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Down-regulation of soluble fms-like tyrosine kinase 1 expression in invasive placentation

  • Maternal-Fetal Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

To confirm reduced expression of soluble fms-like tyrosine kinase 1 (sFlt-1) in accreta/increta.

Methods

Formalin-fixed tissue sections from 11 peripartum hysterectomies with invasive placentation and 5 controls were stained for sFlt-1. Stain intensity was scored in selected 100× microscopic fields. We compared sFlt-1 expression in invasive areas among cases, non-invasive areas among cases and areas from control placentas.

Results

Chorionic villi displayed significantly decreased sFlt-1 expression in invasive areas of cases compared to control placentas (p = 0.003), as well as in non-invasive areas of cases compared to control placentas (p = 0.01). There was no difference in sFlt-1 expression between invasive and non-invasive areas among cases.

Conclusions

Expression of sFlt-1 is diminished in villous trophoblasts from patients with placenta increta or percreta. Local depth of invasion was not associated with sFlt-1 expression, suggesting a more global abnormality across the implantation site rather than localized to areas of histologic invasion.

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Author information

Authors and Affiliations

  1. Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Boston, USA

    Scott A. Shainker, S. Ananth Karumanchi, Michele R. Hacker & Jonathan L. Hecht

  2. Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA

    Scott A. Shainker & Kristin D. Gerson

  3. Clinical Fellow of Pathology, Harvard Medical School, Boston, USA

    Katelyn Dannheim

  4. Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA

    Katelyn Dannheim

  5. Clinical Fellow of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Boston, USA

    Kristin D. Gerson

  6. Program in Epidemiologic Research, Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA

    Dayna Neo & Michele R. Hacker

  7. Medicine, Harvard Medical School, Boston, USA

    Zsuzsanna K. Zsengeller

  8. Department of Molecular Medicine, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA

    Zsuzsanna K. Zsengeller

  9. Department of Molecular Medicine, Beth Israel Deaconess Medical Center, 99 Brookline Ave. Room # 359, Boston, MA, 02115, USA

    Elizabeth Pernicone

  10. Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA

    S. Ananth Karumanchi

  11. Gynecologic and Placental Pathology, Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA

    Jonathan L. Hecht

Authors
  1. Scott A. Shainker
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  2. Katelyn Dannheim
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  4. Dayna Neo
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  5. Zsuzsanna K. Zsengeller
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  7. S. Ananth Karumanchi
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Contributions

SAS, DO: Protocol/project development, Manuscript writing/editing. KD, MD: Data collection, Data management. KDG, MD, PhD: Data management. Ms. DN, MPH: Data management, Data analysis. ZKZ MD, PhD: Data collection. Ms. EP BS: Data collection. SAK, MBBS: Protocol/project development, Manuscript writing/editing. MRH, ScD, MSPH: Protocol/project development, Data analysis, Manuscript writing/editing. JLH, MD, PhD: Protocol/project development, Data collection, Manuscript writing/editing.

Corresponding author

Correspondence to Jonathan L. Hecht.

Ethics declarations

Funding

This study was funded by departmental discretionary funds.

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

Informed consent

Awaiver of informed consent was granted by the Institutional Review Board at Beth Israel Deaconess Medical Center, Boston, MA. The study is a retrospective analysis of preexisting clinical materials and deemed minimal risk.

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Shainker, S.A., Dannheim, K., Gerson, K.D. et al. Down-regulation of soluble fms-like tyrosine kinase 1 expression in invasive placentation. Arch Gynecol Obstet 296, 257–262 (2017). https://doi.org/10.1007/s00404-017-4432-7

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  • DOI: https://doi.org/10.1007/s00404-017-4432-7

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