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Dense populations of Archaea associated with the demosponge Tentorium semisuberites Schmidt, 1870 from Arctic deep-waters

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Abstract

The associated microbial community in the mesohyl of the Arctic deep-water sponge Tentorium semisuberites Schmidt, 1870 (Hadromerida, Demospongiae) is dominated by Archaea. This is the result of an integral approach applying analyses of microbial lipid biomarkers as well as microscopic investigations using differential fluorescence in situ hybridisation with universal probes and counterstaining with 4′,6′-diamidino-2-phenylindole (DAPI) on sponge sections based on samples collected in the Greenland Sea in 2001, 2002 and 2005. The distribution of isoprenoidal C40 hydrocarbons of the biphytane series suggests that affiliates of both major archaeal kingdoms, the Crenarchaeota and the Euryarchaeota, are present in the choanosome of T. semisuberites. Positive signals using the oligonucleotide probe ARCH915 indicate high numbers of Archaea in the mesohyl of this sponge. Based on optical estimations 70–90% of all microbial DAPI signals accounted for archaeal cells. Archaea in these high proportions have never been described in an Arctic deep-sea hadromerid sponge, nor in any other demosponge species. Similar observations in specimens collected over a time scale of 4 years suggest permanent sponge-Archaea associations.

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Acknowledgements

Sampling took place during annual revisits to the long term monitoring station “AWI-Hausgarten”. We thank the crews of the R/Vs “Polarstern” and “L′Atalante” for assistance with sampling, Sabine Beckmann for analytical work and Wolfgang Dröse as well as Angela Scharfbillig for preparation of sponge sections. Three anonymous reviewers have greatly improved the quality of the original manuscript. Andrew K. Sweetman is kindly acknowledged for proofreading of the manuscript. This paper represents publication no. 54 of the research program BOSMAN (03F0358 A and C). Financial support was provided by the Bundesministerium für Bildung und Forschung (BMBF), Germany. F.H. was funded by the Max Planck Society and by the Deutsche Forschungsgemeinschaft (DFG – Project HO 3293/1–1) during preparation of the manuscript.

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Author notes

  1. Friederike Hoffmann

    Present address: Max Planck Institute for Marine Microbiology, Celsiusstr. 1, 28359 , Bremen, Germany

  2. Karen von Juterzenka

    Present address: Institute for Polar Ecology, Christian-Albrechts-University of Kiel, Wischhofstr. 1-3 Geb. 12, 24148 , Kiel, Germany

  3. Thomas Pape

    Present address: Research Center Ocean Margins, University of Bremen, P.O. Box 330440, 28334, Bremen, Germany

Authors and Affiliations

  1. Institute of Biogeochemistry and Marine Chemistry, University of Hamburg, Bundesstr. 55, 20146, Hamburg, Germany

    Thomas Pape & Walter Michaelis

  2. Center of Geosciences, Geobiology, University of Göttingen, Goldschmidtstr. 3, 37077 , Göttingen, Germany

    Friederike Hoffmann & Joachim Reitner

  3. Alfred-Wegener-Institute for Polar and Marine Research, Deep Sea Research, Am Handelshafen 12, 27568 , Bremerhaven, Germany

    Nadia-Valérie Quéric & Karen von Juterzenka

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  1. Thomas Pape
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Correspondence to Thomas Pape.

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Pape, T., Hoffmann, F., Quéric, NV. et al. Dense populations of Archaea associated with the demosponge Tentorium semisuberites Schmidt, 1870 from Arctic deep-waters. Polar Biol 29, 662–667 (2006). https://doi.org/10.1007/s00300-005-0103-4

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  • DOI: https://doi.org/10.1007/s00300-005-0103-4

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