Abstract
Biofilms, and other bacterial aggregations, are of significance in both environmental microbiology and in plant and human pathogenesis. Comparative single-cell Raman spectral analysis can differentiate between planktonic bacteria and those recovered from biofilms and appears to offer a new means by which to investigate bacterial cell physiology, metabolic status, and stress under different environmental conditions.
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Acknowledgements
We are grateful to Andrew Knowles and Simon FitzGerald from HORIBA Jobin-Yvon Ltd. UK for supplying the Raman Confocal Microscope.
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Huang, W.E., Ude, S. & Spiers, A.J. Pseudomonas fluorescens SBW25 Biofilm and Planktonic Cells Have Differentiable Raman Spectral Profiles. Microb Ecol 53, 471–474 (2007). https://doi.org/10.1007/s00248-006-9190-1
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DOI: https://doi.org/10.1007/s00248-006-9190-1