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Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis

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Abstract

Glutathione (GSH) has been shown to negatively regulate methyl jasmonate (MeJA)-induced stomatal closure. We investigated the roles of GSH in MeJA signaling in guard cells using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase. MeJA-induced stomatal closure and decreased GSH contents in guard cells. Decreasing GSH by the cad2-1 mutation enhanced MeJA-induced stomatal closure. Depletion of GSH by the cad2-1 mutation or increment of GSH by GSH monoethyl ester did not affect either MeJA-induced production of reactive oxygen species (ROS) or MeJA-induced cytosolic alkalization in guard cells. MeJA and abscisic acid (ABA) induced stomatal closure and GSH depletion in atrbohD and atrbohF single mutants but not in the atrbohD atrbohF double mutant. Moreover, exogenous hydrogen peroxide induced stomatal closure but did not deplete GSH in guard cells. These results indicate that GSH affects MeJA signaling as well as ABA signaling and that GSH negatively regulates a signal component other than ROS production and cytosolic alkalization in MeJA signal pathway of Arabidopsis guard cells.

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Acknowledgments

This research was supported in part by a Grant-in-Aid for Young Scientists and Grants for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530, Japan

    Nasima Akter, Eiji Okuma, Muhammad Abdus Sobahan, Misugi Uraji, Shintaro Munemasa, Yoshimasa Nakamura & Yoshiyuki Murata

  2. Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki, Okayama, 710-0046, Japan

    Izumi C. Mori

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  1. Nasima Akter
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  2. Eiji Okuma
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  3. Muhammad Abdus Sobahan
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  5. Shintaro Munemasa
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  6. Yoshimasa Nakamura
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  7. Izumi C. Mori
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Correspondence to Yoshiyuki Murata.

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Akter, N., Okuma, E., Sobahan, M.A. et al. Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis . J Plant Growth Regul 32, 208–215 (2013). https://doi.org/10.1007/s00344-012-9291-7

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  • DOI: https://doi.org/10.1007/s00344-012-9291-7

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