflict of interest

All the authors of this manuscript declare that there is no conflict of interest.

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Çılgın, H., Şimşek, M. & Bal, R. Can adnexal torsion be predicted by measuring plasma heat shock protein 70 level? An experimental study. Arch Gynecol Obstet 296, 941–946 (2017). https://doi.org/10.1007/s00404-017-4502-x

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  • DOI: https://doi.org/10.1007/s00404-017-4502-x

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