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Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation

  • Applied Microbial and Cell Physiology
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Abstract

Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of different doses of N+ implantation. The optimum dose of ion implantation for the Bs-916 was from 15 × 2.6 × 1014 N+/cm2 to 25 × 2.6 × 1014 N+/cm2. The mutant strain designated as Bs-H74 was obtained, which showed higher inhibition activity in the screening plate. Its inhibition zone against the indicator organism increased by 30.7% compared to the parental strain. The control effect of rice sheath blight was improved by 14.6% over that of Bs-916. Thin-layer chromatography and high-performance liquid chromatography analysis indicated that lipopeptides produced by Bs-916 and the mutant strains belonged to the surfactin family. Bs-H74 produced approximately 3.0-fold surfactin compared to that of Bs-916. To determine the role of surfactin in biocontrol by Bs-916, we tested another mutant strain, Bs-M49, which produced lower levels of surfactin significantly, and found that Bs-M49 had no obvious effects against R. solani. These results suggested that the surfactin produced by Bs-916 plays an important role in the suppression of sheath blight. These observations also showed that the Bs-H74 mutant strain is a better biocontrol agent than the parental strain.

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Acknowledgment

This research was support by funding from the Span Plan of National agricultural Science and Technology of china grant no. 2003-18 and by grant no.2002ZB0114 from the Key Science and Technology Program of Jiangsu.

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Authors and Affiliations

  1. Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China

    Dequan Li, Fengya Nie, Lihui Wei & Benqiang Wei

  2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Zhiyi Chen

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  1. Dequan Li
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  2. Fengya Nie
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  3. Lihui Wei
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  4. Benqiang Wei
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Correspondence to Zhiyi Chen.

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Li, D., Nie, F., Wei, L. et al. Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation. Appl Microbiol Biotechnol 75, 1401–1408 (2007). https://doi.org/10.1007/s00253-007-0951-7

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  • DOI: https://doi.org/10.1007/s00253-007-0951-7

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