Skip to main content
SpringerLink
Log in

Description of Pseudomonas gregormendelii sp. nov., a Novel Psychrotrophic Bacterium from James Ross Island, Antarctica

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

During the microbiological research performed within the scope of activities of Czech expeditions based at the Johann Gregor Mendel Station at James Ross Island, Antarctica, two psychrotrophic gram-stain negative non-fluorescent strains CCM 8506T and CCM 8507 from soil were extensively characterized using genotypic and phenotypic methods. Initial characterization using ribotyping with HindIII restriction endonuclease and phenotyping implies that both isolates belong to a single Pseudomonas species. Sequencing of rrs, rpoB, rpoD and glnA genes of strain CCM 8506T confirmed affiliation of investigated strains within the genus Pseudomonas. Further investigation using automated ribotyping with EcoRI (RiboPrinter® Microbial Characterisation System), whole-cell protein profiling using the Agilent 2100 Bioanalyzer system, extensive biochemical testing and DNA–DNA hybridization experiments confirmed that both investigated strains are members of a single taxon which is clearly separated from all hitherto described Pseudomonas spp. Based on all findings, we describe a novel species Pseudomonas gregormendelii sp. nov. with the type strain CCM 8506T (=LMG 28632T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Achouak W, Laurent S, Thierry H, Meyer J-M, Fromin N, Degraeve S, Christen R, Gardan L (2000) Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana. Int J Syst Evol Microbiol 50:9–18

    Article  CAS  PubMed  Google Scholar 

  2. Bej AK, Aislabie J, Atlas RM (2010) Polar microbiology, the ecology, biodiversity and bioremediation potential of microorganisms in extremely cold environments. CRC Press, Boca Raton

    Google Scholar 

  3. Bozal N, Montes J, Mercadé E (2007) Pseudomonas guineae sp. nov., a novel psychrotolerant bacterium from an Antarctic environment. Int J Syst Evol Microbiol 57:2609–2612

    Article  CAS  PubMed  Google Scholar 

  4. Cleenwerck I, Vandemeulebroecke K, Janssens D, Swings J (2002) Reexamination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 52:1551–1558

    CAS  PubMed  Google Scholar 

  5. CLSI—Clinical and Laboratory Standards Institute (2011) Performance standards for antimicrobial susceptibility testing; 17th informational supplement. CLSI M100-S21 31. Clinical and Laboratory Standards Institute, Wayne

  6. Coenye T, Falsen E, Vancanneyt M, Hoste B, Govan JRW, Kersters K, Vandamme P (1999) Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Evol MicroBiol 49:405–413

    Google Scholar 

  7. Coroler L, Elomari M, Hoste B, Gillis M, Izard D, Leclerc H (1996) Pseudomonas rhodesiae sp. nov., a new species isolated from natural mineral waters. Syst Appl Microbiol 19:600–607

    Article  CAS  Google Scholar 

  8. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol MicroBiol 39:224–229

    Google Scholar 

  9. Goris J, Suzuki K, De Vos P, Nakase T, Kersters K (1998) Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153

    Article  CAS  Google Scholar 

  10. Hagstrom A, Pinhassi J, Zweifel UL (2000) Biogeographical diversity among marine bacterioplankton. Aquat Microb Ecol 21:231–244

    Article  Google Scholar 

  11. Huang X, Madan A (1999) CAP3: a DNA sequence assembly program. Genome Res 9:868–877

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Hughes MS, James G, Ball N, Scally M, Malik R, Wigney DI, Martin P, Chen S, Mitchell D, Love DN (2000) Identification by 16S rRNA gene analyses of a potential novel mycobacterial species as an etiological agent of canine leproid granuloma syndrome. J Clin Microbiol 38:953–959

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Jones D, Pell PA, Sneath PHA (1991) Maintenance of bacteria on glass beads at −60 to −76 °C. In: Kirsop BE, Doyle A (eds) Maintenance of microorganism and cultured cells. A manual of laboratory methods, 2nd edn. Academic Press, London, pp 45–50

    Google Scholar 

  14. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  15. Kosina M, Barták M, Mašlaňová I, Vávrová-Pascutti A, Šedo O, Lexa M, Sedláček I (2013) Pseudomonas prosekii sp. nov., a novel psychrotrophic bacterium from Antarctica. Curr Microbiol 67(6):637–664. doi:10.1007/s00284-013-0406-6

    Article  CAS  PubMed  Google Scholar 

  16. López NI, Pettinari MJ, Stackebrandt E, Tribelli PM, Pötter M, Steinbüchel A, Méndez BS (2009) Pseudomonas extremaustralis sp.nov., a Poly(3-hydroxybutyrate) producer isolated from an Antarctic environment. Curr Microbiol 59:514–519

    Article  PubMed  Google Scholar 

  17. Mesbah M, Whitman WB (1989) Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr A 479:297–306

    Article  CAS  Google Scholar 

  18. Mulet M, Lalucat J, García-Valdés E (2010) DNA sequence-based analysis of the Pseudomonas species. Environ Microbiol 12:1513–1530

    CAS  PubMed  Google Scholar 

  19. Mulet M, Bennasar A, Lalucat J, García-Valdés E (2009) An rpoD-based PCR procedure for the identification of Pseudomonas species and for their detection in environmental samples. Mol Cell Probes 23:140–147

    Article  CAS  PubMed  Google Scholar 

  20. Onofri S, Selbmann L, Zucconi L, Pagano S (2004) Antarctic microfungi as models for exobiology. Planet Space Sci. doi:10.1016/j.pss.2003.08.019

    Google Scholar 

  21. Reddy GSN, Matsumoto GI, Shuman P, Stackebrandt E, Shivaji S (2004) Psychrophilic Pseudomonas from Antarctica: Pseudomonas antarctica sp. nov., Pseudomonas meridianae sp. nov. and Pseudomonas proteolytica sp. nov. Int J Syst Evol Microbiol 54:713–719

    Article  CAS  PubMed  Google Scholar 

  22. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  24. Shivaji S, Rao NS, Saisree L, Sheth V, Reddy GSN, Bhargava PM (1989) Isolation and identification of Pseudomonas spp. from schirmacher oasis, Antarctica. Appl Environ Microbiol 55:767–770

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Švec P, Sedláček I, Pantůček R, Devriese LA, Doškař J (2001) Evaluation of ribotyping for characterization and identification of Enterococcus haemoperoxidus and Enterococcus moraviensis strains. FEMS Microbiol Lett 203:23–27

    Article  PubMed  Google Scholar 

  26. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  27. Tayeb AL, Ageron E, Grimont F, Grimont PA (2005) Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates. Res Microbiol 156:763–773

    Article  Google Scholar 

  28. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Verhille S, Baida N, Dabboussi F, Hamze M, Izard D, Leclerc H (1999) Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov., two new species isolated from natural mineral waters. Int J Syst Evol Microbiol 49:1559–1572

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic, Project CZ.1.07/2.3.00/20.0183 and CzechPolar project (LM2010009). Special acknowledgement belongs to Dr. Jean Paul Euzéby for taxonomic name revision.

Author information

Authors and Affiliations

  1. Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, Brno, Czech Republic

    Marcel Kosina, Pavel Švec, Jitka Černohlávková, Miloš Barták & Ivo Sedláček

  2. Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, Czech Republic

    Kateřina Snopková

  3. Lab of Microbiology, UGent, Ghent, Belgium

    Paul De Vos

Authors
  1. Marcel Kosina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pavel Švec
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jitka Černohlávková
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Miloš Barták
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kateřina Snopková
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Paul De Vos
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ivo Sedláček
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marcel Kosina.

Electronic Supplementary Material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kosina, M., Švec, P., Černohlávková, J. et al. Description of Pseudomonas gregormendelii sp. nov., a Novel Psychrotrophic Bacterium from James Ross Island, Antarctica. Curr Microbiol 73, 84–90 (2016). https://doi.org/10.1007/s00284-016-1029-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-016-1029-5

Keywords

Search

Navigation