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A new ELISA for the quantification of equine procalcitonin in plasma as potential inflammation biomarker in horses

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Abstract

In human medicine, procalcitonin (PCT) is a very common and well-established biomarker for sepsis. Even though sepsis is also a leading cause of death in foals and adult horses, up to now, no data about the role of equine PCT in septic horses has been available. Based on monoclonal antibodies targeted against human PCT, we report here the development of a sandwich ELISA for the quantification of equine PCT in equine plasma samples. The ELISA was characterized for intra- and interassay variance and a working range from 25 to 1,000 ng mL−1 was defined as within this range; both intra- and interassay variances were below 15 %. The target recovery ranged between 73 and 106 %. The ELISA was used to determine the equine PCT concentration in 24 healthy and 5 septic horses to show the potential for clinical evaluation of equine PCT. Significantly different (P = 0.0006) mean equine PCT concentrations were found for the healthy control group and the sepsis group (47 and 8,450 ng mL−1).

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Authors and Affiliations

  1. Research Unit Microbe-Plant Interactions, Department of Environmental Sciences, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstraße 1, 85764, Neuherberg, Germany

    Martin Rieger, Christian Kochleus, Daniela Rascher, Michael Schmid & Anton Hartmann

  2. Equine Clinic for Surgery and Radiology, Free University of Berlin, Oertzenweg 19b, 14163, Berlin, Germany

    Dana Teschner, Ann Kristin Barton & Heidrun Gehlen

  3. Institute of Structural Biology, Protein Expression & Purification Facility, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstraße 1, 85764, Neuherberg, Germany

    Arie Geerlof

  4. Institute of Molecular Immunology, Service Unit Monoclonal Antibodies, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstraße 1, 85764, Neuherberg, Germany

    Elisabeth Kremmer

Authors
  1. Martin Rieger
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  2. Christian Kochleus
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  3. Dana Teschner
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  4. Daniela Rascher
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  5. Ann Kristin Barton
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  6. Arie Geerlof
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  7. Elisabeth Kremmer
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  8. Michael Schmid
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  9. Anton Hartmann
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  10. Heidrun Gehlen
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Corresponding author

Correspondence to Martin Rieger.

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Rieger, M., Kochleus, C., Teschner, D. et al. A new ELISA for the quantification of equine procalcitonin in plasma as potential inflammation biomarker in horses. Anal Bioanal Chem 406, 5507–5512 (2014). https://doi.org/10.1007/s00216-014-7944-z

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  • DOI: https://doi.org/10.1007/s00216-014-7944-z

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