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Enrichment of serum biomarkers by magnetic metal-organic framework composites

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Abstract

Highly efficient extraction of peptides from serum is critical for finding serum biomarkers using mass spectrometry, which still remains a great challenge. Currently, a bottom-up proteomics approach has been applied to discover serum biomarkers. However, the approach was labor intensive, time and cost consuming, and cannot meet the requirements for clinical application. In this work, Fe3O4/C@MIL-100 composites were synthesized to efficiently capture peptides from microwave-assisted formic acid digests of BSA and human serum prior to MALDI-TOF MS analysis. Fe3O4/C@MIL-100 composites exhibited size-selective adsorption performance, thus providing a rapid and convenient approach to enrich low-abundance peptides. Notably, the peptides’ mass fingerprinting of serum digestions between type 2 diabetes mellitus (T2DM) and healthy persons were distinguishable, which indicated the potential ability of this technique for T2DM diagnosis and rapid biomarker discovery.

Efficient extraction and identification of serum biomarkers using Fe3O4/C@MIL-100 composites from acid hydrolysate

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (grants 21475095, 21106101, 81473472) and the Tianjin Natural Science Foundation (12JCZDJC29500, 13JCQNJC06300). Also, we are thankful to Technology Support Center of Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences for providing the use of experimental instruments and materials.

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Authors and Affiliations

  1. Tianjin Modern Vocational Technology College, Tianjin, 300350, China

    Ji-Ping Wei

  2. Nankai Hospital of Tianjin City, Tianjin, 300100, China

    Heng Wang

  3. School of Computer Science and Technology, Tianjin Key Laboratory of Cognitive Computing and Application, Tianjin University, Tianjin, 300072, China

    Tao Luo

  4. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Zhi-Jiang Zhou & Bin Qiao

  5. College of Environmental and Chemical Engineering Sciences, Tianjin Polytechnic University, Tianjin, 300387, China

    Yan-Feng Huang

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  1. Ji-Ping Wei
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  2. Heng Wang
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  3. Tao Luo
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  4. Zhi-Jiang Zhou
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  5. Yan-Feng Huang
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  6. Bin Qiao
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Correspondence to Yan-Feng Huang or Bin Qiao.

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All procedures performed in studies involving human participants (healthy volunteers and patients) have been approved by the ethics committee and have been performed in accordance with ethical standards. Informed consent was obtained from all individual participants included in the study. This article does not contain any studies with animals performed by any of the authors.

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Wei, JP., Wang, H., Luo, T. et al. Enrichment of serum biomarkers by magnetic metal-organic framework composites. Anal Bioanal Chem 409, 1895–1904 (2017). https://doi.org/10.1007/s00216-016-0136-2

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  • DOI: https://doi.org/10.1007/s00216-016-0136-2

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