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Sequence diversity of mating-type genes in Phaeosphaeria avenaria

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Abstract.

Phaeosphaeria avenaria, one of the causal agents of stagonospora leaf blotch diseases in cereals, is composed of two subspecies, P. avenaria f. sp. triticea (Pat) and P. avenaria f. sp. avenaria (Paa). The Pat subspecies was grouped into Pat1–Pat3, based on restriction fragment length polymorphism (RFLP) and ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequences in previous studies. Mating-type genes and their potential use in phylogeny and molecular classification were studied by DNA hybridization and PCR amplification. The majority of Pat1 isolates reported to be homothallic and producing sexual reproduction structures on cultural media had only the MAT1-1 gene. Minor sequence variations were found in the conserved region of MAT1-1 gene in Pat1 isolates. However, both mating-type genes, MAT1-1 and MAT1-2, were identified in P. avenaria isolates represented by ATCC12277 from oats (Paa) and the Pat2 isolates from foxtail barley (Hordeum jubatum L.). Cluster analyses based on mating-type gene conserved regions revealed that cereal Phaeosphaeria is not phylogenetically closely related to other ascomycetes, including Mycosphaerella graminicola (anamorph Septoria tritici). The sequence diversity of mating-type genes in Pat and Paa supports our previous phylogenetic relationship and molecular classification based on RFLP fingerprinting and rDNA ITS sequences.

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Acknowledgements.

We thank Rosemarie Hammond of the USDA–ARS for reviewing the manuscript and Z. Ye of the University of Maryland for technical support. This research is partially supported by the USDA–ARS CRIS and the US–Poland Maria Sklodowska–Curie Joint Fund Projects.

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Authors and Affiliations

  1. Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, ARS, Beltsville, MD 20705, USA

    Peter P. Ueng

  2. Department of Biology, Shandong University, Jinan, Shandong, People's Republic of China

    Qun Dai

  3. State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou, Gansu, People's Republic of China

    Kai-rong Cui

  4. Department of Plant Pathology, Plant Breeding and Acclimatization Institute, Radzików, Poland

    Paweł C. Czembor & Edward Arseniuk

  5. Department of Plant Pathology, University of Georgia, Griffin, Ga., USA

    Barry M. Cunfer

  6. Department of Biochemistry, University of Maryland, College Park, Md., USA

    H. Tsang

  7. Department of Plant Pathology, Cornell University, Ithaca, N.Y., USA

    Gary C. Bergstrom

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  1. Peter P. Ueng
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  2. Qun Dai
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  3. Kai-rong Cui
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  4. Paweł C. Czembor
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  7. Edward Arseniuk
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Correspondence to Peter P. Ueng.

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Communicated by U. Kück

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Ueng, P.P., Dai, Q., Cui, Kr. et al. Sequence diversity of mating-type genes in Phaeosphaeria avenaria . Curr Genet 43, 121–130 (2003). https://doi.org/10.1007/s00294-003-0377-4

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  • DOI: https://doi.org/10.1007/s00294-003-0377-4

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