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Vascular endothelial growth factor +405 C/G polymorphism is highly associated with an increased risk of endometriosis in Turkish women

  • General Gynecology
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Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Objective

Endometriosis is a chronic gynecological disease characterized by the growth of hormonally responsive, endometrial tissue outside the uterine cavity. The present study aims to analyze two vascular endothelial growth factor (VEGF) polymorphisms (−460 C/T and +405 C/G) in Turkish women with and without endometriosis.

Study design

A case–control study was undertaken at the Infertility Department of Zekai Tahir Burak Women’s Health Care Education and Research Hospital. The single nucleotide polymorphisms, −460 C/T and +405 C/G, in the 5′-untranslated region of the VEGF gene were tested in 98 affected women and 94 women with no laparoscopic evidence of disease. Endometriosis was also confirmed histologically. Following genomic extraction of genomic DNA, genotyping of the −460 C/T and +405 C/G polymorphisms of the VEGF gene were performed by polymerase chain reaction and restriction fragment length polymorphism assay. Nominal data were evaluated by Pearson Chi-square or Fisher’s Exact test, where applicable. Odds ratios and 95% confidence intervals were also calculated. A P value less than 0.05 was considered statistically significant.

Results

Demographic data were similar among groups. The genotype and allele frequencies of the −460 C/T polymorphism did not differ significantly between cases and controls. In contrast, the genotype (P < 0.001) and allele frequencies (P < 0.001) of +405 C/G polymorphism showed a significant difference between cases and controls. Regardless of the early or advanced stage, women with endometriosis showed a higher incidence of the +405 GC genotype and +405G allele when compared with the controls.

Conclusions

These data suggest that VEGF +405 GC genotype and +405G allele may be associated with the risk of developing early and advanced stage endometriosis in the Turkish population.

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Acknowledgments

These data were presented in part (poster presentation) at the 25th Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE 2009), which was held in Amsterdam, The Netherlands.

Conflict of interest statement

None.

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Authors and Affiliations

  1. Department of Infertility, Zekai Tahir Burak Women’s Health Care Education and Research Hospital, Ankara, Turkey

    S. Ozlem Altinkaya, Mustafa Ugur, Mustafa Ozat & Tayfun Gungor

  2. Intergen Genetics Center, Ankara, Turkey

    Gulay Ceylaner & Serdar Ceylaner

  3. İntergen Genetik Hastalıklar Tanı Merkezi, Konur İş Merkezi, İran Caddesi 13/25 Kavaklıdere, Çankaya, Ankara, Turkey

    S. Ozlem Altinkaya

Authors
  1. S. Ozlem Altinkaya
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  2. Mustafa Ugur
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  3. Gulay Ceylaner
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  4. Mustafa Ozat
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  5. Tayfun Gungor
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  6. Serdar Ceylaner
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Correspondence to S. Ozlem Altinkaya.

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Altinkaya, S.O., Ugur, M., Ceylaner, G. et al. Vascular endothelial growth factor +405 C/G polymorphism is highly associated with an increased risk of endometriosis in Turkish women. Arch Gynecol Obstet 283, 267–272 (2011). https://doi.org/10.1007/s00404-009-1344-1

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  • DOI: https://doi.org/10.1007/s00404-009-1344-1

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