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Enhanced thermal stability and specific activity of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling amphipathic peptides

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Abstract

Self-assembling amphipathic peptides (SAPs) are a category of peptides that have unique sequences with alternating hydrophobic and hydrophilic residues that can spontaneously assemble into ordered nanostructures. In this study, we investigated the potential of fusion technique with SAPs to improve the thermal stability of lipoxygenase (LOX) from Pseudomonas aeruginosa. Six SAPs were individually fused to the N terminus of the LOX that resulted to the SAP–LOX fusions with approximately 2.3- to 4.5-fold enhanced thermal stability at 50 °C. The specific activities of the SAP–LOX fusions were also increased by 1.0- to 2.8-fold as compared with the wild-type LOX. This is the first report on the improvement of the thermal stability and specific activity of an enzyme by the fused SAPs, suggesting a simple technique to improve the catalytic properties of the recombinant enzymes by fusion expression.

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Acknowledgments

We would like to thank Professor Byong H. Lee for critical reading and suggestions on the manuscript. This work was supported by the National Natural Science Foundation of China (no. 31171639, no. 31000031, and no. 31070711), the National High Technology Research and Development Program of China (No. 2011AA100905), the NCET-10-0461, the Natural Science Foundation of Jiangsu Province (No. BK2010147), and the Independent Innovation Program of Jiangnan University (JUSRP11215).

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Xinyao Lu, Song Liu, Dongxu Zhang & Yi Liu

  2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China

    Jian Chen

  3. School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Xinyao Lu, Song Liu, Dongxu Zhang, Xiaoman Zhou, Yi Liu, Jing Wu, Guocheng Du & Jian Chen

  4. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Miao Wang & Jing Wu

  5. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Guocheng Du

  6. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Miao Wang, Guocheng Du & Jian Chen

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  1. Xinyao Lu
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Correspondence to Jing Wu or Jian Chen.

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Lu, X., Liu, S., Zhang, D. et al. Enhanced thermal stability and specific activity of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling amphipathic peptides. Appl Microbiol Biotechnol 97, 9419–9427 (2013). https://doi.org/10.1007/s00253-013-4751-y

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  • DOI: https://doi.org/10.1007/s00253-013-4751-y

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