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Characterization of Comamonas thiooxidans sp. nov., and Comparison of Thiosulfate Oxidation with Comamonas testosteroni and Comamonas composti

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Abstract

Comamonas thiooxidans (strain S23T) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA–DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990T and 52% with Comamonas composti LMG24008T, the nearest phylogenetic relative (16S rRNA sequence similarity <97%). Comparative genomic fingerprinting by using ERIC and Rep-PCR further delineated species identity of the strain S23T for which Comamonas thiooxidans sp. nov. is proposed. In addition, thiosulfate oxidation potential of the strain S23T was compared with Comamonas testosteroni and Comamonas composti.

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Acknowledgments

We are grateful to Dr. P. Schumann, DSMZ of Germany, for his help in determining the G + C content composition of the organism. We are also thankful to Korean Collection for Type Cultures (KCTC), Daejeon, Republic of Korea, and BCCMTM/LMG Bacteria collection, Universiteit Gent, Belgium for providing us the type strains C. testosteroni KCTC2990T and Comamonas composti LMG24008T used in this study. This study was supported by the Department of Biotechnology, Ministry of Science and Technology, Government of India.

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  1. Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751 023, India

    Kunwar Digvijay Narayan, Shachindra K. Pandey & Subrata K. Das

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  1. Kunwar Digvijay Narayan
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  2. Shachindra K. Pandey
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  3. Subrata K. Das
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Correspondence to Subrata K. Das.

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Narayan, K.D., Pandey, S.K. & Das, S.K. Characterization of Comamonas thiooxidans sp. nov., and Comparison of Thiosulfate Oxidation with Comamonas testosteroni and Comamonas composti . Curr Microbiol 61, 248–253 (2010). https://doi.org/10.1007/s00284-010-9602-9

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