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Involvement of a putative response regulator Brrg-1 in the regulation of sporulation, sensitivity to fungicides, and osmotic stress in Botrytis cinerea

  • Applied Genetics and Molecular Biotechnology
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Abstract

The response regulator protein is a core element of two-component signaling pathway. In this study, we investigated functions of BRRG-1 of Botrytis cinerea, a gene that encodes a putative response regulator protein, which is homologous to Rrg-1 in Neurospora crassa. The BRRG-1 gene deletion mutant ΔBrrg1-62 was unable to produce conidia. The mutant showed increased sensitivity to osmotic stress mediated by NaCl and KCl, and to oxidative stress generated by H2O2. Additionally, the mutant was more sensitive to the fungicides iprodione, fludioxonil, and triadimefon than the parental strain. Western-blot analysis showed that the Bos-2 protein, the putative downstream component of Brrg-1, was not phosphorylated in the ΔBrrg1-62. Real-time polymerase chain reaction assays showed that expression of BOS-2 also decreased significantly in the mutant. All of the defects were restored by genetic complementation of the ΔBrrg1-62 with the wild-type BRRG-1 gene. Plant inoculation tests showed that the mutant did not show changes in pathogenicity on rapeseed leaves. These results indicated that Brrg-1 is involved in the regulation of asexual development, sensitivity to iprodione, fludioxonil, and triadimefon fungicides, and adaptation to osmotic and oxidative stresses in B. cinerea.

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Acknowledgements

This research was supported by the fund from Modern Agro-industry Technology Research System and National Science Foundation (30771430) to Z. Ma.

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Authors and Affiliations

  1. Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 268 Kaixuan Road, Hangzhou, 310029, China

    Leiyan Yan, Qianqian Yang, Jinhua Jiang & Zhonghua Ma

  2. Department of Plant Pathology, Kearney Agricultural Center, University of California Davis, Parlier, CA, 93648, USA

    Themis J. Michailides

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  1. Leiyan Yan
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  2. Qianqian Yang
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  3. Jinhua Jiang
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  4. Themis J. Michailides
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  5. Zhonghua Ma
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Correspondence to Zhonghua Ma.

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Yan, L., Yang, Q., Jiang, J. et al. Involvement of a putative response regulator Brrg-1 in the regulation of sporulation, sensitivity to fungicides, and osmotic stress in Botrytis cinerea . Appl Microbiol Biotechnol 90, 215–226 (2011). https://doi.org/10.1007/s00253-010-3027-z

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