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Biotechnological aspects of chitinolytic enzymes: a review

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Abstract

Chitin and chitinases (EC 3.2.1.14) have an immense potential. Chitinolytic enzymes have wide-ranging applications such as preparation of pharmaceutically important chitooligosaccharides and N-acetyl d-glucosamine, preparation of single-cell protein, isolation of protoplasts from fungi and yeast, control of pathogenic fungi, treatment of chitinous waste, and control of malaria transmission. In this review, we discuss the occurrence and structure of chitin, the types and sources of chitinases, their mode of action, chitinase production, as well as molecular cloning and protein engineering of chitinases and their biotechnological applications.

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Acknowledgement

The authors thank the Council of Scientific and Industrial Research (CSIR), Government of India for financial support in carrying out these investigations.

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Authors and Affiliations

  1. Genes and Proteins Laboratory, National Institute of Immunology, Aruna Ashaf Ali Marg, J.N.U. Campus, New Delhi, 110067, India

    Neetu Dahiya

  2. Department of Biotechnology, Panjab University, Chandigarh, 160014, India

    Rupinder Tewari

  3. Department of Microbiology, Panjab University, Chandigarh, 160014, India

    Gurinder Singh Hoondal

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  1. Neetu Dahiya
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Correspondence to Neetu Dahiya.

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Dahiya, N., Tewari, R. & Hoondal, G.S. Biotechnological aspects of chitinolytic enzymes: a review. Appl Microbiol Biotechnol 71, 773–782 (2006). https://doi.org/10.1007/s00253-005-0183-7

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